scholarly journals Generation of Human Renal Vesicles in Mouse Organ Niche Using Nephron Progenitor Cell Replacement System

Cell Reports ◽  
2020 ◽  
Vol 32 (11) ◽  
pp. 108130
Author(s):  
Toshinari Fujimoto ◽  
Shuichiro Yamanaka ◽  
Susumu Tajiri ◽  
Tsuyoshi Takamura ◽  
Yatsumu Saito ◽  
...  
Keyword(s):  
Progenitor Cell ◽  
Cell Replacement ◽  
Mouse Organ ◽  
Replacement System ◽  
Nephron Progenitor

scholarly journals In vivo regeneration of interspecies chimeric kidneys using a nephron progenitor cell replacement system

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Toshinari Fujimoto ◽  
Shuichiro Yamanaka ◽  
Susumu Tajiri ◽  
Tsuyoshi Takamura ◽  
Yatsumu Saito ◽  
...  
Keyword(s):  
Progenitor Cell ◽  
Cell Replacement ◽  
Replacement System ◽  
Nephron Progenitor

scholarly journals Generation of interspecies limited chimeric nephrons using a conditional nephron progenitor cell replacement system

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
S. Yamanaka ◽  
S. Tajiri ◽  
T. Fujimoto ◽  
K. Matsumoto ◽  
S. Fukunaga ◽  
...  
Keyword(s):  
Progenitor Cell ◽  
Cell Replacement ◽  
Replacement System ◽  
Nephron Progenitor

scholarly journals Kidney‐in‐a‐lymph node: A novel organogenesis assay to model human renal development and test nephron progenitor cell fates

2019 ◽  
Vol 13 (9) ◽  
pp. 1724-1731 ◽  
Author(s):  
Maria Giovanna Francipane ◽  
Bing Han ◽  
Leif Oxburgh ◽  
Sunder Sims‐Lucas ◽  
Zhongwei Li ◽  
...  
Keyword(s):  
Lymph Node ◽  
Progenitor Cell ◽  
Renal Development ◽  
Cell Fates ◽  
Nephron Progenitor

Metabolic programming of nephron progenitor cell fate

2020 ◽  
Author(s):  
Giovane G Tortelote ◽  
Mariel Colón-Leyva ◽  
Zubaida Saifudeen
Keyword(s):  
Cell Fate ◽  
Progenitor Cell ◽  
Metabolic Programming ◽  
Nephron Progenitor

scholarly journals FP044REGENERATION OF INTERSPECIES CHIMERIC KIDNEYS USING TAMOXIFEN-INDUCED NEPHRON PROGENITOR CELL ELIMINATION SYSTEM

2019 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Toshinari Fujimoto ◽  
Shuichiro Yamanaka ◽  
Tsuyoshi Takamura ◽  
Yatsumu Saito ◽  
Susumu Tajiri ◽  
...  
Keyword(s):  
Progenitor Cell ◽  
Nephron Progenitor

scholarly journals BET Proteins Regulate Expression of Osr1 in Early Kidney Development

Biomedicines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1878
Author(s):  
Janina Schreiber ◽  
Nastassia Liaukouskaya ◽  
Lars Fuhrmann ◽  
Alexander-Thomas Hauser ◽  
Manfred Jung ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cell ◽  
Kidney Development ◽  
Gene Activation ◽  
Renal Development ◽  
Prenatal Programming ◽  
Bet Inhibitor ◽  
Bet Inhibitors ◽  
Nephron Progenitor

In utero renal development is subject to maternal metabolic and environmental influences affecting long-term renal function and the risk of developing chronic kidney failure and cardiovascular disease. Epigenetic processes have been implicated in the orchestration of renal development and prenatal programming of nephron number. However, the role of many epigenetic modifiers for kidney development is still unclear. Bromodomain and extra-terminal domain (BET) proteins act as histone acetylation reader molecules and promote gene transcription. BET family members Brd2, Brd3 and Brd4 are expressed in the nephrogenic zone during kidney development. Here, the effect of the BET inhibitor JQ1 on renal development is evaluated. Inhibition of BET proteins via JQ1 leads to reduced growth of metanephric kidney cultures, loss of the nephron progenitor cell population, and premature and disturbed nephron differentiation. Gene expression of key nephron progenitor transcription factor Osr1 is downregulated after 24 h BET inhibition, while Lhx1 and Pax8 expression is increased. Mining of BRD4 ChIP-seq and gene expression data identify Osr1 as a key factor regulated by BRD4-controlled gene activation. Inhibition of BRD4 by BET inhibitor JQ1 leads to downregulation of Osr1, thereby causing a disturbance in the balance of nephron progenitor cell self-renewal and premature differentiation of the nephron, which ultimately leads to kidney hypoplasia and disturbed nephron development. This raises questions about the potential teratogenic effects of BET inhibitors for embryonic development. In summary, our work highlights the role of BET proteins for prenatal programming of nephrogenesis and identifies Osr1 as a potential target of BET proteins.

Gene Editing in 3D Cultured Nephron Progenitor Cell Lines

2019 ◽  
pp. 151-159 ◽  
Author(s):  
Zhongwei Li ◽  
Toshikazu Araoka ◽  
Juan Carlos Izpisua Belmonte
Keyword(s):  
Cell Lines ◽  
Progenitor Cell ◽  
Gene Editing ◽  
Nephron Progenitor
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