scholarly journals Evolutionary Convergence of Pathway-Specific Enzyme Expression Stoichiometry

Cell ◽  
2018 ◽  
Vol 173 (3) ◽  
pp. 749-761.e38 ◽  
Author(s):  
Jean-Benoît Lalanne ◽  
James C. Taggart ◽  
Monica S. Guo ◽  
Lydia Herzel ◽  
Ariel Schieler ◽  
...  
Keyword(s):  
Enzyme Expression ◽  
Specific Enzyme ◽  
Evolutionary Convergence

scholarly journals Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Reda Bellaouchi ◽  
Houssam Abouloifa ◽  
Yahya Rokni ◽  
Amina Hasnaoui ◽  
Nabil Ghabbour ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Culture Medium ◽  
Extracellular Enzymes ◽  
Enzyme Expression ◽  
Specific Enzyme ◽  
Enzymatic Production ◽  
High Production Rate ◽  
High Production ◽  
Incubation Temperatures ◽  
By Products

Abstract Background This work aims to study the optimal conditions of the fermentation culture medium used for the production of extracellular enzymes (amylase, cellulase, lipase, and protease) from previously isolated Aspergillus niger strains in date by-products. Results The five most powerful isolates selected based on the zone of degradation formed on Petri plates by the substrate were subjected to the quantitative evaluation of their enzymatic production. All five strains showed almost similar API-ZYM profiles, with minor variations observed at the level of some specific enzyme expression. The production of cellulase and amylase was depending on pH and incubation temperatures. ASP2 strain demonstrated the high production rate of amylase (at pH 5 and 30 °C) and cellulase (at pH 6 and 30 °C) for 96 h of incubation. Conclusion The A. niger showed the ability to produce several extracellular enzymes and can be used in the valorization of different agroindustrial residues.

Vascular Angiotensin-Converting Enzyme Expression Regulates Local Angiotensin II

Hypertension ◽  
1997 ◽  
Vol 29 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Dominik N. Müller ◽  
Jürgen Bohlender ◽  
Karl F. Hilgers ◽  
Duska Dragun ◽  
Olivier Costerousse ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Angiotensin Converting Enzyme ◽  
Converting Enzyme ◽  
Enzyme Expression

Differential effects of inhibitors of PTZ‐induced kindling on glutamate transporters and enzyme expression

2021 ◽  
Author(s):  
Numan Taspinar ◽  
Ahmet Hacimuftuoglu ◽  
Selcuk Butuner ◽  
Basak Togar ◽  
Gokhan Arslan ◽  
...  
Keyword(s):  
Glutamate Transporters ◽  
Enzyme Expression ◽  
Differential Effects

scholarly journals Disruption of the Complex between GAPDH and Hsp70 Sensitizes C6 Glioblastoma Cells to Hypoxic Stress

2021 ◽  
Vol 22 (4) ◽  
pp. 1520
Author(s):  
Marina A. Mikeladze ◽  
Elizaveta A. Dutysheva ◽  
Victor G. Kartsev ◽  
Boris A. Margulis ◽  
Irina V. Guzhova ◽  
...  
Keyword(s):  
Cell Death ◽  
Hypoxic Stress ◽  
C6 Cells ◽  
Tumor Resistance ◽  
Glioblastoma Cells ◽  
Enzyme Expression ◽  
Hsp70 Chaperone ◽  
Therapeutic Tools ◽  
Enzyme Molecules

Hypoxia, which commonly accompanies tumor growth, depending on its strength may cause the enhancement of tumorigenicity of cancer cells or their death. One of the proteins targeted by hypoxia is glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and we demonstrated here that hypoxia mimicked by treating C6 rat glioblastoma cells with cobalt chloride caused an up-regulation of the enzyme expression, while further elevation of hypoxic stress caused the enzyme aggregation concomitantly with cell death. Reduction or elevation of GAPDH performed with the aid of specific shRNAs resulted in the augmentation of the tumorigenicity of C6 cells or their sensitization to hypoxic stress. Another hypoxia-regulated protein, Hsp70 chaperone, was shown to prevent the aggregation of oxidized GAPDH and to reduce hypoxia-mediated cell death. In order to release the enzyme molecules from the chaperone, we employed its inhibitor, derivative of colchicine. The compound was found to substantially increase aggregation of GAPDH and to sensitize C6 cells to hypoxia both in vitro and in animals bearing tumors with distinct levels of the enzyme expression. In conclusion, blocking the chaperonic activity of Hsp70 and its interaction with GAPDH may become a promising strategy to overcome tumor resistance to multiple environmental stresses and enhance existing therapeutic tools.

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