Manipulating Wnt signaling at different subcellular levels affects the fate of neonatal neural stem/progenitor cells

2016 ◽  
Vol 1651 ◽  
pp. 73-87 ◽  
Author(s):  
Jan Kriska ◽  
Pavel Honsa ◽  
David Dzamba ◽  
Olena Butenko ◽  
Denisa Kolenicova ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Progenitor Cells ◽  
Neural Stem Progenitor Cells

scholarly journals Glia and Neural Stem and Progenitor Cells of the Healthy and Ischemic Brain: The Workplace for the Wnt Signaling Pathway

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 804
Author(s):  
Tomas Knotek ◽  
Lucie Janeckova ◽  
Jan Kriska ◽  
Vladimir Korinek ◽  
Miroslava Anderova
Keyword(s):  
Wnt Signaling ◽  
Progenitor Cells ◽  
Current Knowledge ◽  
Wnt Signaling Pathway ◽  
Cell Types ◽  
Negative Effects ◽  
Future Directions ◽  
Ischemic Brain ◽  
Stem And Progenitor Cells ◽  
Neural Stem Progenitor Cells

Wnt signaling plays an important role in the self-renewal, fate-commitment and survival of the neural stem/progenitor cells (NS/PCs) of the adult central nervous system (CNS). Ischemic stroke impairs the proper functioning of the CNS and, therefore, active Wnt signaling may prevent, ameliorate, or even reverse the negative effects of ischemic brain injury. In this review, we provide the current knowledge of Wnt signaling in the adult CNS, its status in diverse cell types, and the Wnt pathway’s impact on the properties of NS/PCs and glial cells in the context of ischemic injury. Finally, we summarize promising strategies that might be considered for stroke therapy, and we outline possible future directions of the field.

scholarly journals Wnt/β-Catenin Signaling Promotes Differentiation of Ischemia-Activated Adult Neural Stem/Progenitor Cells to Neuronal Precursors

2021 ◽  
Vol 15 ◽  
Author(s):  
Jan Kriska ◽  
Lucie Janeckova ◽  
Denisa Kirdajova ◽  
Pavel Honsa ◽  
Tomas Knotek ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Progenitor Cells ◽  
Wnt Pathway ◽  
Cultured Cells ◽  
Focal Cerebral Ischemia ◽  
Cell Types ◽  
Adult Brain ◽  
Negative Consequences ◽  
Neural Stem Progenitor Cells ◽  
The Impact

Modulating endogenous regenerative processes may represent a suitable treatment for central nervous system (CNS) injuries, such as stroke or trauma. Neural stem/progenitor cells (NS/PCs), which naturally reside in the subventricular zone (SVZ) of the adult brain, proliferate and differentiate to other cell types, and therefore may compensate the negative consequences of ischemic injury. The fate of NS/PCs in the developing brain is largely influenced by Wingless/Integrated (Wnt) signaling; however, its role in the differentiation of adult NS/PCs under ischemic conditions is still enigmatic. In our previous study, we identified the Wnt/β-catenin signaling pathway as a factor promoting neurogenesis at the expense of gliogenesis in neonatal mice. In this study, we used adult transgenic mice in order to assess the impact of the canonical Wnt pathway modulation (inhibition or hyper-activation) on NS/PCs derived from the SVZ, and combined it with the middle cerebral artery occlusion (MCAO) to disclose the effect of focal cerebral ischemia (FCI). Based on the electrophysiological properties of cultured cells, we first identified three cell types that represented in vitro differentiated NS/PCs – astrocytes, neuron-like cells, and precursor cells. Following FCI, we detected fewer neuron-like cells after Wnt signaling inhibition. Furthermore, the immunohistochemical analysis revealed an overall higher expression of cell-type-specific proteins after FCI, indicating increased proliferation and differentiation rates of NS/PCs in the SVZ. Remarkably, Wnt signaling hyper-activation increased the abundance of proliferating and neuron-like cells, while Wnt pathway inhibition had the opposite effect. Finally, the expression profiling at the single cell level revealed an increased proportion of neural stem cells and neuroblasts after FCI. These observations indicate that Wnt signaling enhances NS/PCs-based regeneration in the adult mouse brain following FCI, and supports neuronal differentiation in the SVZ.

Wnt signaling pathway regulates the differentiation of hepatic progenitor cells

2010 ◽  
Vol 34 (8) ◽  
pp. S41-S41
Author(s):  
Yang Bi ◽  
Yun He ◽  
Tingyu Li ◽  
Tao Feng ◽  
Tongchuan He
Keyword(s):  
Wnt Signaling ◽  
Progenitor Cells ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Hepatic Progenitor Cells

scholarly journals Epigenetic regulation of the stem cell mitogen Fgf-2 by Mbd1 in adult neural stem/progenitor cells. VOLUME 283 (2008) PAGES 27644-27652

2009 ◽  
Vol 284 (13) ◽  
pp. 8995
Author(s):  
Xuekun Li ◽  
Basam Z. Barkho ◽  
Jinfeng Bao ◽  
Yuping Luo ◽  
Richard D. Smrt ◽  
...  
Keyword(s):  
Stem Cell ◽  
Progenitor Cells ◽  
Epigenetic Regulation ◽  
Neural Stem Progenitor Cells ◽  
Cell Mitogen
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