Hyperglycemia, hypoxia and their combination exert oxidative stress and changes in antioxidant gene expression: studies on cultured rat embryos

2011 ◽  
Vol 92 (3) ◽  
pp. 231-239 ◽  
Author(s):  
Asher Ornoy ◽  
Alicia Livshitz ◽  
Zivanit Ergaz ◽  
Chais J. Stodgell ◽  
Richard K. Miller
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Antioxidant Gene ◽  
Rat Embryos ◽  
Expression Studies ◽  
Antioxidant Gene Expression ◽  
Gene Expression Studies

scholarly journals In response to oxidative stress, the expression of inflammatory cytokines and antioxidant enzymes are impaired in placenta, but not adipose tissue, of women with gestational diabetes

2009 ◽  
Vol 204 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Martha Lappas ◽  
Amberlee Mittion ◽  
Michael Permezel
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Adipose Tissue ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Mrna Expression ◽  
Human Placenta ◽  
Cytokine Release ◽  
Antioxidant Gene ◽  
Antioxidant Gene Expression

In response to oxidative stress, gestational diabetes mellitus (GDM) placenta releases less 8-isoprostane and tumour necrosis factor (TNF) α. The effect of oxidative stress on other cytokines and antioxidant gene expressions are unknown. The aim of this study is to further explore the antioxidant status and effect of oxidative stress in GDM tissue. Human placenta, omental and subcutaneous adipose tissue from women with and without GDM were exposed to hypoxanthine (HX)/xanthine oxidase (XO). Cytokine release was analysed by ELISA and cytokine and antioxidant gene expression by RT-PCR. Catalase (CAT) and glutathione reductase (GSR) mRNA expression was higher in GDM (n=18) compared with normal (n=23) placenta. There was no difference in glutathione peroxidase and superoxide dismutase mRNA expression. Antioxidant gene expression was unaltered between normal (n=18) and GDM (n=10) adipose tissue. HX/XO treatment significantly stimulated cytokine release (13/16 cytokines) and cytokine mRNA expression, and decreased antioxidant gene expression (CAT and GSR) in human placenta from normal pregnant women. In GDM placenta, HX/XO only significantly increased the release of 3/16 cytokines, while there was no effect on antioxidant gene expression. In normal and GDM adipose tissues, HX/XO increased proinflammatory cytokine and 8-isoprostane release, while there was no change in antioxidant gene expression. GDM placenta is characterised by increased antioxidant gene expression, and is less responsive to exogenous oxidative stress than tissues obtained from normal pregnant women. This may represent a protective or adaptive mechanism to prevent damage from further oxidative insult in utero as indicated by increased tissue antioxidant expression.

scholarly journals The Antitumor Agent Imexon Activates Antioxidant Gene Expression: Evidence for an Oxidative Stress Response

2007 ◽  
Vol 13 (11) ◽  
pp. 3388-3394 ◽  
Author(s):  
Amanda F. Baker ◽  
Terry Landowski ◽  
Robert Dorr ◽  
Wendy R. Tate ◽  
Jaime M.C. Gard ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Stress Response ◽  
Antitumor Agent ◽  
Oxidative Stress Response ◽  
Antioxidant Gene ◽  
Antioxidant Gene Expression ◽  
Expression Evidence

scholarly journals BRCA1 Induces Antioxidant Gene Expression and Resistance to Oxidative Stress

2004 ◽  
Vol 64 (21) ◽  
pp. 7893-7909 ◽  
Author(s):  
Insoo Bae ◽  
Saijun Fan ◽  
Qinghui Meng ◽  
Jeong Keun Rih ◽  
Hee Jong Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Antioxidant Gene ◽  
Antioxidant Gene Expression

scholarly journals AGE-Rich Bread Crust Extract Boosts Oxidative Stress Interception via Stimulation of the NRF2 Pathway

Nutrients ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 3874
Author(s):  
Kristin Wächter ◽  
Alexander Navarrete Santos ◽  
Anne Großkopf ◽  
Tim Baldensperger ◽  
Marcus A. Glomb ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Antioxidant Gene ◽  
Nrf2 Pathway ◽  
Cellular Models ◽  
Nrf2 Signaling Pathway ◽  
Bread Crust ◽  
Antioxidant Gene Expression

