Development of three-dimensional human mesenchymal stem cell tissue structures with targeted gene disruptions

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S119
Author(s):  
J. Dyson ◽  
P. Genever
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cell ◽  
Cell Tissue ◽  
Tissue Structures

scholarly journals Influence of collagen‐based integrin α 1 and α 2 mediated signaling on human mesenchymal stem cell osteogenesis in three dimensional contexts

2018 ◽  
Vol 106 (10) ◽  
pp. 2594-2604 ◽  
Author(s):  
Silvia M. Becerra‐Bayona ◽  
Viviana R. Guiza‐Arguello ◽  
Brooke Russell ◽  
Magnus Höök ◽  
Mariah S. Hahn
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cell

1002 Human urethra-engineered with human mesenchymal stem cell by newly developed scaffold-free three-dimensional cell printer

2015 ◽  
Vol 14 (2) ◽  
pp. e1002-e1002a
Author(s):  
Y. Tokunori ◽  
F. Yasuto ◽  
M. Yoshihisa ◽  
T. Yoshikazu ◽  
N. Koichi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cell ◽  
Dimensional Cell

Three-Dimensional Printing of Biomimetic Titanium Mimicking Trabecular Bone Induces Human Mesenchymal Stem Cell Proliferation

Spine ◽  
2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Stephanos Papaefstathiou ◽  
Nathanael Larochette ◽  
Rosa María Villar Liste ◽  
Esther Potier ◽  
Hervé Petite ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Trabecular Bone ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cell ◽  
Three Dimensional Printing ◽  
Stem Cell Proliferation ◽  
Dimensional Printing

scholarly journals Aspiration-assisted freeform bioprinting of mesenchymal stem cell spheroids within alginate microgels

2021 ◽  
Author(s):  
Myoung Hwan Kim ◽  
Dishary Banerjee ◽  
Nazmiye Celik ◽  
Ibrahim T Ozbolat
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Peripheral Blood Monocyte ◽  
Human Mesenchymal Stem Cell ◽  
Tissue Formation ◽  
Positional Accuracy ◽  
Bone Tissue Formation ◽  
Fusion Efficiency

Aspiration-assisted freeform bioprinting (AAfB) has emerged as a promising technique for precise placement of tissue spheroids in three-dimensional (3D) space for fabrication of tissues. For successful embedded bioprinting using AAfB, an ideal support bath should possess shear-thinning behavior and yield-stress to obtain tightly fused assembly of bioprinted spheroids. Several studies have demonstrated support baths for embedded bioprinting, but these materials pose major challenges due to their low biocompatibility, opaqueness, complex and prolonged preparation procedures, and limited spheroid fusion efficacy. In this study, to circumvent the aforementioned limitations, we present the feasibility of AAfB of human mesenchymal stem cell (hMSC) spheroids in alginate microgels as a support bath. First, alginate microgels were prepared with different particle sizes modulated by blending time and concentration, followed by determination of the optimal bioprinting conditions by the assessment of rheological properties, bioprintability, and spheroid fusion efficiency. The bioprinted and consequently self-assembled tissue structures made of hMSC spheroids were osteogenically induced for bone tissue formation. Alongside, we investigated the effects of peripheral blood monocyte-derived osteoclast incorporation into the hMSC spheroids in heterotypic bone tissue formation. We demonstrated that alginate microgels enabled unprecedented positional accuracy (~5%), transparency for visualization, and improved fusion efficiency (~97%) of bioprinted hMSC spheroids for bone fabrication. This study demonstrates the feasibility of using alginate microgels as a support bath for many different applications including but not limited to freeform bioprinting of spheroids, cell-laden hydrogels, and fugitive inks to form viable tissue constructs.

Sign in / Sign up

Export Citation Format

Share Document