scholarly journals Kidney‐in‐a‐lymph node: A novel organogenesis assay to model human renal development and test nephron progenitor cell fates

2019 ◽  
Vol 13 (9) ◽  
pp. 1724-1731 ◽  
Author(s):  
Maria Giovanna Francipane ◽  
Bing Han ◽  
Leif Oxburgh ◽  
Sunder Sims‐Lucas ◽  
Zhongwei Li ◽  
...  
Keyword(s):  
Lymph Node ◽  
Progenitor Cell ◽  
Renal Development ◽  
Cell Fates ◽  
Nephron Progenitor

scholarly journals BET Proteins Regulate Expression of Osr1 in Early Kidney Development

Biomedicines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1878
Author(s):  
Janina Schreiber ◽  
Nastassia Liaukouskaya ◽  
Lars Fuhrmann ◽  
Alexander-Thomas Hauser ◽  
Manfred Jung ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cell ◽  
Kidney Development ◽  
Gene Activation ◽  
Renal Development ◽  
Prenatal Programming ◽  
Bet Inhibitor ◽  
Bet Inhibitors ◽  
Nephron Progenitor

In utero renal development is subject to maternal metabolic and environmental influences affecting long-term renal function and the risk of developing chronic kidney failure and cardiovascular disease. Epigenetic processes have been implicated in the orchestration of renal development and prenatal programming of nephron number. However, the role of many epigenetic modifiers for kidney development is still unclear. Bromodomain and extra-terminal domain (BET) proteins act as histone acetylation reader molecules and promote gene transcription. BET family members Brd2, Brd3 and Brd4 are expressed in the nephrogenic zone during kidney development. Here, the effect of the BET inhibitor JQ1 on renal development is evaluated. Inhibition of BET proteins via JQ1 leads to reduced growth of metanephric kidney cultures, loss of the nephron progenitor cell population, and premature and disturbed nephron differentiation. Gene expression of key nephron progenitor transcription factor Osr1 is downregulated after 24 h BET inhibition, while Lhx1 and Pax8 expression is increased. Mining of BRD4 ChIP-seq and gene expression data identify Osr1 as a key factor regulated by BRD4-controlled gene activation. Inhibition of BRD4 by BET inhibitor JQ1 leads to downregulation of Osr1, thereby causing a disturbance in the balance of nephron progenitor cell self-renewal and premature differentiation of the nephron, which ultimately leads to kidney hypoplasia and disturbed nephron development. This raises questions about the potential teratogenic effects of BET inhibitors for embryonic development. In summary, our work highlights the role of BET proteins for prenatal programming of nephrogenesis and identifies Osr1 as a potential target of BET proteins.

Metabolic programming of nephron progenitor cell fate

2020 ◽  
Author(s):  
Giovane G Tortelote ◽  
Mariel Colón-Leyva ◽  
Zubaida Saifudeen
Keyword(s):  
Cell Fate ◽  
Progenitor Cell ◽  
Metabolic Programming ◽  
Nephron Progenitor

scholarly journals FP044REGENERATION OF INTERSPECIES CHIMERIC KIDNEYS USING TAMOXIFEN-INDUCED NEPHRON PROGENITOR CELL ELIMINATION SYSTEM

2019 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Toshinari Fujimoto ◽  
Shuichiro Yamanaka ◽  
Tsuyoshi Takamura ◽  
Yatsumu Saito ◽  
Susumu Tajiri ◽  
...  
Keyword(s):  
Progenitor Cell ◽  
Nephron Progenitor

scholarly journals Delta-Notch Signaling: The Long and The Short of a Neuron’s Influence on Progenitor Fates

2020 ◽  
Vol 8 (2) ◽  
pp. 8 ◽  
Author(s):  
Rachel Moore ◽  
Paula Alexandre
Keyword(s):  
Progenitor Cell ◽  
Radial Glia ◽  
Notch Signalling ◽  
Neural Progenitors ◽  
Cell Fates ◽  
Basal Surface ◽  
Neuroepithelial Cells ◽  
Mantle Layer ◽  
The Central Nervous System ◽  
Radial Glial

