scholarly journals Surface plasmon resonance label-free monitoring of antibody antigen interactions in real time

2007 ◽  
Vol 35 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Asta Kausaite ◽  
Martijn van Dijk ◽  
Jan Castrop ◽  
Almira Ramanaviciene ◽  
John P. Baltrus ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free

Surface plasmon resonance imaging for real-time, label-free analysis of protein interactions with carbohydrate microarrays

2007 ◽  
Vol 25 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Rositsa Karamanska ◽  
Jonathan Clarke ◽  
Ola Blixt ◽  
James I. MacRae ◽  
Jiquan Q. Zhang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Protein Interactions ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging

scholarly journals Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation

2014 ◽  
Vol 50 (27) ◽  
pp. 3585-3588 ◽  
Author(s):  
Laura G. Carrascosa ◽  
Abu Ali Ibn Sina ◽  
Ramkumar Palanisamy ◽  
Borja Sepulveda ◽  
Marinus A. Otte ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free ◽  
Real Time Detection ◽  
Molecular Inversion Probe ◽  
Specific Label

DNA regional methylation can be detected in real-time and label-free using surface plasmon resonance biosensing coupled to molecular inversion probe based amplification.

Amphetamine Detection Based on Surface Plasmon Resonance Techniques

2010 ◽  
Vol 143-144 ◽  
pp. 1056-1058 ◽  
Author(s):  
Yu Bao ◽  
Bo Li ◽  
Yi Ran Guan ◽  
Gui Fu Yang
Keyword(s):  
Molecular Structure ◽  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Sensitivity ◽  
Experimental Results ◽  
New Method ◽  
Label Free

This paper introduced a new method for detection of amphetamine based on the Surface Plasmon Resonance (SPR) techniques. Experimental results show that SPR is approved to be a suitable approach for detection of amphetamine due to its unique properties such as label-free, real-time, high sensitivity, etc. By introducing such a SPR detection, 10μg/ml amphetamine could be easily detected and compounds with similar molecular structure are also expected suitable for SPR detection.

scholarly journals Surface plasmon resonance: principles, methods and applications in biomedical sciences

2003 ◽  
Vol 17 (2-3) ◽  
pp. 255-273 ◽  
Author(s):  
Patrick Englebienne ◽  
Anne Van Hoonacker ◽  
Michel Verhas
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Metal Surface ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Colloidal Gold ◽  
Biomedical Applications ◽  
Clinical Chemistry ◽  
Label Free

Surface plasmon resonance (SPR) is a phenomenon occuring at metal surfaces (typically gold and silver) when an incident light beam strikes the surface at a particular angle. Depending on the thickness of a molecular layer at the metal surface, the SPR phenomenon results in a graded reduction in intensity of the reflected light. Biomedical applications take advantage of the exquisite sensitivity of SPR to the refractive index of the medium next to the metal surface, which makes it possible to measure accurately the adsorption of molecules on the metal surface and their eventual interactions with specific ligands. The last ten years have seen a tremendous development of SPR use in biomedical applications. The technique is applied not only to the measurement in real-time of the kinetics of ligand–receptor interactions and to the screening of lead compounds in the pharmaceutical industry, but also to the measurement of DNA hybridization, enzyme–substrate interactions, in polyclonal antibody characterization, epitope mapping, protein conformation studies and label-free immunoassays. Conventional SPR is applied in specialized biosensing instruments. These instruments use expensive sensor chips of limited reuse capacity and require complex chemistry for ligand or protein immobilization. Our laboratory has successfully applied SPR with colloidal gold particles in buffered solution. This application offers many advantages over conventional SPR. The support is cheap, easily synthesized, and can be coated with various proteins or protein–ligand complexes by charge adsorption. With colloidal gold, the SPR phenomenon can be monitored in any UV-vis spectrophotometer. For high‒throughput applications, we have adapted the technology in an automated clinical chemistry analyzer. This simple technology finds application in label-free quantitative immunoassay techniques for proteins and small analytes, in conformational studies with proteins as well as in the real-time association-dissociation measurements of receptor–ligand interactions, for high-throughput screening and lead optimization.

scholarly journals Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior

2014 ◽  
Vol 56 ◽  
pp. 359-367 ◽  
Author(s):  
Yanina Shevchenko ◽  
Gulden Camci-Unal ◽  
Davide F. Cuttica ◽  
Mehmet R. Dokmeci ◽  
Jacques Albert ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Fiber Sensor ◽  
Label Free ◽  
Cellular Behavior

scholarly journals Label-free, real-time on-chip sensing of living cancer cell via grating-coupled surface plasmon resonance

2019 ◽  
Author(s):  
Giulia Borile ◽  
Stefano Rossi ◽  
Andrea Filippi ◽  
Enrico Gazzola ◽  
Pietro Capaldo ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cell Number ◽  
Label Free ◽  
Cellular Processes ◽  
Spr Sensor ◽  
Microfluidic Chamber ◽  
On Chip

ABSTRACTThe application of nanotechnologies to address biomedical questions is a key strategy for innovation in biomedical research. Among others, a key point consists in the availability of nanotechnologies for monitoring cellular processes in a real-time and label-free approach. Here, we focused on a grating-coupled Surface Plasmon Resonance (GC-SPR) sensor exploiting phase interrogation. This sensor can be integrated in a microfluidic chamber that ensures cell viability and avoids cell stress. We report the calibration of the sensor response as a function of cell number and its application to monitor cell adhesion kinetics as well as cell response to an external stimulus. Our results show that GC-SPR sensors can offer a valuable alternative to prism-coupled or imaging SPR devices, amenable for microfluidic implementation.

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