Is it that the estrogen receptor is the only reason for osteoporosis? -- Silencing of estrogen receptor α or β does not influence the growth of human osteoblast-cell

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S396-S397
Author(s):  
Zhaozhong Wu
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Α ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell

Lecithin blended polyamide-6 high aspect ratio nanofiber scaffolds via electrospinning for human osteoblast cell culture

2011 ◽  
Vol 31 (2) ◽  
pp. 486-493 ◽  
Author(s):  
R. Nirmala ◽  
Hye-Min Park ◽  
R. Navamathavan ◽  
Hyung-Sub Kang ◽  
Mohamed H. El-Newehy ◽  
...  
Keyword(s):  
Cell Culture ◽  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Polyamide 6 ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Nanofiber Scaffolds

Activation of JNK and PAK2 is essential for citrinin-induced apoptosis in a human osteoblast cell line

2009 ◽  
Vol 24 (4) ◽  
pp. 343-356 ◽  
Author(s):  
Yu-Ting Huang ◽  
Ching-Yu Lai ◽  
Shyh-Liang Lou ◽  
Jui-Ming Yeh ◽  
Wen-Hsiung Chan
Keyword(s):  
Cell Line ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Osteoblast Cell Line ◽  
Induced Apoptosis

scholarly journals 3D Porous Calcium-Alginate Scaffolds Cell Culture System Improved Human Osteoblast Cell Clusters for Cell Therapy

Theranostics ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 643-655 ◽  
Author(s):  
Ching-Yun Chen ◽  
Cherng-Jyh Ke ◽  
Ko-Chung Yen ◽  
Hui-Chen Hsieh ◽  
Jui-Sheng Sun ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Therapy ◽  
Culture System ◽  
Calcium Alginate ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Cell Culture System ◽  
Human Osteoblast Cell ◽  
Cell Clusters

The Role of Nitric Oxide on the Proliferation of a Human Osteoblast Cell Line Stimulated With Hydroxyapatite

2008 ◽  
Vol 34 (4) ◽  
pp. 196-202 ◽  
Author(s):  
Wihaskoro Sosroseno ◽  
Erwan Sugiatno ◽  
Abdul Rani Samsudin ◽  
Mohd Fikri Ibrahim
Keyword(s):  
Nitric Oxide ◽  
Cell Proliferation ◽  
Cell Line ◽  
No Production ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Integrin Αv ◽  
Osteoblast Cell Line ◽  
Nitrite Production

Abstract The aim of the present study was to test the hypothesis that the proliferation of a human osteoblast cell line (HOS cells) stimulated with hydroxyapatite (HA) may be regulated by nitric oxide (NO). The cells were cultured on the surface of HA. Medium or cells alone were used as controls. L-arginine, D-arginine, 7-NI (an nNOS inhibitor), L-NIL (an iNOS inhibitor), L-NIO (an eNOS inhibitor) or carboxy PTIO, a NO scavenger, was added in the HA-exposed cell cultures. The cells were also precoated with anti-human integrin αV antibody. The levels of nitrite were determined spectrophotometrically. Cell proliferation was assessed by colorimetric assay. The results showed increased nitrite production and cell proliferation by HA-stimulated HOS cells up to day 3 of cultures. Anti-integrin αV antibody, L-NIO, or carboxy PTIO suppressed, but L-arginine enhanced, nitrite production and cell proliferation of HA-stimulated HOS cells. The results of the present study suggest, therefore, that interaction between HA and HOS cell surface integrin αV molecule may activate eNOS to catalyze NO production which, in turn, may regulate the cell proliferation in an autocrine fashion.

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