Molecular cloning of hepatic glucose-6-phosphatase catalytic subunit from gilthead sea bream (Sparus aurata): response of its mRNA levels and glucokinase expression to refeeding and diet composition

2004 ◽  
Vol 138 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Isidoro Metón ◽  
Anna Caseras ◽  
Felipe Fernández ◽  
Isabel V. Baanante
Keyword(s):  
Molecular Cloning ◽  
Diet Composition ◽  
Sparus Aurata ◽  
Catalytic Subunit ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Mrna Levels ◽  
Hepatic Glucose

scholarly journals Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)

2002 ◽  
Vol 88 (6) ◽  
pp. 607-614 ◽  
Author(s):  
A. Caseras ◽  
I. Metón ◽  
C. Vives ◽  
M. Egea ◽  
F. Fernández ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Metabolic Adaptation ◽  
Mrna Levels ◽  
Nutritional Regulation ◽  
Carnivorous Fish ◽  
Hepatic Expression ◽  
Glucose Levels

To examine the role of glucose-6-phosphatase (G6Pase) in glucose homeostasis in the diabetes-like experimental model of carnivorous fish, we analysed postprandial variations and the effect of starvation, ration size and diet composition on the regulation of G6Pase expression at the enzyme activity and mRNA level in the liver of gilthead sea bream (Sparus aurata). G6Pase expression increased in long-term starved or energy-restricted fish. In contrast to data reported for other fish species, short-term regulation of G6Pase expression was found in regularly fedS. aurata. G6Pase mRNA levels were lowest between 4 and 15 h after food intake, whereas minimal enzyme activity was observed 10–15 h postprandially. Alterations of plasma glucose levels affect G6Pase in mammals. However, the carbohydrate content of the diet did not affect hepatic expression of G6Pase inS. aurata, suggesting that a different molecular mechanism is involved in the control of G6Pase expression in fish. Although G6Pase was unaffected, high-carbohydrate low-protein diets increased glucokinase (GK) expression and thus allowed a metabolic adaptation favouring glycolysis over gluconeogenesis. Interestingly, only the nutritional conditions that promoted variations in the blood glucose levels resulted in changes in the hepatic expression of G6Pase. These findings indicate a concerted regulation of G6Pase and GK expression and suggest that the direction and rate of the glucose–glucose-6-phosphate substrate cycle flux is finely regulated in the liver ofS. aurata, challenging the role attributed to deficient regulation of G6Pase or GK expression in the low ability of carnivorous fish to metabolize glucose.

Molecular cloning and characterization of follistatin in the gilthead sea bream, Sparus aurata

2008 ◽  
Vol 36 (3) ◽  
pp. 501-511 ◽  
Author(s):  
Bruria Funkenstein ◽  
Yanai Rebhan ◽  
Tal Skopal
Keyword(s):  
Molecular Cloning ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream

scholarly journals Characterisation and Expression of Calpain Family Members in Relation to Nutritional Status, Diet Composition and Flesh Texture in Gilthead Sea Bream (Sparus aurata)

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e75349 ◽  
Author(s):  
Cristina Salmerón ◽  
Daniel García de la serrana ◽  
Vanesa Jiménez-Amilburu ◽  
Ramón Fontanillas ◽  
Isabel Navarro ◽  
...  
Keyword(s):  
Nutritional Status ◽  
Diet Composition ◽  
Sparus Aurata ◽  
Family Members ◽  
Gilthead Sea Bream ◽  
Sea Bream

Diet Composition of Gilthead Sea Bream Sparus Aurata from Bardawil Lagoon, North Sinai, Egypt

2020 ◽  
Vol 24 (3) ◽  
pp. 157-168
Author(s):  
Samah A. Mokbel ◽  
Hassanen G. D.I. ◽  
Nesreen K. Ibrahim ◽  
Salem M.
Keyword(s):  
Diet Composition ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
North Sinai

scholarly journals Diet and Exercise Modulate GH-IGFs Axis, Proteolytic Markers and Myogenic Regulatory Factors in Juveniles of Gilthead Sea Bream (Sparus aurata)

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2182
Author(s):  
Miquel Perelló-Amorós ◽  
Isabel García-Pérez ◽  
Albert Sánchez-Moya ◽  
Arnau Innamorati ◽  
Emilio J. Vélez ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
High Energy ◽  
Culture Conditions ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Mrna Levels ◽  
Myogenic Regulatory Factors ◽  
Regulatory Factors ◽  
Gene And Protein Expression ◽  
Plasma Gh

The physiological and endocrine benefits of sustained exercise in fish were largely demonstrated, and this work examines how the swimming activity can modify the effects of two diets (high-protein, HP: 54% proteins, 15% lipids; high-energy, HE: 50% proteins, 20% lipids) on different growth performance markers in gilthead sea bream juveniles. After 6 weeks of experimentation, fish under voluntary swimming and fed with HP showed significantly higher circulating growth hormone (GH) levels and plasma GH/insulin-like growth-1 (IGF-1) ratio than fish fed with HE, but under exercise, differences disappeared. The transcriptional profile of the GH-IGFs axis molecules and myogenic regulatory factors in liver and muscle was barely affected by diet and swimming conditions. Under voluntary swimming, fish fed with HE showed significantly increased mRNA levels of capn1, capn2, capn3, capns1a, n3, and ub, decreased gene and protein expression of Ctsl and Mafbx and lower muscle texture than fish fed with HP. When fish were exposed to sustained exercise, diet-induced differences in proteases’ expression and muscle texture almost disappeared. Overall, these results suggest that exercise might be a useful tool to minimize nutrient imbalances and that proteolytic genes could be good markers of the culture conditions and dietary treatments in fish.

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