Insights into cell penetrating peptide conjugated gold nanoparticles for internalization into bacterial cells

2018 ◽  
Vol 237 ◽  
pp. 38-46 ◽  
Author(s):  
Munish Kumar ◽  
Werner Tegge ◽  
Nishima Wangoo ◽  
Rahul Jain ◽  
Rohit K. Sharma
Keyword(s):  
Gold Nanoparticles ◽  
Cell Penetrating Peptide ◽  
Bacterial Cells ◽  
Cell Penetrating

scholarly journals Synthesis and Study of Cell-Penetrating Peptide-Modified Gold Nanoparticles [Corrigendum]

2021 ◽  
Vol Volume 16 ◽  
pp. 4177-4178
Author(s):  
Félix Boussoufi ◽  
Sandra Navarro Gallón ◽  
Run Chang ◽  
Thomas Webster
Keyword(s):  
Gold Nanoparticles ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

Cell-Penetrating Peptide-Modified Gold Nanoparticles for the Delivery of Doxorubicin to Brain Metastatic Breast Cancer

2016 ◽  
Vol 13 (6) ◽  
pp. 1843-1854 ◽  
Author(s):  
Ramin A. Morshed ◽  
Megan E. Muroski ◽  
Qing Dai ◽  
Michelle L. Wegscheid ◽  
Brenda Auffinger ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Metastatic Breast Cancer ◽  
Metastatic Breast ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

scholarly journals Synthesis and study of cell-penetrating peptide-modified gold nanoparticles

2018 ◽  
Vol Volume 13 ◽  
pp. 6199-6205 ◽  
Author(s):  
Félix Boussoufi ◽  
Sandra Navarro Gallón ◽  
Run Chang ◽  
Thomas Webster
Keyword(s):  
Gold Nanoparticles ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

scholarly journals Synthesis and Enhanced Cellular Uptake In Vitro of Anti-HER2 Multifunctional Gold Nanoparticles

Cancers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 870 ◽  
Author(s):  
Cruz ◽  
Kayser
Keyword(s):  
Gold Nanoparticles ◽  
Cellular Uptake ◽  
Therapeutic Index ◽  
Active Targeting ◽  
Cell Penetrating Peptide ◽  
Antibody Drug Conjugate ◽  
Drug Conjugates ◽  
Cell Penetrating ◽  
Antibody Drug

Nanoparticle carriers offer the possibility of enhanced delivery of therapeutic payloads in tumor tissues due to tumor-selective accumulation through the enhanced permeability and retention effect (EPR). Gold nanoparticles (AuNP), in particular, possess highly appealing features for development as nanomedicines, such as biocompatibility, tunable optical properties and a remarkable ease of surface functionalization. Taking advantage of the latter, several strategies have been designed to increase treatment specificity of gold nanocarriers by attaching monoclonal antibodies on the surface, as a way to promote selective interactions with the targeted cells—an approach referred to as active-targeting. Here, we describe the synthesis of spherical gold nanoparticles surface-functionalized with an anti-HER2 antibody-drug conjugate (ADC) as an active targeting agent that carries a cytotoxic payload. In addition, we enhanced the intracellular delivery properties of the carrier by attaching a cell penetrating peptide to the active-targeted nanoparticles. We demonstrate that the antibody retains high receptor-affinity after the structural modifications performed for drug-conjugation and nanoparticle attachment. Furthermore, we show that antibody attachment increases cellular uptake in HER2 amplified cell lines selectively, and incorporation of the cell penetrating peptide leads to a further increase in cellular internalization. Nanoparticle-bound antibody-drug conjugates retain high antimitotic potency, which could contribute to a higher therapeutic index in high EPR tumors.

An Effort to Making a Colorimitric Nano-Biosensor for Vibrio cholera Detection

2020 ◽  
Vol 16 (5) ◽  
pp. 793-804
Author(s):  
Naimeh Mahheidari ◽  
Jamal Rashidiani ◽  
Hamid Kooshki ◽  
Khadijeh Eskandari
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
Au Nanoparticle ◽  
Bacteria Detection ◽  
Great Promise ◽  
Bacterial Cells ◽  
Vibrio Cholera ◽  
Minimum Concentration ◽  
Vis Spectroscopy ◽  
Fast Procedure

Background: Today, nanoparticles hold great promise in biomedical researches and applications including bacteria detection. The rapid and sensitive outcomes of bacteria detection strategies using nanoparticle conjugates become determinative, especially in bacterial outbreaks. In the current research, we focused on detecting V. cholera bacteria and its toxin using a thiocyanate/Au nanoparticle. Thiocyanate adsorbed strongly on the surface of gold nanoparticles and changed the surface by enhancing surface plasmon resonance of gold nanoparticles. Objective: This method is tried to introduce a simple and fast procedure to assay vibrio cholera. So, it is observed by the naked eyes as well. Methods: We used two antibodies (Ab) for V. cholera detection: a) a primary antibody conjugated to magnetic nanoparticles (MNPs) for trapping V. cholera bacterial cells, and b) a secondary Abconjugated thiocyanate-GNPs as a colorimetric detector. Then, an immuno-magnetic separation system connected to a colorimetric assay was designed based on the GNPs. The results were measured by ultraviolet-visible (UV-Vis) spectroscopy. Results: The results showed that gold nanoparticles are an appropriate optical assay for detecting biological samples in a minimum concentration and also it can be easily seen by the naked eyes. The linear range of this biosensor is 3.2×104 to 28×104 cells per ml. Conclusion: In this research, a colorimetric immune assay based on gold nanoparticles was designed to improve the sensitivity of V. cholera detection. Also, this method can be used for the detection of other biological agents.

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