Engineering of the terpenoid pathway in Saccharomyces cerevisiae co-overproduces squalene and the non-terpenoid compound oleic acid

2016 ◽  
Vol 152 ◽  
pp. 457-467 ◽  
Author(s):  
Aamir Rasool ◽  
Genlin Zhang ◽  
Zhe Li ◽  
Chun Li
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Terpenoid Pathway

Oleic acid and ergosterol supplementation mitigates oxidative stress in wine strains of Saccharomyces cerevisiae

2010 ◽  
Vol 141 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Sara Landolfo ◽  
Giacomo Zara ◽  
Severino Zara ◽  
Marilena Budroni ◽  
Maurizio Ciani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Oleic Acid

scholarly journals Synthesis of Polyhydroxyalkanoate in the Peroxisome of Saccharomyces cerevisiae by Using Intermediates of Fatty Acid β-Oxidation

2001 ◽  
Vol 67 (11) ◽  
pp. 5254-5260 ◽  
Author(s):  
Yves Poirier ◽  
Nadine Erard ◽  
Jean MacDonald-Comber Petétot
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Isocitrate Lyase ◽  
Dry Weight ◽  
Pha Synthase ◽  
Recombinant Yeast ◽  
Medium Chain Length ◽  
The Media

ABSTRACT Medium-chain-length polyhydroxyalkanoates (PHAs) are polyesters having properties of biodegradable thermoplastics and elastomers that are naturally produced by a variety of pseudomonads.Saccharomyces cerevisiae was transformed with thePseudomonas aeruginosa PHAC1 synthase modified for peroxisome targeting by the addition of the carboxyl 34 amino acids from the Brassica napus isocitrate lyase. The PHAC1 gene was put under the control of the promoter of the catalase A gene. PHA synthase expression and PHA accumulation were found in recombinantS. cerevisiae growing in media containing fatty acids. PHA containing even-chain monomers from 6 to 14 carbons was found in recombinant yeast grown on oleic acid, while odd-chain monomers from 5 to 15 carbons were found in PHA from yeast grown on heptadecenoic acid. The maximum amount of PHA accumulated was 0.45% of the dry weight. Transmission electron microscopy of recombinant yeast grown on oleic acid revealed the presence of numerous PHA inclusions found within membrane-bound organelles. Together, these data show that S. cerevisiae expressing a peroxisomal PHA synthase produces PHA in the peroxisome using the 3-hydroxyacyl coenzyme A intermediates of the β-oxidation of fatty acids present in the media. S. cerevisiaecan thus be used as a powerful model system to learn how fatty acid metabolism can be modified in order to synthesize high amounts of PHA in eukaryotes, including plants.

scholarly journals Transcriptomic Response of Saccharomyces cerevisiae during Fermentation under Oleic Acid and Ergosterol Depletion

Fermentation ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 57 ◽  
Author(s):  
Giacomo Zara ◽  
Hennie J. J. van Vuuren ◽  
Ilaria Mannazzu ◽  
Severino Zara ◽  
Marilena Budroni
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Lipid Fraction ◽  
Membrane Lipid ◽  
Ergosterol Biosynthesis ◽  
Methionine Biosynthesis ◽  
Transcriptomic Response ◽  
Hypoxic Conditions ◽  
Lipid Supplementation ◽  
Time Point

Under anaerobic/hypoxic conditions, Saccharomyces cerevisiae relies on external lipid supplements to modulate membrane lipid fraction in response to different stresses. Here, transcriptomic responses of two S. cerevisiae wine strains were evaluated during hypoxic fermentation of a synthetic must with/without ergosterol and oleic acid supplementation. In the absence of lipids, the two strains, namely EC1118 and M25, showed different behaviour, with M25 significantly decreasing its fermentation rate from the 72 h after inoculum. At this time point, the whole genome transcriptomic analysis revealed common and strain-specific responses to the lack of lipid supplementation. Common responses included the upregulation of the genes involved in ergosterol biosynthesis, as well as the seripauperin and the heat shock protein multigene families. In addition, the upregulation of the aerobic isoforms of genes involved in mitochondrial electron transport is compatible with the previously observed accumulation of reactive oxygen species in the two strains during growth in absence of lipids. Considering the strain-specific responses, M25 downregulated the transcription of genes involved in glucose transport, methionine biosynthesis and of those encoding mannoproteins required for adaptation to low temperatures and hypoxia. The identification of these pathways, which are presumably involved in yeast resistance to stresses, will assist industrial strain selection.

scholarly journals Giant peroxisomes in oleic acid-induced Saccharomyces cerevisiae lacking the peroxisomal membrane protein Pmp27p.

