scholarly journals SnapShot: Posttranscriptional Gene Silencing

Cell ◽  
2007 ◽  
Vol 130 (3) ◽  
pp. 570.e1-570.e2 ◽  
Author(s):  
Sigal Pressman ◽  
Yanxia Bei ◽  
Richard Carthew
Keyword(s):  
Gene Silencing ◽  
Posttranscriptional Gene Silencing

Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis

Science ◽  
2015 ◽  
Vol 348 (6230) ◽  
pp. 120-123 ◽  
Author(s):  
Xinyan Zhang ◽  
Ying Zhu ◽  
Xiaodan Liu ◽  
Xinyu Hong ◽  
Yang Xu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Plant Immunity ◽  
Rna Decay ◽  
Foreign Gene ◽  
Small Interfering Rnas ◽  
Developmental Defects ◽  
Posttranscriptional Gene Silencing ◽  
Endogenous Gene ◽  
Cytoplasmic Rna ◽  
Cognate Gene

Plant immunity against foreign gene invasion takes advantage of posttranscriptional gene silencing (PTGS). How plants elaborately avert inappropriate PTGS of endogenous coding genes remains unclear. We demonstrate in Arabidopsis that both 5′-3′ and 3′-5′ cytoplasmic RNA decay pathways act as repressors of transgene and endogenous PTGS. Disruption of bidirectional cytoplasmic RNA decay leads to pleiotropic developmental defects and drastic transcriptomic alterations, which are substantially rescued by PTGS mutants. Upon dysfunction of bidirectional RNA decay, a large number of 21- to 22-nucleotide endogenous small interfering RNAs are produced from coding transcripts, including multiple microRNA targets, which could interfere with their cognate gene expression and functions. This study highlights the risk of unwanted PTGS and identifies cytoplasmic RNA decay pathways as safeguards of plant transcriptome and development.

scholarly journals Effects of local mRNA structure on posttranscriptional gene silencing

2008 ◽  
Vol 105 (37) ◽  
pp. 13787-13792 ◽  
Author(s):  
S. I. Rudnick ◽  
J. Swaminathan ◽  
M. Sumaroka ◽  
S. Liebhaber ◽  
A. M. Gewirtz
Keyword(s):  
Gene Silencing ◽  
Posttranscriptional Gene Silencing ◽  
Mrna Structure

scholarly journals The Three Essential Motifs in P0 for Suppression of RNA Silencing Activity of Potato leafroll virus Are Required for Virus Systemic Infection

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 170 ◽  
Author(s):  
Mamun-Or Rashid ◽  
Xiao-Yan Zhang ◽  
Ying Wang ◽  
Da-Wei Li ◽  
Jia-Lin Yu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gene Silencing ◽  
Rna Silencing ◽  
Systemic Infection ◽  
Rna Degradation ◽  
Potato Leafroll Virus ◽  
Posttranscriptional Gene Silencing ◽  
Conserved Region ◽  
Leafroll Virus ◽  
The Impact

Higher plants exploit posttranscriptional gene silencing as a defense mechanism against virus infection by the RNA degradation system. Plant RNA viruses suppress posttranscriptional gene silencing using their encoded proteins. Three important motifs (F-box-like motif, G139/W140/G141-like motif, and C-terminal conserved region) in P0 of Potato leafroll virus (PLRV) were reported to be essential for suppression of RNA silencing activity. In this study, Agrobacterium-mediated transient experiments were carried out to screen the available amino acid substitutions in the F-box-like motif and G139/W140/G141-like motif that abolished the RNA silencing suppression activity of P0, without disturbing the P1 amino acid sequence. Subsequently, four P0 defective mutants derived from a full-length cDNA clone of PLRV (L76F and W87R substitutions in the F-box-like motif, G139RRR substitution in the G139/W140/G141-like motif, and F220R substitution in the C-terminal conserved region) were successfully generated by reverse PCR and used to investigate the impact of these substitutions on PLRV infectivity. The RT-PCR and western blot analysis revealed that these defective mutants affected virus accumulation in inoculated leaves and systemic movement in Nicotiana benthamiana as well as in its natural hosts, potato and black nightshade. These results further demonstrate that the RNA silencing suppressor of PLRV is required for PLRV accumulation and systemic infection.