Maintenance of the neural progenitor pool during embryonic development is essential to promote growth of the central nervous system (CNS). The CNS is initially formed by tightly compacted proliferative neuroepithelial cells that later acquire radial glial characteristics and continue to divide at the ventricular (apical) and pial (basal) surface of the neuroepithelium to generate neurons. While neural progenitors such as neuroepithelial cells and apical radial glia form strong connections with their neighbours at the apical and basal surfaces of the neuroepithelium, neurons usually form the mantle layer at the basal surface. This review will discuss the existing evidence that supports a role for neurons, from early stages of differentiation, in promoting progenitor cell fates in the vertebrates CNS, maintaining tissue homeostasis and regulating spatiotemporal patterning of neuronal differentiation through Delta-Notch signalling.

scholarly journals Generation of Human Renal Vesicles in Mouse Organ Niche Using Nephron Progenitor Cell Replacement System

Cell Reports ◽  
2020 ◽  
Vol 32 (11) ◽  
pp. 108130
Author(s):  
Toshinari Fujimoto ◽  
Shuichiro Yamanaka ◽  
Susumu Tajiri ◽  
Tsuyoshi Takamura ◽  
Yatsumu Saito ◽  
...  
Keyword(s):  
Progenitor Cell ◽  
Cell Replacement ◽  
Mouse Organ ◽  
Replacement System ◽  
Nephron Progenitor

scholarly journals Stem/Progenitor Cell Niches Involved in Hepatic and Biliary Regeneration

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Guido Carpino ◽  
Anastasia Renzi ◽  
Antonio Franchitto ◽  
Vincenzo Cardinale ◽  
Paolo Onori ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Bile Ducts ◽  
Progenitor Cell ◽  
Stellate Cells ◽  
Biliary Tree ◽  
Central Vein ◽  
Cell Fates ◽  
Pancreatic Cell ◽  
Intrahepatic Bile Ducts ◽  
Peribiliary Glands

Niches containing stem/progenitor cells are present in different anatomical locations along the human biliary tree and within liver acini. The most primitive stem/progenitors, biliary tree stem/progenitor cells (BTSCs), reside within peribiliary glands located throughout large extrahepatic and intrahepatic bile ducts. BTSCs are multipotent and can differentiate towards hepatic and pancreatic cell fates. These niches’ matrix chemistry and other characteristics are undefined. Canals of Hering (bile ductules) are found periportally and contain hepatic stem/progenitor cells (HpSCs), participating in the renewal of small intrahepatic bile ducts and being precursors to hepatocytes and cholangiocytes. The niches also contain precursors to hepatic stellate cells and endothelia, macrophages, and have a matrix chemistry rich in hyaluronans, minimally sulfated proteoglycans, fetal collagens, and laminin. The microenvironment furnishes key signals driving HpSC activation and differentiation. Newly discovered third niches are pericentral within hepatic acini, contain Axin2+ unipotent hepatocytic progenitors linked on their lateral borders to endothelia forming the central vein, and contribute to normal turnover of mature hepatocytes. Their relationship to the other stem/progenitors is undefined. Stem/progenitor niches have important implications in regenerative medicine for the liver and biliary tree and in pathogenic processes leading to diseases of these tissues.

scholarly journals Computational prediction of neural progenitor cell fates

2010 ◽  
Vol 7 (3) ◽  
pp. 213-218 ◽  
Author(s):  
Andrew R Cohen ◽  
Francisco L A F Gomes ◽  
Badrinath Roysam ◽  
Michel Cayouette
Keyword(s):  
Progenitor Cell ◽  
Computational Prediction ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Cell Fates

scholarly journals Analyzing signaling activity and function in hematopoietic cells

2021 ◽  
Vol 218 (7) ◽  
Author(s):  
Tobias Kull ◽  
Timm Schroeder
Keyword(s):  
Cell Signaling ◽  
Signaling Pathways ◽  
Progenitor Cell ◽  
Cell Behavior ◽  
Cell Fates ◽  
Hematopoietic Stem ◽  
Related Individual ◽  
Health And Disease ◽  
And Function ◽  
Signaling Activity

Cells constantly sense their environment, allowing the adaption of cell behavior to changing needs. Fine-tuned responses to complex inputs are computed by signaling pathways, which are wired in complex connected networks. Their activity is highly context-dependent, dynamic, and heterogeneous even between closely related individual cells. Despite lots of progress, our understanding of the precise implementation, relevance, and possible manipulation of cellular signaling in health and disease therefore remains limited. Here, we discuss the requirements, potential, and limitations of the different current technologies for the analysis of hematopoietic stem and progenitor cell signaling and its effect on cell fates.

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