scholarly journals Growth of catalase A and catalase T deficient mutant strains of Saccharomyces cerevisiae on ethanol and oleic acid

1990 ◽  
Vol 153 (5) ◽  
pp. 513-517 ◽  
Author(s):  
Ida J. van der Klei ◽  
Joanna Rytka ◽  
Wolf H. Kunau ◽  
Marten Veenhuis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Deficient Mutant ◽  
Mutant Strains

Multiple-yapsin-deficient mutant strains for high-level production of intact recombinant proteins in Saccharomyces cerevisiae

2010 ◽  
Vol 149 (1-2) ◽  
pp. 1-7 ◽  
Author(s):  
Eun Young Cho ◽  
Seon Ah Cheon ◽  
Hyunah Kim ◽  
Jinho Choo ◽  
Dong-Jik Lee ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Recombinant Proteins ◽  
Deficient Mutant ◽  
Mutant Strains ◽  
High Level ◽  
Level Production

Oleic acid and ergosterol supplementation mitigates oxidative stress in wine strains of Saccharomyces cerevisiae

2010 ◽  
Vol 141 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Sara Landolfo ◽  
Giacomo Zara ◽  
Severino Zara ◽  
Marilena Budroni ◽  
Maurizio Ciani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Oleic Acid

scholarly journals Adenosine kinase-deficient mutant of Neurospora crassa

1982 ◽  
Vol 152 (3) ◽  
pp. 1292-1294
Author(s):  
J M Magill ◽  
P Dalke ◽  
T S Lyda ◽  
C W Magill
Keyword(s):  
Neurospora Crassa ◽  
Resistant Mutant ◽  
Adenosine Kinase ◽  
Deficient Mutant ◽  
Wild Type ◽  
Purine Nucleotides ◽  
Mutant Strains

Tubercidin-resistant mutant strains of Neurospora crassa were isolated, and at least one appeared to be deficient in adenosine kinase. No significant differences in [8-14C]adenosine labeling of purine nucleotides or nucleosides were found between the wild type and the adenosine kinase-deficient strains.

scholarly journals The essential DNA polymerases delta and epsilon are involved in repair of UV-damaged DNA in the yeast Saccharomyces cerevisiae.

1999 ◽  
Vol 46 (2) ◽  
pp. 289-298 ◽  
Author(s):  
A Hałas ◽  
Z Policińska ◽  
H Baranowska ◽  
W J Jachymczyk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Uv Irradiation ◽  
Dna Polymerases ◽  
Relative Molecular Mass ◽  
Yeast Saccharomyces Cerevisiae ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Mutant Strains ◽  
Cellular Dna

We have studied the ability of yeast DNA polymerases to carry out repair of lesions caused by UV irradiation in Saccharomyces cerevisiae. By the analysis of postirradiation relative molecular mass changes in cellular DNA of different DNA polymerases mutant strains, it was established that mutations in DNA polymerases delta and epsilon showed accumulation of single-strand breaks indicating defective repair. Mutations in other DNA polymerase genes exhibited no defects in DNA repair. Thus, the data obtained suggest that DNA polymerases delta and epsilon are both necessary for DNA replication and for repair of lesions caused by UV irradiation. The results are discussed in the light of current concepts concerning the specificity of DNA polymerases in DNA repair.

Direction of chromosome rearrangements in Saccharomyces cerevisiae by use of his3 recombinational substrates

1988 ◽  
Vol 8 (10) ◽  
pp. 4370-4380
Author(s):  
M T Fasullo ◽  
R W Davis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Chromosomal Dna ◽  
Yeast Saccharomyces Cerevisiae ◽  
Coding Sequences ◽  
Mutant Strains ◽  
Genetic Techniques ◽  
Orthogonal Field ◽  
His3 Gene ◽  
Tandem Duplications

We used the his3 recombinational substrates (his3 fragments) to direct large interchromosomal (translocations) and intrachromosomal (deletions and tandem duplications) rearrangements in the yeast Saccharomyces cerevisiae. In strains completely deleted for the wild-type HIS3 gene, his3 fragments, one containing a deletion of 5' amino acid coding sequences and the other containing a deletion of 3' amino acid coding sequences, were first placed at preselected sites by homologous recombination. His+ revertants that arose via spontaneous mitotic recombination between the two his3 fragments were selected. This strategy was used to direct rearrangements in both RAD52+ and rad52 mutant strains. Translocations occurred in the RAD52+ genetic background and were characterized by orthogonal field alternating gel electrophoresis of yeast chromosomal DNA and by standard genetic techniques. An unexpected translocation was also identified in which HIS3 sequences were amplified. Two types of tandem duplications of the GAL(7, 10, 1) locus were also directed, and one type was not observed in rad52 mutants. Recombination mechanisms are discussed to account for these differences.

