Subcellular fractionation of Cu exposed oysters, Crassostrea virginica, and Cu accumulation from a biologically incorporated Cu rich oyster diet in Fundulus heteroclitus in fresh and sea water

2009 ◽  
Vol 149 (4) ◽  
pp. 531-537 ◽  
Author(s):  
Jonathan Blanchard ◽  
Kevin Brix ◽  
Martin Grosell
Keyword(s):  
Crassostrea Virginica ◽  
Fundulus Heteroclitus ◽  
Sea Water ◽  
Subcellular Fractionation ◽  
Cu Accumulation

Cadmium Uptake by Marine Organisms

1972 ◽  
Vol 29 (9) ◽  
pp. 1367-1369 ◽  
Author(s):  
R. Eisler ◽  
G. E. Zaroogian ◽  
R. J. Hennekey
Keyword(s):  
Crassostrea Virginica ◽  
Fundulus Heteroclitus ◽  
Sea Water ◽  
Homarus Americanus ◽  
Adductor Muscle ◽  
Marine Organisms ◽  
Cadmium Uptake ◽  
Wet Weight

Adults of mummichog, Fundulus heteroclitus, scallop Aquipecten irradians, oyster Crassostrea virginica, and subadult lobsters Homarus americanus were immersed for 21 days in flowing sea water containing 10 μg/liter of cadmium as[Formula: see text]. Cadmium residues in whole animals and selected tissues were consistently higher in exposed organisms than controls; edible portions of treated lobster (muscle), scallop (adductor muscle), and oyster (whole animal) contained more cadmium per unit wet weight than controls by 25%, 19%, and 352%, respectively.

The accumulation and excretion of radioactive caesium by the plaice (Pleuronectes platessa) and the thornback ray (Raia clavata)

1971 ◽  
Vol 51 (2) ◽  
pp. 411-422 ◽  
Author(s):  
D. F. Jefferies ◽  
C. J. Hewett
Keyword(s):  
Nuclear Power ◽  
Fundulus Heteroclitus ◽  
Sea Water ◽  
Marine Species ◽  
Whole Body ◽  
Turnover Rates ◽  
Power Stations ◽  
The United Kingdom ◽  
Thornback Ray ◽  
Processing Plants

INTRODUCTIONThe caesium radionuclides, caesium-137 and caesium-134, are important constituents of aqueous radioactive effluents discharged to the environment of the United Kingdom from fuel re-processing plants and nuclear power stations (Howells, 1966; Mitchell, Harvey & Smith, 1968; Harvey, Baker & Mitchell, 1969). Their accumulation by marine fishes has been the subject of several previous studies, beginning with that of Chipman (1959) who noted that caesium-137 was accumulated in the flesh of the killifish (Fundulus heteroclitus) and that accumulation continued even after a period of 72 days. Similarly, Hiyama & Shimizu (1964) showed that the muscle of the common goby (Acanthobus flaviamus) continued to accumulate caesium-134 from sea water after periods of 30 days. Baptist & Price (1962) have examined the whole body uptake of caesium, by absorption from sea water, in two marine species, the flounder (Paralichthys dentatus) and the Atlantic croaker (Micropogon undulatus), and they also investigated the accumulation, tissue distribution and excretion of caesium-137 in the croaker, the little tuna (Euthynnus alleteratus) and the bluefish (Pomatomus saltatoux), following oral administration of single doses. These authors concluded that the heart, liver and spleen of the croaker absorbed caesium-137 from sea water at a faster rate than the muscle, and that orally administered caesium-137 was rapidly absorbed from the digestive tract. Tissue distributions were similar in the croaker, bluefish and tuna. The retention of caesium-137 in croaker tissue was described as a multiple rate process. In later experiments Hiyama & Shimizu (1969) compared turnover rates in various organs and tissues following uptake from sea water and from single injection experiments, and obtained good agreement between the values from the two methods.

