Biotransformation of PCBs in Arctic seabirds: Characterization of phase I and II pathways at transcriptional, translational and activity levels

2010 ◽  
Vol 152 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Lisa B. Helgason ◽  
Augustine Arukwe ◽  
Geir W. Gabrielsen ◽  
Mikael Harju ◽  
Marit N. Hegseth ◽  
...  
Keyword(s):  
Phase I ◽  
Activity Levels ◽  
Arctic Seabirds ◽  
Phase I And Ii

Isolation and partial characterization of a lipopolysaccharide from phase II Coxiella burnetii

1980 ◽  
Vol 26 (7) ◽  
pp. 819-826 ◽  
Author(s):  
Oswald G. Baca ◽  
Irene L. Martinez ◽  
Adam S. Aragón ◽  
David Klassen
Keyword(s):  
Phase I ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Complement Fixation ◽  
Fatty Acid Profiles ◽  
Versus Phase ◽  
History Of ◽  
Phenol Water ◽  
Phase I And Ii

A lipopolysaccharide (LPS) was isolated by hot phenol–water extraction from phase II Coxiella burnetii. The LPS was isolated from organisms that had a history of 95 serial yolk sac passages and others that were cloned. The chemical composition of the LPS was partially determined and compared with that of LPS previously isolated from phase I organisms. Most of the sugars present in phase I LPS were detected in phase II LPS; however, there were some quantitative and qualitative differences. The fatty acid profiles of phase I and II LPS were identical. The amount of LPS extracted from phase II organisms was about [Formula: see text] the amount extracted from phase I C. burnetii. Limulus lysate gelation was observed with subnanogram amounts of both phase I and II LPS. In complement fixation tests, both LPSs reacted only with antisera containing antibodies versus phase I antigen. Ouchterlony immunodiffusion tests demonstrated immunological identity of the two LPSs.

scholarly journals Combining HPLC-DAD-QTOF-MS and HPLC-SPE-NMR to Monitor In Vitro Vitetrifolin D Phase I and II Metabolism

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 529
Author(s):  
Sonja Sturm ◽  
Christina Högner ◽  
Christoph Seger ◽  
Hermann Stuppner
Keyword(s):  
Phase I ◽  
Culture Broth ◽  
In Vitro Metabolism ◽  
Successful Performance ◽  
Cell Fractions ◽  
Pharmacologically Active ◽  
Phase Ii Metabolites ◽  
Phase I And Ii

By combining HPLC-DAD-QTOF-MS and HPLC-SPE-NMR, the in vitro metabolism of vitetrifolin D, a pharmacologically active key molecule from Vitex agnus-castus in liver cell fractions, was investigated. Twenty-seven phase I and phase II metabolites were tentatively identified from the culture broth by HPLC-DAD-QTOF-MS. The subsequent HPLC-SPE-NMR analysis allowed for the unequivocal structural characterization of nine phase I metabolites. Since the preparative isolation of the metabolites was avoided, the substance input was much lower than in conventional strategies. The study did prove that the use of hyphenated instrumental analysis methodologies allows for the successful performance of in vitro metabolism studies, even if the availability of substances is very limited.

Phase I and II Archaeological Investigations at Shaw Air Force Base and the Poinsett Electronic Combat Range, Sumter County, South Carolina

2005 ◽  
Author(s):  
Alvin J. Banguilan ◽  
John S. Cable ◽  
Charles E. Cantley ◽  
Leslie Raymer ◽  
Victoria Dabir-Banguilan
Keyword(s):  
South Carolina ◽  
Phase I ◽  
Air Force ◽  
Air Force Base ◽  
Phase I And Ii

scholarly journals Osteosarcoma Pathogenesis Leads the Way to New Target Treatments

2021 ◽  
Vol 22 (2) ◽  
pp. 813
Author(s):  
Isabel Fernandes ◽  
Cecília Melo-Alvim ◽  
Raquel Lopes-Brás ◽  
Miguel Esperança-Martins ◽  
Luís Costa
Keyword(s):  
Clinical Trials ◽  
Phase I ◽  
Poor Prognosis ◽  
Therapeutic Targets ◽  
Rare Condition ◽  
Basic Research ◽  
Metastatic Setting ◽  
Phase I And Ii ◽  
The Way

Osteosarcoma (OS) is a rare condition with very poor prognosis in a metastatic setting. Basic research has enabled a better understanding of OS pathogenesis and the discovery of new potential therapeutic targets. Phase I and II clinical trials are already ongoing, with some promising results for these patients. This article reviews OS pathogenesis and new potential therapeutic targets.

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