scholarly journals Synthesis of Polyhydroxyalkanoate in the Peroxisome of Saccharomyces cerevisiae by Using Intermediates of Fatty Acid β-Oxidation

2001 ◽  
Vol 67 (11) ◽  
pp. 5254-5260 ◽  
Author(s):  
Yves Poirier ◽  
Nadine Erard ◽  
Jean MacDonald-Comber Petétot
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Isocitrate Lyase ◽  
Dry Weight ◽  
Pha Synthase ◽  
Recombinant Yeast ◽  
Medium Chain Length ◽  
The Media

ABSTRACT Medium-chain-length polyhydroxyalkanoates (PHAs) are polyesters having properties of biodegradable thermoplastics and elastomers that are naturally produced by a variety of pseudomonads.Saccharomyces cerevisiae was transformed with thePseudomonas aeruginosa PHAC1 synthase modified for peroxisome targeting by the addition of the carboxyl 34 amino acids from the Brassica napus isocitrate lyase. The PHAC1 gene was put under the control of the promoter of the catalase A gene. PHA synthase expression and PHA accumulation were found in recombinantS. cerevisiae growing in media containing fatty acids. PHA containing even-chain monomers from 6 to 14 carbons was found in recombinant yeast grown on oleic acid, while odd-chain monomers from 5 to 15 carbons were found in PHA from yeast grown on heptadecenoic acid. The maximum amount of PHA accumulated was 0.45% of the dry weight. Transmission electron microscopy of recombinant yeast grown on oleic acid revealed the presence of numerous PHA inclusions found within membrane-bound organelles. Together, these data show that S. cerevisiae expressing a peroxisomal PHA synthase produces PHA in the peroxisome using the 3-hydroxyacyl coenzyme A intermediates of the β-oxidation of fatty acids present in the media. S. cerevisiaecan thus be used as a powerful model system to learn how fatty acid metabolism can be modified in order to synthesize high amounts of PHA in eukaryotes, including plants.

scholarly journals Transcriptomic Response of Saccharomyces cerevisiae during Fermentation under Oleic Acid and Ergosterol Depletion

Fermentation ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 57 ◽  
Author(s):  
Giacomo Zara ◽  
Hennie J. J. van Vuuren ◽  
Ilaria Mannazzu ◽  
Severino Zara ◽  
Marilena Budroni
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleic Acid ◽  
Lipid Fraction ◽  
Membrane Lipid ◽  
Ergosterol Biosynthesis ◽  
Methionine Biosynthesis ◽  
Transcriptomic Response ◽  
Hypoxic Conditions ◽  
Lipid Supplementation ◽  
Time Point

Under anaerobic/hypoxic conditions, Saccharomyces cerevisiae relies on external lipid supplements to modulate membrane lipid fraction in response to different stresses. Here, transcriptomic responses of two S. cerevisiae wine strains were evaluated during hypoxic fermentation of a synthetic must with/without ergosterol and oleic acid supplementation. In the absence of lipids, the two strains, namely EC1118 and M25, showed different behaviour, with M25 significantly decreasing its fermentation rate from the 72 h after inoculum. At this time point, the whole genome transcriptomic analysis revealed common and strain-specific responses to the lack of lipid supplementation. Common responses included the upregulation of the genes involved in ergosterol biosynthesis, as well as the seripauperin and the heat shock protein multigene families. In addition, the upregulation of the aerobic isoforms of genes involved in mitochondrial electron transport is compatible with the previously observed accumulation of reactive oxygen species in the two strains during growth in absence of lipids. Considering the strain-specific responses, M25 downregulated the transcription of genes involved in glucose transport, methionine biosynthesis and of those encoding mannoproteins required for adaptation to low temperatures and hypoxia. The identification of these pathways, which are presumably involved in yeast resistance to stresses, will assist industrial strain selection.

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