Thermal preferences of mummichog, Fundulus heteroclitus L., and banded killifish, F. diaphanus (LeSueur), (Cyprinodontidae) in relation to thermal acclimation and salinity

1977 ◽  
Vol 55 (7) ◽  
pp. 1190-1194 ◽  
Author(s):  
E. T. Garside ◽  
G. C. Morrison
Keyword(s):  
Fresh Water ◽  
Fundulus Heteroclitus ◽  
Sea Water ◽  
Thermal Acclimation ◽  
Thermal Gradients ◽  
Frequency Distributions ◽  
Preferred Temperatures ◽  
Typical Habitat ◽  
Thermal Preferences ◽  
Banded Killifish

Frequency distributions corresponding to various levels of thermal acclimation from 5 to 35 °C were recorded for samples of marine mummichog, Fundulus heteroclitus L., and samples of freshwater banded killifish, F. diaphanus (LeSueur), in thermal gradients formed in columns of fresh water (< 0.5‰ salinity) or sea water (32‰ salinity). Mean preferred temperatures comprised roughly parallel but irregularly inflected trends for the two series of tests, within each species. In mummichog, preferred temperatures for corresponding thermal acclimations ranged from 3 to 6 °C higher in tests conducted in sea water. In banded killifish, preferred temperatures for corresponding thermal acclimations ranged from 5 to 8 °C higher in fresh water. Thus, each species regularly preferred higher temperatures in salinity which approximated that of the typical habitat. The inference to be taken is that the unusual salinity for each species places an extraordinary osmoregulative load which influences the reactions of the fish to the series of thermal or other correlated stimuli in the gradient.

Removal of Toxoplasma gondii Oocysts from Sea Water by Eastern Oysters (Crassostrea virginica)

2001 ◽  
Vol 48 ◽  
pp. 197s-198s ◽  
Author(s):  
DAVID S. LINDSAY ◽  
KALMIA K. PHELPS ◽  
STEPHEN A. SMITH ◽  
GEORGE FLICK ◽  
SUSAN S. SUMNER ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Crassostrea Virginica ◽  
Sea Water ◽  
Eastern Oysters

scholarly journals Time course of salinity adaptation in a strongly euryhaline estuarine teleost, fundulus heteroclitus: a multivariable approach

1999 ◽  
Vol 202 (11) ◽  
pp. 1535-1544 ◽  
Author(s):  
W.S. Marshall ◽  
T.R. Emberley ◽  
T.D. Singer ◽  
S.E. Bryson ◽  
S.D. Mccormick
Keyword(s):  
Fresh Water ◽  
Plasma Cortisol ◽  
Time Course ◽  
Fundulus Heteroclitus ◽  
Citrate Synthase ◽  
Sea Water ◽  
Short Circuit ◽  
Secretion Rate ◽  
Ion Secretion ◽  
Cortisol Levels

Freshwater-adapted killifish (Fundulus heteroclitus) were transferred directly from soft fresh water to full-strength sea water for periods of 1 h, 3 h, 8 h and 1, 2, 7, 14 and 30 days. Controls were transferred to fresh water for 24 h. Measured variables included: blood [Na+], osmolality, glucose and cortisol levels, basal and stimulated rates of ion transport and permeability of in vitro opercular epithelium, gill Na+/K+-ATPase and citrate synthase activity and chloride cell ultrastructure. These data were compared with previously published killifish cystic fibrosis transmembrane conductance regulator (kfCFTR) expression in the gills measured over a similar time course. Plasma cortisol levels peaked at 1 h, coincident with a rise in plasma [Na+]. At 8 h after transfer to sea water, a time at which previous work has shown kfCFTR expression to be elevated, blood osmolality and [Na+] were high, and cortisol levels and opercular membrane short-circuit current (Isc; a measure of Cl- secretion rate) were low. The 24 h group, which showed the highest level of kfCFTR expression, had the highest plasma [Na+] and osmolality, elevated plasma cortisol levels, significantly lower opercular membrane resistance, an increased opercular membrane ion secretion rate and collapsed tubule inclusions in mitochondria-rich cells, but no change in gill Na+/K+-ATPase and citrate synthase activity or plasma glucose levels. Apparently, killifish have a rapid (&lt;1 h) cortisol response to salinity coupled to subsequent (8–48 h) expression of kfCFTR anion channel proteins in existing mitochondria-rich cells that convert transport from ion uptake to ion secretion.

