Modulation of Nrf2 expression alters high glucose-induced oxidative stress and antioxidant gene expression in mouse mesangial cells

2011 ◽  
Vol 23 (10) ◽  
pp. 1625-1632 ◽  
Author(s):  
Hang Li ◽  
Fuxu Wang ◽  
Lianshan Zhang ◽  
Yanping Cao ◽  
Wei Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
High Glucose ◽  
Mesangial Cells ◽  
Antioxidant Gene ◽  
Antioxidant Gene Expression ◽  
Nrf2 Expression

An analysis of high glucose and glucosamine-induced gene expression and oxidative stress in renal mesangial cells

2006 ◽  
Vol 112 (4-5) ◽  
pp. 189-218 ◽  
Author(s):  
Davis W. Cheng ◽  
Yan Jiang ◽  
Anath Shalev ◽  
Renu Kowluru ◽  
Errol D. Crook ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
High Glucose ◽  
Mesangial Cells ◽  
And Oxidative Stress

scholarly journals In response to oxidative stress, the expression of inflammatory cytokines and antioxidant enzymes are impaired in placenta, but not adipose tissue, of women with gestational diabetes

2009 ◽  
Vol 204 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Martha Lappas ◽  
Amberlee Mittion ◽  
Michael Permezel
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Adipose Tissue ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Mrna Expression ◽  
Human Placenta ◽  
Cytokine Release ◽  
Antioxidant Gene ◽  
Antioxidant Gene Expression

In response to oxidative stress, gestational diabetes mellitus (GDM) placenta releases less 8-isoprostane and tumour necrosis factor (TNF) α. The effect of oxidative stress on other cytokines and antioxidant gene expressions are unknown. The aim of this study is to further explore the antioxidant status and effect of oxidative stress in GDM tissue. Human placenta, omental and subcutaneous adipose tissue from women with and without GDM were exposed to hypoxanthine (HX)/xanthine oxidase (XO). Cytokine release was analysed by ELISA and cytokine and antioxidant gene expression by RT-PCR. Catalase (CAT) and glutathione reductase (GSR) mRNA expression was higher in GDM (n=18) compared with normal (n=23) placenta. There was no difference in glutathione peroxidase and superoxide dismutase mRNA expression. Antioxidant gene expression was unaltered between normal (n=18) and GDM (n=10) adipose tissue. HX/XO treatment significantly stimulated cytokine release (13/16 cytokines) and cytokine mRNA expression, and decreased antioxidant gene expression (CAT and GSR) in human placenta from normal pregnant women. In GDM placenta, HX/XO only significantly increased the release of 3/16 cytokines, while there was no effect on antioxidant gene expression. In normal and GDM adipose tissues, HX/XO increased proinflammatory cytokine and 8-isoprostane release, while there was no change in antioxidant gene expression. GDM placenta is characterised by increased antioxidant gene expression, and is less responsive to exogenous oxidative stress than tissues obtained from normal pregnant women. This may represent a protective or adaptive mechanism to prevent damage from further oxidative insult in utero as indicated by increased tissue antioxidant expression.

scholarly journals The Antitumor Agent Imexon Activates Antioxidant Gene Expression: Evidence for an Oxidative Stress Response

2007 ◽  
Vol 13 (11) ◽  
pp. 3388-3394 ◽  
Author(s):  
Amanda F. Baker ◽  
Terry Landowski ◽  
Robert Dorr ◽  
Wendy R. Tate ◽  
Jaime M.C. Gard ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Stress Response ◽  
Antitumor Agent ◽  
Oxidative Stress Response ◽  
Antioxidant Gene ◽  
Antioxidant Gene Expression ◽  
Expression Evidence

scholarly journals BRCA1 Induces Antioxidant Gene Expression and Resistance to Oxidative Stress

2004 ◽  
Vol 64 (21) ◽  
pp. 7893-7909 ◽  
Author(s):  
Insoo Bae ◽  
Saijun Fan ◽  
Qinghui Meng ◽  
Jeong Keun Rih ◽  
Hee Jong Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Antioxidant Gene ◽  
Antioxidant Gene Expression

scholarly journals AGE-Rich Bread Crust Extract Boosts Oxidative Stress Interception via Stimulation of the NRF2 Pathway

Nutrients ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 3874
Author(s):  
Kristin Wächter ◽  
Alexander Navarrete Santos ◽  
Anne Großkopf ◽  
Tim Baldensperger ◽  
Marcus A. Glomb ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Antioxidant Gene ◽  
Nrf2 Pathway ◽  
Cellular Models ◽  
Nrf2 Signaling Pathway ◽  
Bread Crust ◽  
Antioxidant Gene Expression

Advanced glycation end products (AGEs) result from a non-enzymatic reaction of proteins with reactive carbohydrates. Heat-processed food, such as bread, contains high amounts of AGEs. The activation of the NF-κB signaling pathway by bread crust extract (BCE) is well understood. However, it is largely unknown whether NRF2, the master regulator of oxidative stress resistance in mammalian cells, is affected by BCE. We have investigated the molecular mechanisms by which BCE induces antioxidant gene expression in cellular models. Our data showed that soluble extracts from bread crust are capable of stimulating the NRF2 signaling pathway. Furthermore, NRF2 pathway activation was confirmed by microarray and reporter-cell analyses. QRT-PCR measurements and Western blot analyses indicated an induction of antioxidative genes such as HMOX1, GCLM and NQO1 upon BCE treatment. Moreover, BCE pretreated cells had a survival advantage compared to control cells when exposed to oxidative stress. BCE induces phosphorylation of AKT and ERK kinase in EA.hy926 cells. By mass spectrometry, several new, potentially active modifications in BCE were identified. Our findings indicate that BCE activates NRF2-dependent antioxidant gene expression, thus provoking a protection mechanism against oxidative stress-mediated tissue injury. Hence, BCE can be considered as functional food with antioxidative and cardioprotective potential.

scholarly journals Mitochondrial injury, oxidative stress, and antioxidant gene expression are induced by hepatitis C virus core protein

2002 ◽  
Vol 122 (2) ◽  
pp. 366-375 ◽  
Author(s):  
Michiari Okuda ◽  
Kui Li ◽  
Michael R. Beard ◽  
Lori A. Showalter ◽  
Frank Scholle ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Antioxidant Gene ◽  
Mitochondrial Injury ◽  
Virus Core ◽  
Antioxidant Gene Expression
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