Real time imaging of Gene Expression

Bone ◽  
2010 ◽  
Vol 46 ◽  
pp. S10
Author(s):  
Vasilis Ntziachristos ◽  
Vasilis Ntziachristos
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Imaging

Molecular Beacons for Sensitive Gene Detection in Living Cells

2003 ◽  
Author(s):  
Andrew Tsurkas ◽  
Gang Bao
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Molecular Beacon ◽  
Point Mutations ◽  
Living Cells ◽  
Molecular Beacons ◽  
High Signal ◽  
Gene Detection ◽  
Real Time Imaging ◽  
Background Ratio

Real-time imaging of gene expression in living cells has the potential to significantly impact clinical and laboratory studies of cancer, including cancer diagnosis and analysis. Molecular beacons (MBs) provide a simple and promising tool for the detection of target mRNA as tumor markers due to their high signal-to-background ratio, and their improved specificity in detecting point mutations. However, the harsh intracellular environment does limit the sensitivity of MB-based gene detection. Specifically, MBs bound to target mRNAs cannot be distinguished from those degraded by nucleases, or opened due to non-specific interactions. To overcome this difficulty, we have developed a novel dual FRET molecular beacons approach in which a pair of molecular beacons, one with a donor fluorophore and a second with an acceptor fluorophore, hybridize to adjacent regions on the same target resulting in fluorescence resonance energy transfer (FRET). The detection of a FRET signal leads to a substantially increased signal-to-background ratio compared with that in single molecular beacon assays and enables discrimination between fluorescence due to specific probe/target hybridization and a variety of false-positive events. We have performed systematic in-solution and cellular studies of dual FRET molecular beacon and demonstrated that this new approach allows for real-time imaging of gene expression in living cells.

scholarly journals 324. Development of Bioluminescence Tomography for Three-Dimensional Real Time Imaging of Gene Expression

2006 ◽  
Vol 13 ◽  
pp. S123-S124
Author(s):  
Nathalie Y. Toussaint ◽  
Lu Yin ◽  
Aaron Hirko ◽  
Qiang Wang ◽  
Qizhi Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Three Dimensional ◽  
Bioluminescence Tomography ◽  
Real Time Imaging

scholarly journals Characterization and Real-Time Imaging of Gene Expression of Adenovirus Embedded Silk-Elastinlike Protein Polymer Hydrogels

2008 ◽  
Vol 5 (5) ◽  
pp. 891-897 ◽  
Author(s):  
Arthur von Wald Cresce ◽  
Ramesh Dandu ◽  
Angelika Burger ◽  
Joseph Cappello ◽  
Hamidreza Ghandehari
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Imaging ◽  
Polymer Hydrogels ◽  
Protein Polymer

scholarly journals Real-time imaging of gene expression in single living cells

1998 ◽  
Vol 5 (11) ◽  
pp. R285-R290 ◽  
Author(s):  
Guy A. Rutter ◽  
Helen J. Kennedy ◽  
Christopher D. Wood ◽  
Michael R.H. White ◽  
Jeremy M. Tavaré
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Living Cells ◽  
Real Time Imaging ◽  
Single Living Cells ◽  
Single Living
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