scholarly journals The Nuclear Ribonucleoprotein SmD1 Interplays with Splicing, RNA Quality Control, and Posttranscriptional Gene Silencing in Arabidopsis

2016 ◽  
Vol 28 (2) ◽  
pp. 426-438 ◽  
Author(s):  
Emilie Elvira-Matelot ◽  
Florian Bardou ◽  
Federico Ariel ◽  
Vincent Jauvion ◽  
Nathalie Bouteiller ◽  
...  
Keyword(s):  
Quality Control ◽  
Gene Silencing ◽  
Posttranscriptional Gene Silencing ◽  
Rna Quality ◽  
Rna Quality Control ◽  
Nuclear Ribonucleoprotein

scholarly journals Dark Green Islands in Plant Virus Infection are the Result of Posttranscriptional Gene Silencing

2001 ◽  
Vol 14 (8) ◽  
pp. 939-946 ◽  
Author(s):  
Carolyn J. Moore ◽  
Paul W. Sutherland ◽  
Richard L. S. Forster ◽  
Richard C. Gardner ◽  
Robin M. MacDiarmid
Keyword(s):  
Gene Silencing ◽  
Plant Virus ◽  
Infected Tissue ◽  
Common Symptom ◽  
Yellow Virus ◽  
Posttranscriptional Gene Silencing ◽  
Heterologous Virus ◽  
Infected Cells ◽  
Dark Green ◽  
Lines Of Evidence

Dark green islands (DGIs) are a common symptom of plants systemically infected with a mosaic virus. DGIs are clusters of green leaf cells that are free of virus but surrounded by yellow, virus-infected tissue. We report here on two lines of evidence showing that DGIs are caused by posttranscriptional gene silencing (PTGS). First, transcripts of a transgene derived from the coat protein of Tamarillo mosaic potyvirus (TaMV) were reduced in DGIs relative to adjacent yellow tissues when the plants were infected with TaMV. Second, nontransgenic plants coinfected with TaMV and a heterologous virus vector carrying TaMV sequences showed reduced titers of the vector in DGIs compared with surrounding tissues. DGIs also were compared with recovered tissue at the top of transgenic plants because recovery has been shown previously to involve PTGS. Cytological analysis of the cells at the junction between recovered and infected tissue was undertaken. The interface between recovered and infected cells had very similar features to that surrounding DGIs. We conclude that DGIs and recovery are related phenomena, differing in their ability to amplify or transport the silencing signal.

scholarly journals Posttranscriptional gene silencing of gn1 in tobacco triggers accumulation of truncated gn1-derived RNA species

RNA ◽  
1999 ◽  
Vol 5 (10) ◽  
pp. 1364-1373 ◽  
Author(s):  
KATIA LITIÈRE ◽  
GERBEN J. VAN ELDIK ◽  
JOHN J.M.R. JACOBS ◽  
MARC VAN MONTAGU ◽  
MARC CORNELISSEN
Keyword(s):  
Gene Silencing ◽  
Posttranscriptional Gene Silencing

scholarly journals Computational Estimation and Experimental Verification of Off-Target Silencing during Posttranscriptional Gene Silencing in Plants

2006 ◽  
Vol 142 (2) ◽  
pp. 429-440 ◽  
Author(s):  
Ping Xu ◽  
Yuanji Zhang ◽  
Li Kang ◽  
Marilyn J. Roossinck ◽  
Kirankumar S. Mysore
Keyword(s):  
Gene Silencing ◽  
Experimental Verification ◽  
Posttranscriptional Gene Silencing ◽  
Computational Estimation
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