Gastric cancer cell detection in peritoneal lavage: RT-PCR for carcinoembryonic antigen transcripts versus the combined cytology with peritoneal carcinoembryonic antigen levels

2005 ◽  
Vol 223 (1) ◽  
pp. 129-135 ◽  
Author(s):  
Jaw-Yuan Wang ◽  
Shiu-Ru Lin ◽  
Chien-Yu Lu ◽  
Chung-Chi Chen ◽  
Deng-Chyang Wu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Carcinoembryonic Antigen ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Peritoneal Lavage ◽  
Cell Detection ◽  
Rt Pcr ◽  
Cancer Cell Detection

Gastric Cancer Cell Detection in Peritoneal Washing: Cytology Versus RT-PCR for CEA Transcripts

2003 ◽  
Vol 12 (2) ◽  
pp. 88-95 ◽  
Author(s):  
Elaine M. C. To ◽  
Wing-Yee Chan ◽  
Chit Chow ◽  
Enders Kwok-Wai Ng ◽  
Sheung-Chi Sydney Chung
Keyword(s):  
Gastric Cancer ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Cell Detection ◽  
Rt Pcr ◽  
Peritoneal Washing ◽  
Peritoneal Washing Cytology ◽  
Cancer Cell Detection

scholarly journals Circ_0000190 favors gastric cancer patients potentially via inhibiting miR-1252/PAK3 pathway

2020 ◽  
Author(s):  
Gui Jun Wang ◽  
Tian Yu Yu ◽  
Yan Rong Li ◽  
Yang Jun Liu ◽  
Bei-Bei Deng
Keyword(s):  
Gastric Cancer ◽  
Cell Viability ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Luciferase Reporter ◽  
Circular Rnas ◽  
Cancer Tissue ◽  
Human Gastric Cancer ◽  
Rt Pcr ◽  
And Migration

Abstract Background Gastric cancer accounts for 8.3% of all cancer death and is demonstrated associated with aberrant circular RNAs (circRNAs) expressions.Circ_0000190 has been noted with prognostic role in gastric cancer. We aim to investigate the role and the underlying mechanism of circ_0000190 in gastric cancer.Methods Circ_0000190 expressions were examined in gastric cancer and adjacent normal tissues by RT-PCR. With gastric cancer cell lines, circ_0000190 expression was detected by FISH and RT-PCR. After forced expression, the role of the circRNA in gastric cancer cell viability, apoptosis, proliferation, cell cycle and migration was observed. The potential effector of circ_0000190 was predicted by computational screen and validated by luciferase reporter assay. The association of the effectors with circ_0000190’s effects above mentioned were examined. Mice model of human gastric cancer was established to observe the underlying mechanisms of circ_0000190.Results Circ_0000190 was down-regulated in gastric cancer tissue and cells, with a major location in cytoplasm. After transfection, circ_0000190 inhibited gastric cancer cell viability, proliferation and migration, and induced apoptosis and cycle, which was correlated with increased capase-3 and p27 expression, and decreased cyclinD expression. The circRNA was validated as a sponge of miR-1252, which directly targeted PAK3. The effects by circ_0000190 on the cellular processes above mentioned were blocked by miR-1252 mimics, and this was rescued after further overexpression of PAK3.Conclusions Our results revealed that circ_0000190 protected against gastric cancer, and this was via directly targeting miR-767-5p/PAK3 axis. Therefore, employing circ_0000190 might be a promising therapeutic strategy for treatment of gastric cancer.

scholarly journals Huaier Granule Combined with Tegafur Gimeracil Oteracil Potassium Promotes Stage IIb Gastric Cancer Prognosis and Induces Gastric Cancer Cell Apoptosis by Regulating Livin

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Jing Qi ◽  
Fu-juan Xie ◽  
Sheng Liu ◽  
Cheng-yu Yao ◽  
Wei-hang Liu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Survival Rate ◽  
Western Blot ◽  
Cancer Cell ◽  
Cell Apoptosis ◽  
Gastric Cancer Cell ◽  
Cancer Prognosis ◽  
Rt Pcr ◽  
Cancer Cell Apoptosis

