scholarly journals Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles

Cell Reports ◽  
2020 ◽  
Vol 31 (11) ◽  
pp. 107756 ◽  
Author(s):  
Iliana Siamishi ◽  
Norimasa Iwanami ◽  
Thomas Clapes ◽  
Eirini Trompouki ◽  
Connor P. O’Meara ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Specific Function ◽  
Dna Polymerase Epsilon ◽  
Epsilon Subunit ◽  
Polymerase Epsilon

Effects of the anticancer drug cis-diamminedichloroplatinum(II) on the activities of calf thymus DNA polymerase .epsilon.

Biochemistry ◽  
1993 ◽  
Vol 32 (3) ◽  
pp. 841-848 ◽  
Author(s):  
Lin Huang ◽  
John J. Turchi ◽  
Alan F. Wahl ◽  
Robert A. Bambara
Keyword(s):  
Dna Polymerase ◽  
Anticancer Drug ◽  
Calf Thymus Dna ◽  
Dna Polymerase Epsilon ◽  
Calf Thymus ◽  
Polymerase Epsilon

scholarly journals Analysis of APOBEC-induced mutations in yeast strains with low levels of replicative DNA polymerases

2020 ◽  
Vol 117 (17) ◽  
pp. 9440-9450 ◽  
Author(s):  
Yang Sui ◽  
Lei Qi ◽  
Ke Zhang ◽  
Natalie Saini ◽  
Leszek J. Klimczak ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerases ◽  
Induced Mutations ◽  
Single Stranded Dna ◽  
Dna Polymerase Epsilon ◽  
Dna Polymerase Alpha ◽  
Yeast Strains ◽  
Polymerase Epsilon ◽  
Low Levels ◽  
Lagging Strand

Yeast strains with low levels of the replicative DNA polymerases (alpha, delta, and epsilon) have high levels of chromosome deletions, duplications, and translocations. By examining the patterns of mutations induced in strains with low levels of DNA polymerase by the human protein APOBEC3B (a protein that deaminates cytosine in single-stranded DNA), we show dramatically elevated amounts of single-stranded DNA relative to a wild-type strain. During DNA replication, one strand (defined as the leading strand) is replicated processively by DNA polymerase epsilon and the other (the lagging strand) is replicated as short fragments initiated by DNA polymerase alpha and extended by DNA polymerase delta. In the low DNA polymerase alpha and delta strains, the APOBEC-induced mutations are concentrated on the lagging-strand template, whereas in the low DNA polymerase epsilon strain, mutations occur on the leading- and lagging-strand templates with similar frequencies. In addition, for most genes, the transcribed strand is mutagenized more frequently than the nontranscribed strand. Lastly, some of the APOBEC-induced clusters in strains with low levels of DNA polymerase alpha or delta are greater than 10 kb in length.

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