A novel 14-3-3 gene is osmoregulated in gill epithelium of the euryhaline teleost Fundulus heteroclitus

2001 ◽  
Vol 204 (17) ◽  
pp. 2975-2985 ◽  
Author(s):  
Dietmar Kültz ◽  
Devulapalli Chakravarty ◽  
Tadepalli Adilakshmi
Keyword(s):  
Signal Transduction ◽  
Amino Acid ◽  
Fundulus Heteroclitus ◽  
Sea Water ◽  
Amino Acid Residues ◽  
Gill Epithelium ◽  
Protein Levels ◽  
Euryhaline Teleost ◽  
Binding Groove ◽  
High Level

SUMMARY We have cloned and analyzed the full-length coding sequence and 3′ untranslated region (UTR) of a unique 14-3-3 gene of the euryhaline teleost Fundulus heteroclitus, which we named 14-3-3.a. Phylogenetic analysis of the deduced amino acid sequence revealed that the 14-3-3.a gene product is most similar to vertebrate 14-3-3ζ and β, yet it displays considerable divergence to known classes of vertebrate 14-3-3 isoforms. The N and C termini of 14-3-3.a are the most unique regions, whereas the amino acid residues forming the amphipathic ligand-binding groove are highly conserved. F. heteroclitus 14-3-3.a mRNA expression is high in gill epithelium, moderate in intestine and brain, and low in gonads, white muscle and heart. Because 14-3-3 proteins are important molecular scaffolds and cofactors for phosphoproteins and signaling complexes, the high level of 14-3-3.a expression in gill epithelium of the euryhaline teleost F. heteroclitus suggests that it is crucial for signal transduction in gill epithelial cells. We provide evidence that 14-3-3.a is involved in osmosensory signal transduction by showing that its mRNA and protein levels in gill epithelium, but not in any other tissue analyzed, increase two- to fourfold within 24h of salinity transfer of fish from sea water to fresh water. These data are clear evidence for an important role of 14-3-3.a in the remodeling of gill epithelium during transition of euryhaline fish between plasma-hyperosmotic and plasma-hyposmotic environments.

Uptake and Elimination of Bacteria in Shellfish*

1980 ◽  
Vol 43 (2) ◽  
pp. 124-126 ◽  
Author(s):  
FRANK O. PERKINS ◽  
DEXTER S. HAVEN ◽  
REINALDO MORALES-ALAMO ◽  
MARTHA W. RHODES
Keyword(s):  
Crassostrea Virginica ◽  
Environmental Conditions ◽  
Fecal Coliform ◽  
Sea Water ◽  
Fecal Coliforms ◽  
Most Probable Number ◽  
Probable Number ◽  
General Review ◽  
Wide Range ◽  
Bacterial Accumulation

A general review of knowledge concerning bacterial accumulation and depletion by commercially significant bivalve molluses is presented. Naturally contaminated shellfish can eliminate fecal coliforms (FC) in 48 h to levels below most market standards over a wide range of environmental conditions when sea water flowing to the molluses is treated so that fecal coliform levels are indeterminate or marginally determinate as assayed by standard methodology. Most probable number (MPN) enumerations of shellfish depurated for 48 h by the authors yielded a median value of &lt; 18 FC/100 g of oyster (Crassostrea Virginica) meats with &lt; 10% of the samples exceeding 78 FC/100 g.

Quantitative and Qualitative Studies of Bacteria Isolated from Sea Water Used in the Laboratory Culture of the American Oyster, Crassostrea virginica

1975 ◽  
Vol 32 (6) ◽  
pp. 739-745 ◽  
Author(s):  
R. A. Murchelano ◽  
C. Brown ◽  
J. Bishop
Keyword(s):  
Ultraviolet Light ◽  
Crassostrea Virginica ◽  
Storage Tank ◽  
Sea Water ◽  
Sampling Period ◽  
Laboratory Culture ◽  
Qualitative Studies ◽  
American Oyster ◽  
The Mean ◽  
Number Of Bacteria

Quantitative and qualitative bacteriological studies were conducted on sea water utilized in laboratory culture of American oyster larvae (Crassostrea virginica).The generic diversity of bacteria isolated from a laboratory seawater storage tank did not change during a 15-day sampling period, although bacterial numbers increased substantially. The mean number of bacteria in ultraviolet light-irradiated sea water was less than that in nonirradiated sea water, although individual samples varied considerably. Over a 10-day sampling period during which oyster larval cultures were sampled repetitively, bacteria were more numerous at 24 h and 48 h than at 0 h.Pseudomonas was the dominant genus present in stored sea water, irradiated sea water, and sea water from oyster larval cultures.

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