scholarly journals 3D Porous Calcium-Alginate Scaffolds Cell Culture System Improved Human Osteoblast Cell Clusters for Cell Therapy

Theranostics ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 643-655 ◽  
Author(s):  
Ching-Yun Chen ◽  
Cherng-Jyh Ke ◽  
Ko-Chung Yen ◽  
Hui-Chen Hsieh ◽  
Jui-Sheng Sun ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Therapy ◽  
Culture System ◽  
Calcium Alginate ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Cell Culture System ◽  
Human Osteoblast Cell ◽  
Cell Clusters

Lecithin blended polyamide-6 high aspect ratio nanofiber scaffolds via electrospinning for human osteoblast cell culture

2011 ◽  
Vol 31 (2) ◽  
pp. 486-493 ◽  
Author(s):  
R. Nirmala ◽  
Hye-Min Park ◽  
R. Navamathavan ◽  
Hyung-Sub Kang ◽  
Mohamed H. El-Newehy ◽  
...  
Keyword(s):  
Cell Culture ◽  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Polyamide 6 ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Nanofiber Scaffolds

scholarly journals Development of automatic cell culture system for cell therapy and regenerative medicine

2013 ◽  
Vol 6 (4) ◽  
pp. 189-199
Author(s):  
Shigeyuki WAKITANI ◽  
Hideaki TAHARA ◽  
Katsumi NAKASHIMA ◽  
Hitoshi HASUNUMA ◽  
Shigetaka SHIMODAIRA ◽  
...  
Keyword(s):  
Cell Culture ◽  
Regenerative Medicine ◽  
Cell Therapy ◽  
Culture System ◽  
Cell Culture System

scholarly journals In vitro analysis of bone phenotypes in Col1a1 and Jagged1 mutant mice using a standardized osteoblast cell culture system

2013 ◽  
Vol 31 (3) ◽  
pp. 293-303 ◽  
Author(s):  
Frank Thiele ◽  
Christian M. Cohrs ◽  
Gerhard K. H. Przemeck ◽  
Wolfgang Wurst ◽  
Helmut Fuchs ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Mutant Mice ◽  
Osteoblast Cell ◽  
Cell Culture System ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Development of automatic cell culture system for cell therapy and regenerative medicine

Synthesiology ◽  
2013 ◽  
Vol 6 (4) ◽  
pp. 198-208 ◽  
Author(s):  
Shigeyuki WAKITANI ◽  
Hideaki TAHARA ◽  
Katsumi NAKASHIMA ◽  
Hitoshi HASUNUMA ◽  
Shigetaka SHIMODAIRA ◽  
...  
Keyword(s):  
Cell Culture ◽  
Regenerative Medicine ◽  
Cell Therapy ◽  
Culture System ◽  
Cell Culture System

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Metabolic Diseases ◽  
Enzymatic Digestion ◽  
Cell Culture System ◽  
Culture Studies ◽  
Experimental Approaches ◽  
In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

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