Label-free, real-time on-chip sensing of living cells via grating-coupled surface plasmon resonance

2019 ◽  
Vol 254 ◽  
pp. 106262 ◽  
Author(s):  
Giulia Borile ◽  
Stefano Rossi ◽  
Andrea Filippi ◽  
Enrico Gazzola ◽  
Pietro Capaldo ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Living Cells ◽  
Label Free ◽  
On Chip

scholarly journals Label-free, real-time on-chip sensing of living cancer cell via grating-coupled surface plasmon resonance

2019 ◽  
Author(s):  
Giulia Borile ◽  
Stefano Rossi ◽  
Andrea Filippi ◽  
Enrico Gazzola ◽  
Pietro Capaldo ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cell Number ◽  
Label Free ◽  
Cellular Processes ◽  
Spr Sensor ◽  
Microfluidic Chamber ◽  
On Chip

ABSTRACTThe application of nanotechnologies to address biomedical questions is a key strategy for innovation in biomedical research. Among others, a key point consists in the availability of nanotechnologies for monitoring cellular processes in a real-time and label-free approach. Here, we focused on a grating-coupled Surface Plasmon Resonance (GC-SPR) sensor exploiting phase interrogation. This sensor can be integrated in a microfluidic chamber that ensures cell viability and avoids cell stress. We report the calibration of the sensor response as a function of cell number and its application to monitor cell adhesion kinetics as well as cell response to an external stimulus. Our results show that GC-SPR sensors can offer a valuable alternative to prism-coupled or imaging SPR devices, amenable for microfluidic implementation.

scholarly journals Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 102 ◽  
Author(s):  
Richard Schasfoort ◽  
Fikri Abali ◽  
Ivan Stojanovic ◽  
Gestur Vidarsson ◽  
Leon Terstappen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mammalian Cells ◽  
Living Cells ◽  
Label Free ◽  
Intact Cells ◽  
Cell Parameters ◽  
Spr Imaging ◽  
Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

Surface plasmon resonance imaging for real-time, label-free analysis of protein interactions with carbohydrate microarrays

2007 ◽  
Vol 25 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Rositsa Karamanska ◽  
Jonathan Clarke ◽  
Ola Blixt ◽  
James I. MacRae ◽  
Jiquan Q. Zhang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Protein Interactions ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging

scholarly journals Surface plasmon resonance label-free monitoring of antibody antigen interactions in real time

2007 ◽  
Vol 35 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Asta Kausaite ◽  
Martijn van Dijk ◽  
Jan Castrop ◽  
Almira Ramanaviciene ◽  
John P. Baltrus ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free

scholarly journals Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation

2014 ◽  
Vol 50 (27) ◽  
pp. 3585-3588 ◽  
Author(s):  
Laura G. Carrascosa ◽  
Abu Ali Ibn Sina ◽  
Ramkumar Palanisamy ◽  
Borja Sepulveda ◽  
Marinus A. Otte ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free ◽  
Real Time Detection ◽  
Molecular Inversion Probe ◽  
Specific Label

DNA regional methylation can be detected in real-time and label-free using surface plasmon resonance biosensing coupled to molecular inversion probe based amplification.

Amphetamine Detection Based on Surface Plasmon Resonance Techniques

2010 ◽  
Vol 143-144 ◽  
pp. 1056-1058 ◽  
Author(s):  
Yu Bao ◽  
Bo Li ◽  
Yi Ran Guan ◽  
Gui Fu Yang
Keyword(s):  
Molecular Structure ◽  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Sensitivity ◽  
Experimental Results ◽  
New Method ◽  
Label Free

This paper introduced a new method for detection of amphetamine based on the Surface Plasmon Resonance (SPR) techniques. Experimental results show that SPR is approved to be a suitable approach for detection of amphetamine due to its unique properties such as label-free, real-time, high sensitivity, etc. By introducing such a SPR detection, 10μg/ml amphetamine could be easily detected and compounds with similar molecular structure are also expected suitable for SPR detection.

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