scholarly journals Cell Cycle-dependent Phosphorylation of the DNA Polymerase Epsilon Subunit, Dpb2, by the Cdc28 Cyclin-dependent Protein Kinase

2004 ◽  
Vol 279 (14) ◽  
pp. 14245-14255 ◽  
Author(s):  
Tapio Kesti ◽  
W. Hayes McDonald ◽  
John R. Yates ◽  
Curt Wittenberg
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Dna Polymerase ◽  
Dependent Protein Kinase ◽  
Dna Polymerase Epsilon ◽  
Epsilon Subunit ◽  
Dependent Protein ◽  
Polymerase Epsilon ◽  
Cycle Dependent

scholarly journals Cell cycle-dependent regulation of the DNA-dependent protein kinase

1999 ◽  
Vol 32 (4) ◽  
pp. 239-248 ◽  
Author(s):  
A. Nilsson ◽  
F. Sirzén ◽  
R. Lewensohn ◽  
N. Wang ◽  
S. Skog
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Cycle Dependent

scholarly journals The Saccharomyces cerevisiae YAK1 gene encodes a protein kinase that is induced by arrest early in the cell cycle.

1991 ◽  
Vol 11 (8) ◽  
pp. 4045-4052 ◽  
Author(s):  
S Garrett ◽  
M M Menold ◽  
J R Broach
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Kinase Activity ◽  
Specific Activity ◽  
S Phase ◽  
Protein Kinase Activity ◽  
Dependent Protein Kinase ◽  
Protein Levels ◽  
Cycle Arrest ◽  
Dependent Protein

Null mutations in the gene YAK1, which encodes a protein with sequence homology to known protein kinases, suppress the cell cycle arrest phenotype of mutants lacking the cyclic AMP-dependent protein kinase (A kinase). That is, loss of the YAK1 protein specifically compensates for loss of the A kinase. Here, we show that the protein encoded by YAK1 has protein kinase activity. Yak1 kinase activity is low during exponential growth but is induced at least 50-fold by arrest of cells prior to the completion of S phase. Induction is not observed by arrest at stages later in the cell cycle. Depending on the arrest regimen, induction can occur either by an increase in Yak1 protein levels or by an increase in Yak1 specific activity. Finally, an increase in Yak1 protein levels causes growth arrest of cells with attenuated A kinase activity. These results suggest that Yak1 acts in a pathway parallel to that of the A kinase to negatively regulate cell proliferation.

scholarly journals Xenopus Cds1 Is Regulated by DNA-Dependent Protein Kinase and ATR during the Cell Cycle Checkpoint Response to Double-Stranded DNA Ends

2004 ◽  
Vol 24 (22) ◽  
pp. 9968-9985 ◽  
Author(s):  
Troy D. McSherry ◽  
Paul R. Mueller
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Cell Cycle Checkpoint ◽  
Dual Function ◽  
Dependent Protein Kinase ◽  
Double Stranded Dna ◽  
Cycle Checkpoint ◽  
Protein Dissociation ◽  
Dependent Protein ◽  
Dna Ends

ABSTRACT The checkpoint kinase Cds1 (Chk2) plays a key role in cell cycle checkpoint responses with functions in cell cycle arrest, DNA repair, and induction of apoptosis. Proper regulation of Cds1 is essential for appropriate cellular responses to checkpoint-inducing insults. While the kinase ATM has been shown to be important in the regulation of human Cds1 (hCds1), here we report that the kinases ATR and DNA-dependent protein kinase (DNA-PK) play more significant roles in the regulation of Xenopus Cds1 (XCds1). Under normal cell cycle conditions, nonactivated XCds1 constitutively associates with a Xenopus ATR complex. The association of XCds1 with this complex does not require a functional forkhead activation domain but does require a putative SH3 binding region that is found in XCds1. In response to double-stranded DNA ends, the amino terminus of XCds1 is rapidly phosphorylated in a sequential pattern. First DNA-PK phosphorylates serine 39, a site not previously recognized as important in Cds1 regulation. Xenopus ATM, ATR, and/or DNA-PK then phosphorylate three consensus serine/glutamine sites. Together, these phosphorylations have the dual function of inducing dissociation from the ATR complex and independently promoting the full activation of XCds1. Thus, the checkpoint-mediated activation of XCds1 requires phosphorylation by multiple phosphoinositide 3-kinase-related kinases, protein-protein dissociation, and autophosphorylation.

scholarly journals Ras/cAMP-dependent Protein Kinase (PKA) Regulates Multiple Aspects of Cellular Events by Phosphorylating the Whi3 Cell Cycle Regulator in Budding Yeast

2013 ◽  
Vol 288 (15) ◽  
pp. 10558-10566 ◽  
Author(s):  
Masaki Mizunuma ◽  
Ryohei Tsubakiyama ◽  
Takafumi Ogawa ◽  
Atsunori Shitamukai ◽  
Yoshifumi Kobayashi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Budding Yeast ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Cellular Events ◽  
Cell Cycle Regulator ◽  
Dependent Protein

scholarly journals Involvement of DNA-dependent Protein Kinase in Normal Cell Cycle Progression through Mitosis

2011 ◽  
Vol 286 (14) ◽  
pp. 12796-12802 ◽  
Author(s):  
Kyung-Jong Lee ◽  
Yu-Fen Lin ◽  
Han-Yi Chou ◽  
Hirohiko Yajima ◽  
Kazi R. Fattah ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Normal Cell ◽  
Cell Cycle Progression ◽  
Dependent Protein Kinase ◽  
Cycle Progression ◽  
Dependent Protein ◽  
Normal Cell Cycle
Sign in / Sign up

Export Citation Format

Share Document