An in vivo method for recording single unit activity in lumbar spinal cord in mice anesthetized with a volatile anesthetic

2004 ◽  
Vol 13 (2) ◽  
pp. 126-134 ◽  
Author(s):  
Jason M Cuellar ◽  
Joseph F Antognini ◽  
Earl Carstens
Keyword(s):  
Spinal Cord ◽  
Unit Activity ◽  
Single Unit ◽  
Volatile Anesthetic ◽  
Lumbar Spinal Cord ◽  
Single Unit Activity ◽  
Lumbar Spinal

scholarly journals A method for recording single unit activity in lumbar spinal cord in rats anesthetized with nitrous oxide in a hyperbaric chamber

2007 ◽  
Vol 160 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Joseph F. Antognini ◽  
Richard J. Atherley ◽  
Michael J. Laster ◽  
Earl Carstens ◽  
Robert C. Dutton ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Nitrous Oxide ◽  
Unit Activity ◽  
Single Unit ◽  
Lumbar Spinal Cord ◽  
Single Unit Activity ◽  
Hyperbaric Chamber ◽  
Lumbar Spinal

Spinal Unit Activity During Synchronization of a Convulsive Type (Strychnine Tetanus)

1956 ◽  
Vol 186 (2) ◽  
pp. 263-270 ◽  
Author(s):  
Carlo A. Terzuolo ◽  
Bo E. Gernandt
Keyword(s):  
Spinal Cord ◽  
Intravenous Injection ◽  
Unit Activity ◽  
Single Unit ◽  
Slow Waves ◽  
Single Unit Activity ◽  
Oscillatory Potential ◽  
Convulsive Activity ◽  
Decerebrate Cat ◽  
The Relationship

Single unit activity during convulsive synchronized discharge and the relationship between unit activity and slow ‘waves’ were studied in the spinal cord of the decerebrate cat after intravenous injection of strychnine. Steel needle microelectrodes were used. Units did discharge in burst (from 1 to 5 impulses) only in coincidence with the slow oscillatory potential (tetanic ‘waves’). The firing is confined to the rising phase of this potential. Changes in d.c. state related to a convulsive activity were also studied. A relationship was found between frequency of the tetanic rhythm and d.c. state of spinal structures. The mechanisms of synchronization and rhythmicity of the convulsive activity in a population of neurons are discussed in connection with the problem of interaction between units.

scholarly journals In Vivo Recording of Single-Unit Activity during Singing in Zebra Finches

2014 ◽  
Vol 2014 (12) ◽  
pp. pdb.prot084624 ◽  
Author(s):  
Tatsuo S. Okubo ◽  
Emily L. Mackevicius ◽  
Michale S. Fee
Keyword(s):  
Unit Activity ◽  
Single Unit ◽  
Zebra Finches ◽  
Single Unit Activity

Active Dendritic Integration of Inhibitory Synaptic Inputs In Vivo

2003 ◽  
Vol 90 (6) ◽  
pp. 3617-3624 ◽  
Author(s):  
Jason J. Kuo ◽  
Robert H. Lee ◽  
Michael D. Johnson ◽  
Heather M. Heckman ◽  
C. J. Heckman
Keyword(s):  
Spinal Cord ◽  
Lumbar Spinal Cord ◽  
Standard State ◽  
Dendritic Integration ◽  
Local Inhibition ◽  
Highly Active ◽  
Linear Integration ◽  
Inward Currents ◽  
Lumbar Spinal

