scholarly journals Microstructural and compositional contributions towards the mechanical behavior of aging human bone measured by cyclic and impact reference point indentation

Bone ◽  
2016 ◽  
Vol 87 ◽  
pp. 37-43 ◽  
Author(s):  
Adam C. Abraham ◽  
Avinesh Agarwalla ◽  
Aditya Yadavalli ◽  
Jenny Y. Liu ◽  
Simon Y. Tang
Keyword(s):  
Mechanical Behavior ◽  
Reference Point ◽  
Human Bone ◽  
Reference Point Indentation

scholarly journals Determination of the Depth- and Time- Dependent Mechanical Behavior of Mouse Articular Cartilage Using Cyclic Reference Point Indentation

Cartilage ◽  
2018 ◽  
Vol 11 (3) ◽  
pp. 358-363 ◽  
Author(s):  
Andrew Chang ◽  
Simon Y. Tang
Keyword(s):  
Articular Cartilage ◽  
Mechanical Behavior ◽  
Mouse Models ◽  
Reference Point ◽  
Molecular Mechanisms ◽  
Cartilage Degeneration ◽  
Small Scale ◽  
Superficial Zone ◽  
Vast Number ◽  
Reference Point Indentation

Mouse models of osteoarthritis and cartilage degeneration are important and powerful tools for investigating the molecular mechanisms of the disease pathology. Because of the vast number of genetically modified mouse models that are available for research, the ability to use these models is particularly attractive for the mechanobiologic interactions in the pathogenesis of osteoarthritis. However, the very small scale of mouse articular cartilage, where the healthy tissue is only 80 µm in thickness, poses challenges in quantifying mechanical characteristics of the tissue. We introduce here a novel approach that combines experimental and analytical methods to quantify the nuanced mechanical changes during cartilage degeneration at this scale. Cyclic reference point indentation is used to directly test the murine articular cartilage to obtain the force-deformation and the phase-shift characteristics of the tissue. The cartilage zonal thicknesses are confirmed from histology. These data are then fitted to a parallel spring model to determine the depth-dependent tissue stiffness and modulus. Using this approach, we investigated the effects of trypsin degradation on the zonal mechanical behavior of mouse articular cartilage. We observe a decline of the superficial zone stiffness coupled with the loss of the superficial layer. Subsequent degradation by trypsin allowed the identification of middle- and deep- zone properties. Taken together, this approach can be a useful tool for understanding the disease mechanisms of cartilage homeostasis and degeneration, and for monitoring of therapies for osteoarthritis.

Bone material properties as measured by Reference Point Indentation are low in subjects with acromegaly

2016 ◽  
Author(s):  
Frank Malgo ◽  
Neveen A T Hamdy ◽  
Alberto M Pereira ◽  
Nienke R Biermasz ◽  
Natasha M Appelman-Dijkstra
Keyword(s):  
Material Properties ◽  
Reference Point ◽  
Bone Material ◽  
Bone Material Properties ◽  
Reference Point Indentation

scholarly journals Microhardness distribution of the tibial diaphysis and test site selection for reference point indentation technique

Medicine ◽  
2019 ◽  
Vol 98 (29) ◽  
pp. e16523 ◽  
Author(s):  
Jianzhao Wang ◽  
Bing Yin ◽  
Guobin Liu ◽  
Sheng Li ◽  
Xiaojuan Zhang ◽  
...  
Keyword(s):  
Site Selection ◽  
Reference Point ◽  
Test Site ◽  
Indentation Technique ◽  
Tibial Diaphysis ◽  
Microhardness Distribution ◽  
Reference Point Indentation ◽  
Selection For

scholarly journals Comparison of cyclic and impact-based reference point indentation measurements in human cadaveric tibia

Bone ◽  
2018 ◽  
Vol 106 ◽  
pp. 90-95 ◽  
Author(s):  
Lamya Karim ◽  
Miranda Van Vliet ◽  
Mary L. Bouxsein
Keyword(s):  
Reference Point ◽  
Reference Point Indentation

scholarly journals Determination of Elastic Modulus in Mouse Bones Using a Nondestructive Micro-Indentation Technique Using Reference Point Indentation

2018 ◽  
Vol 140 (7) ◽  
Author(s):  
Ganesh Thiagarajan ◽  
Mark T. Begonia ◽  
Mark Dallas ◽  
Nuria Lara-Castillo ◽  
JoAnna M. Scott ◽  
...  
Keyword(s):  
Elastic Modulus ◽  
Reference Point ◽  
Ex Vivo ◽  
Numerical Procedure ◽  
Indentation Modulus ◽  
Three Point Bending ◽  
Bending Modulus ◽  
Reference Point Indentation

The determination of the elastic modulus of bone is important in studying the response of bone to loading and is determined using a destructive three-point bending method. Reference point indentation (RPI), with one cycle of indentation, offers a nondestructive alternative to determine the elastic modulus. While the elastic modulus could be determined using a nondestructive procedure for ex vivo experiments, for in vivo testing, the three-point bending technique may not be practical and hence RPI is viewed as a potential alternative and explored in this study. Using the RPI measurements, total indentation distance (TID), creep indentation distance, indentation force, and the unloading slope, we have developed a numerical analysis procedure using the Oliver–Pharr (O/P) method to estimate the indentation elastic modulus. Two methods were used to determine the area function: (1) Oliver–Pharr (O/P—based on a numerical procedure) and (2) geometric (based on the calculation of the projected area of indentation). The indentation moduli of polymethyl methacrylate (PMMA) calculated by the O/P (3.49–3.68 GPa) and geometric (3.33–3.49 GPa) methods were similar to values in literature (3.5–4 GPa). In a study using femurs from C57Bl/6 mice of different ages and genders, the three-point bending modulus was lower than the indentation modulus. In femurs from 4 to 5 months old TOPGAL mice, we found that the indentation modulus from the geometric (5.61 ± 1.25 GPa) and O/P (5.53 ± 1.27 GPa) methods was higher than the three-point bending modulus (5.28 ± 0.34 GPa). In females, the indentation modulus from the geometric (7.45 ± 0.86 GPa) and O/P (7.46 ± 0.92 GPa) methods was also higher than the three-point bending modulus (7.33 ± 1.13 GPa). We can conclude from this study that the RPI determined values are relatively close to three-point bending values.

Sign in / Sign up

Export Citation Format

Share Document