Advanced glycation end products (AGEs) result from a non-enzymatic reaction of proteins with reactive carbohydrates. Heat-processed food, such as bread, contains high amounts of AGEs. The activation of the NF-κB signaling pathway by bread crust extract (BCE) is well understood. However, it is largely unknown whether NRF2, the master regulator of oxidative stress resistance in mammalian cells, is affected by BCE. We have investigated the molecular mechanisms by which BCE induces antioxidant gene expression in cellular models. Our data showed that soluble extracts from bread crust are capable of stimulating the NRF2 signaling pathway. Furthermore, NRF2 pathway activation was confirmed by microarray and reporter-cell analyses. QRT-PCR measurements and Western blot analyses indicated an induction of antioxidative genes such as HMOX1, GCLM and NQO1 upon BCE treatment. Moreover, BCE pretreated cells had a survival advantage compared to control cells when exposed to oxidative stress. BCE induces phosphorylation of AKT and ERK kinase in EA.hy926 cells. By mass spectrometry, several new, potentially active modifications in BCE were identified. Our findings indicate that BCE activates NRF2-dependent antioxidant gene expression, thus provoking a protection mechanism against oxidative stress-mediated tissue injury. Hence, BCE can be considered as functional food with antioxidative and cardioprotective potential.

scholarly journals Mitochondrial injury, oxidative stress, and antioxidant gene expression are induced by hepatitis C virus core protein

2002 ◽  
Vol 122 (2) ◽  
pp. 366-375 ◽  
Author(s):  
Michiari Okuda ◽  
Kui Li ◽  
Michael R. Beard ◽  
Lori A. Showalter ◽  
Frank Scholle ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Antioxidant Gene ◽  
Mitochondrial Injury ◽  
Virus Core ◽  
Antioxidant Gene Expression

scholarly journals Resveratrol Attenuates Oxidative Stress-Induced Intestinal Barrier Injury through PI3K/Akt-Mediated Nrf2 Signaling Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Yu Zhuang ◽  
Huirong Wu ◽  
Xiangxiang Wang ◽  
Jieyu He ◽  
Shanping He ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Signaling Pathway ◽  
Intestinal Damage ◽  
Dependent Manner ◽  
Antioxidant Gene ◽  
Expression Levels ◽  
Protein Levels ◽  
Nrf2 Signaling Pathway ◽  
Antioxidant Gene Expression

Oxidative stress is implicated in a wide range of intestinal disorders and closely associated with their pathological processes. Resveratrol (RSV), a plant extract, plays a vital role in protecting various organs in vitro and in vivo. However, the benefits of RSV are controversial, and underlying mechanisms for its antioxidant effects on intestinal epithelial cells remain unclear. In this study, we evaluated the effects of RSV on oxidative stress induced by H2O2 in IPEC-J2 cells. We found that pretreatment with RSV significantly increased cell viability; increased expression levels of tight junction (TJ) proteins (claudin-1, occludin, and ZO-1); improved activities of superoxide dismutase-1 (SOD-1), catalase (CAT), and glutathione peroxidase (GSH-Px); and decreased intracellular reactive oxygen species (ROS) levels and apoptosis induced by H2O2 (P<0.05). In addition, RSV upregulated Akt phosphorylation, Nrf2 phosphorylation, and expression levels of antioxidant genes HO-1, SOD-1, and CAT in a dose-dependent manner (P<0.05) under oxidative stress. Knockdown of Nrf2 by short-hairpin RNA (shRNA) abrogated RSV-mediated protection against H2O2-induced apoptosis, RSV-induced increase of TJ protein levels, and antioxidant gene expression (SOD-1, CAT, and GSH-Px) (P<0.05). Consistent with Nrf2 knockdown, the PI3K/Akt inhibitor LY294002 significantly suppressed RSV-induced Nrf2 phosphorylation and RSV-induced increase of TJ protein levels and antioxidant gene expression under H2O2 treatment (P<0.05). Collectively, these results demonstrate that RSV can directly protect IPEC-J2 cells against oxidative stress through the PI3K/Akt-mediated Nrf2 signaling pathway, suggesting that RSV may be an effective feed additive against intestinal damage in livestock production.

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