scholarly journals Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca2+/CaM Controls DCV Binding and Liprin-α/TANC2 Recruits DCVs to Postsynaptic Sites

Cell Reports ◽  
2018 ◽  
Vol 24 (3) ◽  
pp. 685-700 ◽  
Author(s):  
Riccardo Stucchi ◽  
Gabriela Plucińska ◽  
Jessica J.A. Hummel ◽  
Eitan E. Zahavi ◽  
Irune Guerra San Juan ◽  
...  
Keyword(s):  
Vesicle Transport ◽  
Dense Core ◽  
Dense Core Vesicle

scholarly journals Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons

2021 ◽  
Vol 2 (1) ◽  
pp. 100325
Author(s):  
Alessandro Moro ◽  
Rein I. Hoogstraaten ◽  
Claudia M. Persoon ◽  
Matthijs Verhage ◽  
Ruud F. Toonen
Keyword(s):  
Quantitative Analysis ◽  
Vesicle Fusion ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Cns Neurons

scholarly journals The Conserved VPS-50 Protein Functions in Dense-Core Vesicle Maturation and Acidification and Controls Animal Behavior

2016 ◽  
Vol 26 (7) ◽  
pp. 862-871 ◽  
Author(s):  
Nicolas Paquin ◽  
Yasunobu Murata ◽  
Allan Froehlich ◽  
Daniel T. Omura ◽  
Michael Ailion ◽  
...  
Keyword(s):  
Animal Behavior ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Protein Functions

scholarly journals The Phosphoprotein Synapsin Ia Regulates the Kinetics of Dense-Core Vesicle Release

2021 ◽  
Vol 41 (13) ◽  
pp. 2828-2841
Author(s):  
Hui-Ju Yang ◽  
Pin-Chun Chen ◽  
Chien-Ting Huang ◽  
Tzu-Lin Cheng ◽  
Sheng-Ping Hsu ◽  
...  
Keyword(s):  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Release ◽  
Kinetics Of

scholarly journals Role of the polybasic sequence in the Doc2α C2B domain in dense-core vesicle exocytosis in PC12 cells

2010 ◽  
pp. no-no ◽  
Author(s):  
Mai Sato ◽  
Yasunori Mori ◽  
Takahide Matsui ◽  
Ryo Aoki ◽  
Manami Oya ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals BAIAP3, a C2 domain–containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells

2017 ◽  
Vol 216 (7) ◽  
pp. 2151-2166 ◽  
Author(s):  
Xingmin Zhang ◽  
Shan Jiang ◽  
Kelly A. Mitok ◽  
Lingjun Li ◽  
Alan D. Attie ◽  
...  
Keyword(s):  
Neuroendocrine Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Protein Homeostasis ◽  
C2 Domain ◽  
Β Cells ◽  
Calcium Dependent ◽  
Trafficking Pathway ◽  
Protein Receptor ◽  
Golgi Network

Dense-core vesicle (DCV) exocytosis is a SNARE (soluble N-ethylmaleimide–sensitive fusion attachment protein receptor)-dependent anterograde trafficking pathway that requires multiple proteins for regulation. Several C2 domain–containing proteins are known to regulate Ca2+-dependent DCV exocytosis in neuroendocrine cells. In this study, we identified others by screening all (∼139) human C2 domain–containing proteins by RNA interference in neuroendocrine cells. 40 genes were identified, including several encoding proteins with known roles (CAPS [calcium-dependent activator protein for secretion 1], Munc13-2, RIM1, and SYT10) and many with unknown roles. One of the latter, BAIAP3, is a secretory cell–specific Munc13-4 paralog of unknown function. BAIAP3 knockdown caused accumulation of fusion-incompetent DCVs in BON neuroendocrine cells and lysosomal degradation (crinophagy) of insulin-containing DCVs in INS-1 β cells. BAIAP3 localized to endosomes was required for Golgi trans-Golgi network 46 (TGN46) recycling, exhibited Ca2+-stimulated interactions with TGN SNAREs, and underwent Ca2+-stimulated TGN recruitment. Thus, unlike other Munc13 proteins, BAIAP3 functions indirectly in DCV exocytosis by affecting DCV maturation through its role in DCV protein recycling. Ca2+ rises that stimulate DCV exocytosis may stimulate BAIAP3-dependent retrograde trafficking to maintain DCV protein homeostasis and DCV function.

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