RhoA/ROCK/PTEN inhibition reverses low osteoblast proliferation on calcium phosphate apatite surface

Bone ◽  
2011 ◽  
Vol 48 ◽  
pp. S111
Author(s):  
H.-M. Kim ◽  
S. Yang ◽  
Y.-J. Lee ◽  
Y.S. Tian ◽  
F.H. Yu ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Osteoblast Proliferation ◽  
Pten Inhibition ◽  
Apatite Surface

Biomimetic Mineralization and Bioactivity of Phosphorylated Chitosan

2005 ◽  
Vol 288-289 ◽  
pp. 429-432 ◽  
Author(s):  
Zhi Qing Chen ◽  
Quan Li Li ◽  
Quan Zen ◽  
Gang Li ◽  
Hao Bin Jiang ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Crystal Nucleation ◽  
Osteoblast Proliferation ◽  
Biomimetic Mineralization ◽  
P Content ◽  
Proliferation And Differentiation ◽  
Nucleation Growth

Phosphorylated chitosans were synthesized as templates to manipulate hydroxyapatite (HA) crystal nucleation, growth and microstructure. Two kinds of insoluble phosphorylated chitosan were soaked in saturated Ca(OH)2 solution for 4 d and in 1.5× SBF (simulated body fluid) solutions for 14 d at 37 °C for biomimetic mineralization. A lower [P]-content of phosphorylated chitosan promoted greater mineralization than higher [P]-content. Phosphorylated chitosan inhibited osteoblast proliferation and differentiation in vitro, while calcium phosphate phosphorylated chitosan composites did not.

Experimental Study on the Osteoinduction of Calcium Phosphate Biomaterials In Vivo and the Capability of Supporting Osteoblast Proliferation In Vitro

2005 ◽  
Vol 288-289 ◽  
pp. 281-286 ◽  
Author(s):  
C.Y. Bao ◽  
P. Li ◽  
Yan Fei Tan ◽  
Y. Cao ◽  
Xiao Yan Lin ◽  
...  
Keyword(s):  
Experimental Study ◽  
Surface Modification ◽  
Calcium Phosphate ◽  
Treated Group ◽  
Osteoblast Proliferation ◽  
Thin Slices ◽  
Proliferation In Vitro ◽  
Ceramic Cylinder

The purpose of this study was to evaluate the response of osteoblasts to calcium phosphate with different surface modification, and to evaluate the osteoinductive capabilities of these biomaterials. 60HA/40α-TCP ceramics sintered at 1250oC was applied in this study. A ceramic cylinder with F5mm×8mm and slice with F10mm×1mm were prepared respectively. One third of the ceramics was used to form bone-like apatite (BLA), and the surface of another one third was modified with collagen. Osteoblasts (1×106/ml) were co-cultured with the three kinds of thin slices for 12h, 24h and 48h. SEM observation was applied to evaluate whether the surface modification and BLA formation could affect the attachment and proliferation of osteoblast in vitro. The three kinds of cylinder samples were implanted in dog muscle to evaluate their differences in osteoinduction. Cells grew in multi-layers and attached to the surface and proliferated well in the collagen and HA/TCP group. In the untreated and BLA precipitated groups, cells did not attach to the surface well. Osteoinduction was good in the BLA precipitated group and the amount of bone formed was higher; in the untreated group and collagen-treated group, no bone formation was observed in the tested period. This result indicated that the scaffold used in cell-materials composites in vitro and that in osteoinductive material based tissue engineering in vivo was not same.

A Novel Polymer-Synthesized Ceramic Composite Based System for Bone Repair: Osteoblast Growth on Scaffolds with Varied Calcium Phosphate Content

2004 ◽  
Vol 845 ◽  
Author(s):  
Yusuf M. Khan ◽  
Dhirendra S. Katti ◽  
Cato T. Laurencin
Keyword(s):  
Calcium Phosphate ◽  
Calcium Ion ◽  
Ceramic Composite ◽  
Bone Repair ◽  
Cell Number ◽  
Ion Concentration ◽  
Osteoblast Proliferation ◽  
3 Dimensional ◽  
Time Period ◽  
Calcium Ion Concentration

ABSTRACTPolymer/ceramic composite matrices for bone tissue engineering were constructed by synthesizing a poorly crystalline calcium phosphate within poly(lactide-co-glycolide) microspheres that were subsequently fused together to form 3-dimensional structures. Calcium ion dissolution from the composite matrices in simulated body fluid was examined over a 24 hour period. The initial 4 hour period showed an increase in calcium ion concentration, whereas, a decrease in calcium ion concentration was noted thereafter. This decrease in concentration coincided with the precipitation of calcium phosphate on the surface of the matrices. Osteoblast proliferation studies on composite matrices showed statistically significant increases in cell number throughout the 21 day time period. These data together suggest that the composite matrix acts as both a calcium ion donor for reprecipitation of calcium phosphate that may enhance osteointegration of the implant, and a suitable surface for osteoblast proliferation.

Seeding Cells on Calcium Phosphate Scaffolds Using Hydrogel Enhanced Osteoblast Proliferation and Differentiation

2013 ◽  
Vol 42 (7) ◽  
pp. 1424-1435 ◽  
Author(s):  
Min-Ho Hong ◽  
Sung-Min Kim ◽  
Ji-Yeon Om ◽  
Namyong Kwon ◽  
Yong-Keun Lee
Keyword(s):  
Calcium Phosphate ◽  
Osteoblast Proliferation ◽  
Proliferation And Differentiation

Experimental Study on the Osteoinduction of Calcium Phosphate Biomaterials In Vivo and the Capability of Supporting Osteoblast Proliferation In Vitro

2005 ◽  
Vol 284-286 ◽  
pp. 293-296 ◽  
Author(s):  
C.Y. Bao ◽  
P. Li ◽  
Yan Fei Tan ◽  
Y. Cao ◽  
X.Y Ling ◽  
...  
Keyword(s):  
Experimental Study ◽  
Surface Modification ◽  
Calcium Phosphate ◽  
Treated Group ◽  
Osteoblast Proliferation ◽  
Thin Slices ◽  
Proliferation In Vitro ◽  
Ceramic Cylinder

The purpose of this study was to evaluate the response of osteoblasts to the calcium phosphate with different surface modification, and to evaluate the osteoinductive capabilities of these biomaterials. 60HA/40α-TCP ceramics sintered at 1250oC was applied in this study. The ceramic cylinder with F5mm×8mm and slice with F10mm×1mm were prepared respectively. One thirds of the ceramics were formed bone-like apatite (BLA), and the surface of another one thirds was modified with collagen. Osteoblasts (1×106/ml) were co-cultured with the three kinds of thin slices for 12h, 24h and 48h. SEM observation was applied to evaluate if the surface modification and BLA formation could affect the attachment and proliferation of osteoblast in vitro. The three kinds of cylinder samples were implanted in dog muscle to evaluate their differences in osteoinduction. Cells grew in multi-layers and well attached to the surface and proliferated well in the group of collagen and HA/TCP. In untreated and BLA precipitated group, cells did not attach to the surface well. Osteoinduction was good in BLA precipitated group and the amount of bone formed was higher; in untreated group and collagen-treated group, no bone formation was observed in the tested period. This result indicated that the scaffold used in cell-materials composites in vitro and that in osteoinductive material based tissue engineering in vivo was not same.

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