Neuronal CTCF Is Necessary for Basal and Experience-Dependent Gene Regulation, Memory Formation, and Genomic Structure of BDNF and Arc

Dev Sharan Sams; Stefano Nardone; Dmitriy Getselter; Dana Raz; Moran Tal; Prudhvi Raj Rayi; Hanoch Kaphzan; Ofir Hakim; Evan Elliott

doi:10.1016/j.celrep.2016.11.004

Neuronal CTCF Is Necessary for Basal and Experience-Dependent Gene Regulation, Memory Formation, and Genomic Structure of BDNF and Arc

Cell Reports ◽

10.1016/j.celrep.2016.11.004 ◽

2016 ◽

Vol 17 (9) ◽

pp. 2418-2430 ◽

Cited By ~ 32

Author(s):

Dev Sharan Sams ◽

Stefano Nardone ◽

Dmitriy Getselter ◽

Dana Raz ◽

Moran Tal ◽

...

Keyword(s):

Gene Regulation ◽

Genomic Structure ◽

Memory Formation

Download Full-text

Dynamical Mechanisms for Gene Regulation Mediated by Two Noncoding RNAs in Long-Term Memory Formation

Neural Plasticity ◽

10.1155/2021/6668389 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Lijie Hao ◽

Zhuoqin Yang

Keyword(s):

Gene Expression ◽

Gene Regulation ◽

Regulation Of Gene Expression ◽

Network Motif ◽

Noncoding Rnas ◽

Memory Formation ◽

Long Term Memory ◽

Term Memory ◽

Motif Model

Noncoding RNAs such as miRNAs and piRNAs have long-lasting effects on the regulation of gene expression involved in long-term synaptic changes. To characterize gene regulation mediated by small noncoding RNAs associated with long-term memory in Aplysia, we consider two noncoding RNAs stimulated by 5-HT into a gene regulatory network motif model, including miR-124 that binds to and inhibits the mRNA of CREB1 and piR-F that facilitates serotonin-dependent DNA methylation to lead to repression of CREB2. Codimension-1 and -2 bifurcation analyses of 5-HT regulating both miR-124 and piR-F and a negative feedback strength for oscillation reveal rich dynamical properties of bistability and oscillations robust to variations in all other parameters. More importantly, we verify three stimulus protocols of 5-HT in experiments by our model and find that application of five pulses of 5-HT leads to a transient decrease of miR-124 but increase of piR-F concentrations, which matters sustained high level of CREB1 concentration associated with long-term memory. Furthermore, we perform bifurcation analyses for the concentrations of miR-124 and piR-F as two parameters to explore dynamical mechanisms underlying the epigenetic regulation in long-term memory formation. This study provides insights into revealing regulatory roles of epigenetic changes in gene expression involving noncoding RNAs associated with synaptic plasticity.

Download Full-text