1995 ◽  
Vol 128 (4) ◽  
pp. 509-523 ◽  
Author(s):  
R Erdmann ◽  
G Blobel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Growth Defect ◽  
Wild Type ◽  
Peroxisomal Membrane ◽  
Daughter Cells ◽  
Sds Page ◽  
Multiple Buds ◽  
Media Form ◽  
Tubular Membranes

We have purified peroxisomal membranes from Saccharomyces cerevisiae after induction of peroxisomes in oleic acid-containing media. About 30 distinct proteins could be discerned among the HPLC- and SDS-PAGE-separated proteins of the high salt-extracted peroxisomal membranes. The most abundant of these, Pmp27p, was purified and the corresponding gene PMP27 was cloned and sequenced. Its primary structure is 32% identical to PMP31 and PMP32 of the yeast Candida biodinii (Moreno, M., R. Lark, K. L. Campbell, and M. J. Goodman. 1994. Yeast. 10:1447-1457). Immunoelectron microscopic localization of Pmp27p showed labeling of the peroxisomal membrane, but also of matrix-less and matrix containing tubular membranes nearby. Electronmicroscopical data suggest that some of these tubular extensions might interconnect peroxisomes to form a peroxisomal reticulum. Cells with a disrupted PMP27 gene (delta pmp27) still grew well on glucose or ethanol, but they failed to grow on oleate although peroxisomes were still induced by transfer to oleate-containing media. The induced peroxisomes of delta pmp27 cells were fewer but considerably larger than those of wild-type cells, suggesting that Pmp27p may be involved in parceling of peroxisomes into regular quanta. delta pmp27 cells cultured in oleate-containing media form multiple buds, of which virtually all are peroxisome deficient. The growth defect of delta pmp27 cells on oleic acid appears to result from the inability to segregate the giant peroxisomes to daughter cells.

scholarly journals Saccharomyces cerevisiae PIP2Mediating Oleic Acid Induction and Peroxisome Proliferation Is Regulated by Adr1p and Pip2p-Oaf1p

2003 ◽  
Vol 278 (30) ◽  
pp. 27605-27611 ◽  
Author(s):  
Hanspeter Rottensteiner ◽  
Leila Wabnegger ◽  
Ralf Erdmann ◽  
Barbara Hamilton ◽  
Helmut Ruis ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Peroxisome Proliferation

scholarly journals An efficient positive selection procedure for the isolation of peroxisomal import and peroxisome assembly mutants of Saccharomyces cerevisiae.

Genetics ◽  
1993 ◽  
Vol 135 (3) ◽  
pp. 731-740 ◽  
Author(s):  
Y Elgersma ◽  
M van den Berg ◽  
H F Tabak ◽  
B Distel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Protein Import ◽  
Chimeric Protein ◽  
Selection Procedure ◽  
Peroxisome Biogenesis ◽  
Wild Type ◽  
Peroxisomal Protein ◽  
Complementation Groups ◽  
Resistance Protein

Abstract To study peroxisome biogenesis, we developed a procedure to select for Saccharomyces cerevisiae mutants defective in peroxisomal protein import or peroxisome assembly. For this purpose, a chimeric gene was constructed encoding the bleomycin resistance protein linked to the peroxisomal protein luciferase. In wild-type cells this chimeric protein is imported into the peroxisome, which prevents the neutralizing interaction of the chimeric protein with its toxic phleomycin ligand. Peroxisomal import and peroxisome assembly mutants are unable to import this chimeric protein into their peroxisomes. This enables the bleomycin moiety of the chimeric protein to bind phleomycin, thereby preventing its toxicity. The selection is very efficient: upon mutagenesis, 84 (10%) of 800 phleomycin resistant colonies tested were unable to grow on oleic acid. This rate could be increased to 25% using more stringent selection conditions. The selection procedure is very specific; all oleic acid non utilizing (onu) mutants tested were disturbed in peroxisomal import and/or peroxisome assembly. The pas (peroxisome assembly) mutants that have been used for complementation analysis represent 12 complementation groups including three novel ones, designated pas20, pas21 and pas22.

scholarly journals Fluidity and Lipid Composition of Membranes of Peroxisomes, Mitochondria and the ER From Oleic Acid-Induced Saccharomyces cerevisiae

2020 ◽  
Vol 8 ◽  
Author(s):  
Katharina Reglinski ◽  
Laura Steinfort-Effelsberg ◽  
Erdinc Sezgin ◽  
Christian Klose ◽  
Harald W. Platta ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Lipid Composition

scholarly journals Studies on the effect of an heterologous fatty acid-binding protein on acyl-CoA oxidase induction in Saccharomyces cerevisiae

1994 ◽  
Vol 301 (2) ◽  
pp. 615-620 ◽  
Author(s):  
I Smaczyńska ◽  
M Skoneczny ◽  
A Kurlandzka
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Yeast Cells ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Acyl Coa Oxidase

The participation of fatty acid-binding protein (FABP) in the induction of peroxisomal beta-oxidation of fatty acids was investigated in vivo in an heterologous system. Bovine heart FABP was expressed in Saccharomyces cerevisiae under the control of two different promoters: a constitutive one and an oleic acid-inducible one. Constructs were introduced into yeast cells on multicopy and integrating plasmids. The heterologous FABP was present in yeast cells in two isoforms having pI values of about 5 and was able to bind oleic acid. The heterologous FABP had no significant effect on acyl-CoA oxidase activity at various concentrations of the inducing agent.

scholarly journals Growth of catalase A and catalase T deficient mutant strains of Saccharomyces cerevisiae on ethanol and oleic acid

1990 ◽  
Vol 153 (5) ◽  
pp. 513-517 ◽  
Author(s):  
Ida J. van der Klei ◽  
Joanna Rytka ◽  
Wolf H. Kunau ◽  
Marten Veenhuis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Deficient Mutant ◽  
Mutant Strains
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