A practicable mesostructured MFI zeolitic catalyst for large molecule reactions

2016 ◽  
Vol 78 ◽  
pp. 68-70 ◽  
Author(s):  
Haixia Miao ◽  
Weimin Zhang ◽  
Shufang Hu ◽  
Jinghong Ma ◽  
Ruifeng Li
Keyword(s):  
Large Molecule ◽  
Zeolitic Catalyst

scholarly journals P2X7 Receptors and TMEM16 Channels Are Functionally Coupled with Implications for Macropore Formation and Current Facilitation

2021 ◽  
Vol 22 (12) ◽  
pp. 6542
Author(s):  
Kate Dunning ◽  
Adeline Martz ◽  
Francisco Andrés Peralta ◽  
Federico Cevoli ◽  
Eric Boué-Grabot ◽  
...  
Keyword(s):  
Single Channel ◽  
Cell Membrane Permeability ◽  
Large Molecule ◽  
Cholesterol Depletion ◽  
Open Probability ◽  
P2x7 Receptors ◽  
Biochemical Assays ◽  
Functional Complex ◽  
Macropore Formation ◽  
Current Facilitation

P2X7 receptors (P2X7) are cationic channels involved in many diseases. Following their activation by extracellular ATP, distinct signaling pathways are triggered, which lead to various physiological responses such as the secretion of pro-inflammatory cytokines or the modulation of cell death. P2X7 also exhibit unique behaviors, such as “macropore” formation, which corresponds to enhanced large molecule cell membrane permeability and current facilitation, which is caused by prolonged activation. These two phenomena have often been confounded but, thus far, no clear mechanisms have been resolved. Here, by combining different approaches including whole-cell and single-channel recordings, pharmacological and biochemical assays, CRISPR/Cas9 technology and cell imaging, we provide evidence that current facilitation and macropore formation involve functional complexes comprised of P2X7 and TMEM16, a family of Ca2+-activated ion channel/scramblases. We found that current facilitation results in an increase of functional complex-embedded P2X7 open probability, a result that is recapitulated by plasma membrane cholesterol depletion. We further show that macropore formation entails two distinct large molecule permeation components, one of which requires functional complexes featuring TMEM16F subtype, the other likely being direct permeation through the P2X7 pore itself. Such functional complexes can be considered to represent a regulatory hub that may orchestrate distinct P2X7 functionalities.

Large molecule fraction of plasma separated with polyethylene glycol in treatment of chronic relapsing thrombotic thrombocytopenic purpura

1995 ◽  
Vol 49 (2) ◽  
pp. 149-152 ◽  
Author(s):  
Masahiro Fujita ◽  
Kazuhiko Ito ◽  
Tetsuji Koyama ◽  
Kazuko Shino ◽  
Mihoko Norioka ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Thrombotic Thrombocytopenic Purpura ◽  
Large Molecule ◽  
Thrombocytopenic Purpura
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