scholarly journals 324. Development of Bioluminescence Tomography for Three-Dimensional Real Time Imaging of Gene Expression

2006 ◽  
Vol 13 ◽  
pp. S123-S124
Author(s):  
Nathalie Y. Toussaint ◽  
Lu Yin ◽  
Aaron Hirko ◽  
Qiang Wang ◽  
Qizhi Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Three Dimensional ◽  
Bioluminescence Tomography ◽  
Real Time Imaging

scholarly journals Three-dimensional real-time imaging of cardiac cell motions in living embryos

2008 ◽  
Vol 13 (1) ◽  
pp. 014006 ◽  
Author(s):  
Jian Lu ◽  
Francisco Pereira ◽  
Scott E. Fraser ◽  
Morteza Gharib
Keyword(s):  
Real Time ◽  
Three Dimensional ◽  
Cardiac Cell ◽  
Real Time Imaging

Molecular Beacons for Sensitive Gene Detection in Living Cells

2003 ◽  
Author(s):  
Andrew Tsurkas ◽  
Gang Bao
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Molecular Beacon ◽  
Point Mutations ◽  
Living Cells ◽  
Molecular Beacons ◽  
High Signal ◽  
Gene Detection ◽  
Real Time Imaging ◽  
Background Ratio

Real-time imaging of gene expression in living cells has the potential to significantly impact clinical and laboratory studies of cancer, including cancer diagnosis and analysis. Molecular beacons (MBs) provide a simple and promising tool for the detection of target mRNA as tumor markers due to their high signal-to-background ratio, and their improved specificity in detecting point mutations. However, the harsh intracellular environment does limit the sensitivity of MB-based gene detection. Specifically, MBs bound to target mRNAs cannot be distinguished from those degraded by nucleases, or opened due to non-specific interactions. To overcome this difficulty, we have developed a novel dual FRET molecular beacons approach in which a pair of molecular beacons, one with a donor fluorophore and a second with an acceptor fluorophore, hybridize to adjacent regions on the same target resulting in fluorescence resonance energy transfer (FRET). The detection of a FRET signal leads to a substantially increased signal-to-background ratio compared with that in single molecular beacon assays and enables discrimination between fluorescence due to specific probe/target hybridization and a variety of false-positive events. We have performed systematic in-solution and cellular studies of dual FRET molecular beacon and demonstrated that this new approach allows for real-time imaging of gene expression in living cells.

Wavefront Coded Imaging Systems for MEMS Analysis

2002 ◽  
Author(s):  
Daniel L. Barton ◽  
Jeremy A. Walraven ◽  
Edward R. Dowski ◽  
Rainer Danz ◽  
Andreas Faulstich ◽  
...  
Keyword(s):  
Signal Processing ◽  
Real Time ◽  
High Performance ◽  
Imaging System ◽  
Three Dimensional ◽  
Imaging Systems ◽  
Depth Of Field ◽  
Real Time Imaging ◽  
Large Depth ◽  
Wavefront Coding

Abstract A new imaging technique called Wavefront Coding allows real-time imaging of three-dimensional structures over a very large depth. Wavefront Coding systems combine aspheric optics and signal processing to achieve depth of fields ten or more times greater than that possible with traditional imaging systems. Understanding the relationships between traditional and modern imaging system design through Wavefront Coding is very challenging. In high performance imaging systems nearly all aspects of the system that could reduce image quality are carefully controlled. Modifying the optics and using signal processing can increase the amount of image information that can be recorded by microscopes. For a number of applications this increase in information can allow a single image to be used where a number of images taken at different object planes had been used before. Having very large depth of field and real-time imaging capability means that very deep structures such as surface micromachined MEMS can be clearly imaged with one image, greatly simplifying defect and failure analysis.

scholarly journals Three-dimensional real time imaging of amyloid β aggregation on living cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Masahiro Kuragano ◽  
Ryota Yamashita ◽  
Yusaku Chikai ◽  
Ryota Kitamura ◽  
Kiyotaka Tokuraku
Keyword(s):  
Real Time ◽  
Three Dimensional ◽  
Amyloid Β ◽  
Living Cells ◽  
Real Time Imaging

Errata: Three-dimensional real-time imaging of cardiac cell motions in living embryos

2008 ◽  
Vol 13 (3) ◽  
pp. 039803
Author(s):  
Jian Lu ◽  
Francisco Pereira ◽  
Scott E. Fraser ◽  
Morteza Gharib
Keyword(s):  
Real Time ◽  
Three Dimensional ◽  
Cardiac Cell ◽  
Real Time Imaging

scholarly journals Characterization and Real-Time Imaging of Gene Expression of Adenovirus Embedded Silk-Elastinlike Protein Polymer Hydrogels

2008 ◽  
Vol 5 (5) ◽  
pp. 891-897 ◽  
Author(s):  
Arthur von Wald Cresce ◽  
Ramesh Dandu ◽  
Angelika Burger ◽  
Joseph Cappello ◽  
Hamidreza Ghandehari
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Imaging ◽  
Polymer Hydrogels ◽  
Protein Polymer

Design and real-time imaging technology of three-dimensional scanning LiDAR

2019 ◽  
Vol 48 (5) ◽  
pp. 503004
Author(s):  
李小路 Li Xiaolu ◽  
曾晶晶 Zeng Jingjing ◽  
王 皓 Wang Hao ◽  
徐立军 Xu Lijun
Keyword(s):  
Real Time ◽  
Three Dimensional ◽  
Imaging Technology ◽  
Real Time Imaging ◽  
Scanning Lidar
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