scholarly journals A Non-Replicative Role of the 3′ Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation

Cell Reports ◽  
2020 ◽  
Vol 32 (1) ◽  
pp. 107859 ◽  
Author(s):  
Berati Cerikan ◽  
Sarah Goellner ◽  
Christopher John Neufeldt ◽  
Uta Haselmann ◽  
Klaas Mulder ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Genome ◽  
Terminal Sequence

scholarly journals Intra-Colonial Viral Infections in Western Honey Bees (Apis mellifera)

2021 ◽  
Vol 9 (5) ◽  
pp. 1087
Author(s):  
Loreley Castelli ◽  
María Laura Genchi García ◽  
Anne Dalmon ◽  
Daniela Arredondo ◽  
Karina Antúnez ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Viral Infections ◽  
Sampling Period ◽  
Virus Genome ◽  
The Individual ◽  
Bee Virus ◽  
Insight Into ◽  
Bee Viruses

RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.

scholarly journals Antiviral Role of Phenolic Compounds against Dengue Virus: A Review

Biomolecules ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Vanessa Loaiza-Cano ◽  
Laura Milena Monsalve-Escudero ◽  
Carlos da Silva Maia Bezerra Filho ◽  
Marlen Martinez-Gutierrez ◽  
Damião Pergentino de Sousa
Keyword(s):  
Phenolic Compounds ◽  
Dengue Virus ◽  
Biological Activities ◽  
Antiviral Effect ◽  
Health Concern ◽  
Host Proteins ◽  
Viral Particles ◽  
Antiviral Role ◽  
Productive Replication

Phenolic compounds have been related to multiple biological activities, and the antiviral effect of these compounds has been demonstrated in several viral models of public health concern. In this review, we show the antiviral role of phenolic compounds against dengue virus (DENV), the most widespread arbovirus globally that, after its re-emergence, has caused multiple epidemic outbreaks, especially in the last two years. Twenty phenolic compounds with anti-DENV activity are discussed, including the multiple mechanisms of action, such as those directed against viral particles or viral proteins, host proteins or pathways related to the productive replication viral cycle and the spread of the infection.

scholarly journals Role of the CCA terminal sequence of tRNA(Val) in aminoacylation with valyl-tRNA synthetase.

1994 ◽  
Vol 269 (35) ◽  
pp. 22173-22177 ◽  
Author(s):  
K. Tamura ◽  
N. Nameki ◽  
T. Hasegawa ◽  
M. Shimizu ◽  
H. Himeno
Keyword(s):  
Trna Synthetase ◽  
Terminal Sequence

scholarly journals The possible role of cross-reactive dengue virus antibodies in Zika virus pathogenesis

2019 ◽  
Vol 15 (4) ◽  
pp. e1007640 ◽  
Author(s):  
Thomas Langerak ◽  
Noreen Mumtaz ◽  
Vera I. Tolk ◽  
Eric C. M. van Gorp ◽  
Byron E. Martina ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Zika Virus

Role of polarization in ligand docking and binding affinity prediction for inhibitors of dengue virus

2011 ◽  
Vol 21 (7) ◽  
pp. 1030-1038 ◽  
Author(s):  
Kshatresh Dutta Dubey ◽  
Amit Kumar Chaubey ◽  
Rajendra Prasad Ojha
Keyword(s):  
Dengue Virus ◽  
Binding Affinity ◽  
Ligand Docking ◽  
Binding Affinity Prediction ◽  
Affinity Prediction

scholarly journals SIR Model for Dengue Disease with Effect of Dengue Vaccination

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Pratchaya Chanprasopchai ◽  
I. Ming Tang ◽  
Puntani Pongsumpun
Keyword(s):  
Mathematical Model ◽  
Mortality Rate ◽  
Dengue Virus ◽  
Numerical Solutions ◽  
Specific Treatment ◽  
Sir Model ◽  
Dengue Vaccine ◽  
Dengue Disease ◽  
Modelling Methods

The dengue disease is caused by dengue virus, and there is no specific treatment. The medical care by experienced physicians and nurses will save life and will lower the mortality rate. A dengue vaccine to control the disease is available in Thailand since late 2016. A mathematical model would be an important way to analyze the effects of the vaccination on the transmission of the disease. We have formulated an SIR (susceptible-infected-recovered) model of the transmission of the disease which includes the effect of vaccination and used standard dynamical modelling methods to analyze the effects. The equilibrium states and their stabilities are investigated. The trajectories of the numerical solutions plotted into the 2D planes and 3D spaces are presented. The main contribution is determining the role of dengue vaccination in the model. From the analysis, we find that there is a significant reduction in the total hospitalization time needed to treat the illness.

scholarly journals Biochemical and genetic analysis of the role of the viral polymerase in enterovirus recombination

2016 ◽  
Vol 44 (14) ◽  
pp. 6883-6895 ◽  
Author(s):  
Andrew Woodman ◽  
Jamie J. Arnold ◽  
Craig E. Cameron ◽  
David J. Evans
Keyword(s):  
Genetic Recombination ◽  
Evolutionary Process ◽  
Virus Genome ◽  
Biochemical Assay ◽  
Strand Transfer ◽  
Turnover Rates ◽  
Coding Region ◽  
Cis Acting ◽  
Polymerase Fidelity

Abstract Genetic recombination in single-strand, positive-sense RNA viruses is a poorly understand mechanism responsible for generating extensive genetic change and novel phenotypes. By moving a critical cis-acting replication element (CRE) from the polyprotein coding region to the 3′ non-coding region we have further developed a cell-based assay (the 3′CRE-REP assay) to yield recombinants throughout the non-structural coding region of poliovirus from dually transfected cells. We have additionally developed a defined biochemical assay in which the only protein present is the poliovirus RNA dependent RNA polymerase (RdRp), which recapitulates the strand transfer events of the recombination process. We have used both assays to investigate the role of the polymerase fidelity and nucleotide turnover rates in recombination. Our results, of both poliovirus intertypic and intratypic recombination in the CRE-REP assay and using a range of polymerase variants in the biochemical assay, demonstrate that RdRp fidelity is a fundamental determinant of recombination frequency. High fidelity polymerases exhibit reduced recombination and low fidelity polymerases exhibit increased recombination in both assays. These studies provide the basis for the analysis of poliovirus recombination throughout the non-structural region of the virus genome and provide a defined biochemical assay to further dissect this important evolutionary process.

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