Gastric cancer is one of the most common malignancies worldwide, with high morbidity and poor survival rate. Its prognosis remains unsatisfactory, with a 5-year survival rate of <30%. Studies have indicated that Huaier granules have good antitumor efficacy and safety in several solid malignant tumors. Recent studies have also found that Huaier polysaccharides can promote apoptosis in numerous tumor cells, although only few studies have focused on the effects of Huaier granules on gastric cancers and the mechanisms underlying their antitumor role. We retrospectively evaluated stage IIb gastric cancer patients at Xiangya Hospital, Central South University, through our outpatient system from January 2013 to December 2015. Fifty-four patients were in the Huaier+Tegafur Gimeracil Oteracil Potassium (TGOP) group and 72 in the TGOP group. Further, we conducted CCK8, colony formation, Annexin V-FITC/PI, Western blot, RT-PCR, and plasmid transfection assays to analyze the mechanism by which Huaier polysaccharides play an antitumor role. We confirmed that Huaier granules combined with Tegafur Gimeracil Oteracil Potassium could promote patient prognosis, with a better disease-free survival rate (51.32±2.23 vs. 44.19±2.26, p=0.034) and overall survival rate (56.81±1.32 vs. 51.32±1.69, p=0.020). Moreover, through cell proliferation assays, Western blot, RT-PCR, and detection of Livin expression at the mRNA and protein levels, we found that Huaier polysaccharides could promote gastric cancer cell apoptosis and inhibit gastric cancer cell proliferation in a time- and dose-dependent manner. Finally, we demonstrated that Huaier polysaccharides promote gastric cancer cell apoptosis through the regulation of Livin expression. Overexpression of Livin reversed the gastric cell apoptosis induced by Huaier polysaccharides. Huaier granules combined with Tegafur Gimeracil Oteracil Potassium ameliorated stage IIb gastric cancer prognosis and induced gastric cancer cell apoptosis by regulating Livin.

scholarly journals Circ_0000190 favors gastric cancer patients potentially via inhibiting miR-1252/PAK3 pathway

2020 ◽  
Author(s):  
Gui Jun Wang ◽  
Tian Yu Yu ◽  
Yan Rong Li ◽  
Yang Jun Liu ◽  
Bei-Bei Deng
Keyword(s):  
Gastric Cancer ◽  
Cell Cycle ◽  
Cell Viability ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Luciferase Reporter ◽  
Circular Rnas ◽  
Human Gastric Cancer ◽  
Rt Pcr ◽  
And Migration

Abstract Background: Gastric cancer is a serious malignant tumor associated with aberrant circular RNAs (circRNAs) expression. In this study, we aim to investigate the role and the underlying mechanism of circ_0000190, a circRNA in gastric cancer.Methods: Circ_0000190 expression in vivo was examined in gastric cancer and adjacent normal tissues by RT-PCR. Circ_0000190 expression in gastric cancer cell lines was detected by FISH and RT-PCR. The role of the circRNA in gastric cancer cells was assessed by the analysis of cell viability, apoptosis, proliferation, cell cycle and migration. The potential effector of circ_0000190 was predicted by computational screen and validated by luciferase reporter assay. Furthermore, Mice model of human gastric cancer: was established to observe the underlying mechanisms of circ_0000190.Results: Circ_0000190 was down-regulated in gastric cancer tissues and cells, with a major location in cytoplasm. Circ_0000190 inhibited gastric cancer cell viability, proliferation and migration, and induced apoptosis and cell cycle arrest by regulating the expression of capase-3, p27 and cyclin D. In addition, the circRNA was validated as a sponge of miR-1252, which directly targeted PAK3. The effects of circ_0000190 on the cellular processes were blocked by miR-1252 mimics, which could be rescued after further overexpression of PAK3.Conclusions: Circ_0000190 suppresses gastric cancer progression potentially via inhibiting miR-1252/PAK3 pathway, employing circ_0000190 might be a promising therapeutic strategy for the treatment of gastric cancer.

Effect of Chrysanthemum flavonoids on growth and cell cycle regulators of gastric cancer cell

2010 ◽  
Vol 34 (8) ◽  
pp. S50-S50
Author(s):  
Xiaoyan Pan ◽  
Xinmei Zhou ◽  
Guangtao Xu ◽  
Lingfen Miao ◽  
Shuoru Zhu
Keyword(s):  
Gastric Cancer ◽  
Cell Cycle ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Cell Cycle Regulators
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