Effects of copper on a reconstructed freshwater rainbow trout gill epithelium: Paracellular and intracellular aspects

2020 ◽  
Vol 230 ◽  
pp. 108705
Author(s):  
Lygia S. Nogueira ◽  
Chun Chih Chen ◽  
Chris M. Wood ◽  
Scott P. Kelly
Keyword(s):  
Rainbow Trout ◽  
Gill Epithelium ◽  
Trout Gill

scholarly journals Characterization of antigen sampling cells in rainbow trout gill epithelium

2016 ◽  
Vol 53 ◽  
pp. 82-83 ◽  
Author(s):  
Goshi Kato ◽  
Haruya Miyazawa ◽  
Takuya Yamaguchi ◽  
Hidehiro Kondo ◽  
Motohiko Sano ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Gill Epithelium ◽  
Trout Gill

scholarly journals Cultured rainbow trout gill epithelium as an in vitro method for marine ecosystem toxicological studies

Heliyon ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. e08018
Author(s):  
Kafilat Adebola Bawa-Allah ◽  
Adebayo Otitoloju ◽  
Christer Hogstrand
Keyword(s):  
Rainbow Trout ◽  
Marine Ecosystem ◽  
Gill Epithelium ◽  
In Vitro Method ◽  
Toxicological Studies ◽  
Trout Gill

scholarly journals Differential Exoproteome and Biochemical Characterisation of Neoparamoeba perurans

2021 ◽  
Vol 9 (6) ◽  
pp. 1258
Author(s):  
Kerrie Ní Ní Dhufaigh ◽  
Natasha Botwright ◽  
Eugene Dillon ◽  
Ian O’Connor ◽  
Eugene MacCarthy ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Virulence Factors ◽  
Cytotoxic Effect ◽  
Differentially Expressed Proteins ◽  
Biochemical Characterisation ◽  
Key Players ◽  
Prolonged Culture ◽  
Global Threat ◽  
Iron Receptor ◽  
Trout Gill

Infection with the protozoan ectoparasite Neoparamoeba perurans, the causative agent of AGD, remains a global threat to salmonid farming. This study aimed to analyse the exoproteome of both an attenuated and virulent N. perurans isolate using proteomics and cytotoxicity testing. A disproportionate presence of proteins from the co-cultured microbiota of N. perurans was revealed on searching an amalgamated database of bacterial, N. perurans and Amoebozoa proteins. LC‑MS/MS identified 33 differentially expressed proteins, the majority of which were upregulated in the attenuated exoproteome. Proteins of putative interest found in both exoproteomes were maltoporin, ferrichrome-iron receptor, and putative ferric enterobactin receptor. Protease activity remained significantly elevated in the attenuated exoproteome compared with the virulent exoproteome. Similarly, the attenuated exoproteome had a significantly higher cytotoxic effect on rainbow trout gill cell line (RTgill W1) cells compared with the virulent exoproteome. The presence of a phosphatase and serine protease in the virulent exoproteome may facilitate AGD infection but do not appear to be key players in causing cytotoxicity. Altogether, this study reveals prolonged culture of N. perurans affects the exoproteome composition in favour of nutritional acquisition, and that the current culturing protocol for virulent N. perurans does not facilitate the secretion of virulence factors.

Responses of trout gill ion transport systems to acute acidosis

1976 ◽  
Vol 64 (2) ◽  
pp. 511-515
Author(s):  
T. H. Kerstetter ◽  
R. Mize
Keyword(s):  
Lactic Acid ◽  
Rainbow Trout ◽  
Ion Transport ◽  
Ion Uptake ◽  
Transport Systems ◽  
Ph Changes ◽  
Blood Ph ◽  
Slow Infusion ◽  
Trout Gill

The response of rainbow trout Na+ and Cl- uptake systems to acute acidosis was tested by slow infusion of lactic acid into anaesthetized animals. Depression of blood pH by 0–4 pH unit had no effect on influx rates for either ion, and we conclude that gill ion uptake systems do not respond rapidly to blood pH changes.

scholarly journals Tricellular tight junction-associated angulins in the gill epithelium of rainbow trout

2018 ◽  
Vol 315 (2) ◽  
pp. R312-R322 ◽  
Author(s):  
Dennis Kolosov ◽  
Scott P. Kelly
Keyword(s):  
Tight Junction ◽  
Transcript Abundance ◽  
Barrier Properties ◽  
Mrna Abundance ◽  
Gill Epithelium ◽  
Molecular Physiology ◽  
Permeability Characteristics ◽  
Gill Cells ◽  
Associated Proteins ◽  
Trout Gill

Molecular physiology of the tricellular tight junction (tTJ)-associated proteins lipolysis-stimulated lipoprotein receptor ( lsr, = angulin-1) and an immunoglobulin-like domain-containing receptor ( ildr2, ≈angulin-3) was examined in model trout gill epithelia. Transcripts encoding lsr and ildr2 are broadly expressed in trout organs. A reduction in lsr and ildr2 mRNA abundance was observed during and after confluence in flask-cultured gill cells. In contrast, as high-resistance and low-permeability characteristics developed in a model gill epithelium cultured on permeable polyethylene terephthalate membrane inserts, lsr and ildr2 transcript abundance increased. However, as epithelia entered the developmental plateau phase, lsr abundance returned to initial values, while ildr2 transcript abundance remained elevated. When mitochondrion-rich cells were introduced to model preparations, lsr mRNA abundance was unaltered and ildr2 mRNA abundance significantly increased. Transcript abundance of ildr2 was not altered in association with corticosteroid-induced tightening of the gill epithelium, while lsr mRNA abundance decreased. Transcriptional knockdown of the tTJ protein tricelluin (Tric) reduced Tric abundance, increased gill epithelium permeability, and increased lsr without significantly altering ildr2 transcript abundance. Data suggest that angulins contribute to fish gill epithelium barrier properties but that Lsr and Ildr2 seem likely to play different roles. This is because ildr2 typically exhibited increased abundance in association with decreased model permeability, while lsr abundance changed in a manner that suggested a role in Tric recruitment to the tTJ.

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