Synaptic integration in vivo often involves activation of many afferent inputs whose firing patterns modulate over time. In spinal motoneurons, sustained excitatory inputs undergo enormous enhancement due to persistent inward currents (PICs) that are generated primarily in the dendrites and are dependent on monoaminergic neuromodulatory input from the brain stem to the spinal cord. We measured the interaction between dendritic PICs and inhibition generated by tonic electrical stimulation of nerves to antagonist muscles during voltage clamp in motoneurons in the lumbar spinal cord of the cat. Separate samples of cells were obtained for two different states of monoaminergic input: standard (provided by the decerebrate preparation, which has tonic activity in monoaminergic axons) and minimal (the chloralose anesthetized preparation, which lacks tonic monoaminergic input). In the standard state, steady inhibition that increased the input conductance of the motoneurons by an average of 38% reduced the PIC by 69%. The range of this reduction, from <10% to >100%, was proportional to the magnitude of the applied inhibition. Thus nearly linear integration of synaptic inhibition may occur in these highly active dendrites. In the minimal state, PICs were much smaller, being approximately equal to inhibition-suppressed PICs in the standard state. Inhibition did not further reduce these already small PICs. Overall, these results demonstrate that inhibition from local spinal circuits can oppose the facilitation of dendritic PICs by descending monoaminergic inputs. As a result, local inhibition may also suppress active dendritic integration of excitatory inputs.

scholarly journals Characterization of DTI Indices in the Cervical, Thoracic, and Lumbar Spinal Cord in Healthy Humans

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Rachael L. Bosma ◽  
Patrick W. Stroman
Keyword(s):  
Spinal Cord ◽  
Mean Diffusivity ◽  
Lumbar Spinal Cord ◽  
Single Shot ◽  
Tissue Properties ◽  
Healthy Humans ◽  
Diffusion Weighted Images ◽  
Lumbar Spinal

The aim of this study was to characterizein vivomeasurements of diffusion along the length of the entire healthy spinal cord and to compare DTI indices, including fractional anisotropy (FA) and mean diffusivity (MD), between cord regions. The objective is to determine whether or not there are significant differences in DTI indices along the cord that must be considered for future applications of characterizing the effects of injury or disease. A cardiac gated, single-shot EPI sequence was used to acquire diffusion-weighted images of the cervical, thoracic, and lumbar regions of the spinal cord in nine neurologically intact subjects (19 to 22 years). For each cord section, FA versus MD values were plotted, and a k-means clustering method was applied to partition the data according to tissue properties. FA and MD values from both white matter (averageFA=0.69, averageMD=0.93×10−3 mm2/s) and grey matter (averageFA=0.44, averageMD=1.8×10−3 mm2/s) were relatively consistent along the length of the cord.

scholarly journals In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats

2021 ◽  
Vol 22 (7) ◽  
pp. 3400
Author(s):  
Daisuke Uta ◽  
Takumi Oti ◽  
Tatsuya Sakamoto ◽  
Hirotaka Sakamoto
Keyword(s):  
Spinal Cord ◽  
Electrical Response ◽  
Oxytocin Receptor ◽  
Lumbar Spinal Cord ◽  
Whole Cell ◽  
Optogenetic Stimulation ◽  
Lumbar Spinal ◽  
Whole Cell Recordings ◽  
Stimulation Of

The spinal ejaculation generator (SEG) is located in the central gray (lamina X) of the rat lumbar spinal cord and plays a pivotal role in the ejaculatory reflex. We recently reported that SEG neurons express the oxytocin receptor and are activated by oxytocin projections from the paraventricular nucleus of hypothalamus (PVH). However, it is unknown whether the SEG responds to oxytocin in vivo. In this study, we analyzed the characteristics of the brain–spinal cord neural circuit that controls male sexual function using a newly developed in vivo electrophysiological technique. Optogenetic stimulation of the PVH of rats expressing channel rhodopsin under the oxytocin receptor promoter increased the spontaneous firing of most lamina X SEG neurons. This is the first demonstration of the in vivo electrical response from the deeper (lamina X) neurons in the spinal cord. Furthermore, we succeeded in the in vivo whole-cell recordings of lamina X neurons. In vivo whole-cell recordings may reveal the features of lamina X SEG neurons, including differences in neurotransmitters and response to stimulation. Taken together, these results suggest that in vivo electrophysiological stimulation can elucidate the neurophysiological response of a variety of spinal neurons during male sexual behavior.

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