High-throughput screening of ecdysone agonists using a reporter gene assay followed by 3-D QSAR analysis of the molting hormonal activity

2006 ◽  
Vol 14 (4) ◽  
pp. 1143-1159 ◽  
Author(s):  
Craig E. Wheelock ◽  
Yoshiaki Nakagawa ◽  
Toshiyuki Harada ◽  
Nobuhiro Oikawa ◽  
Miki Akamatsu ◽  
...  
Keyword(s):  
Reporter Gene ◽  
High Throughput ◽  
High Throughput Screening ◽  
Reporter Gene Assay ◽  
Hormonal Activity ◽  
Qsar Analysis ◽  
Gene Assay

scholarly journals High-throughput screening for plant defense activators using a .BETA.-glucuronidase-reporter gene assay in Arabidopsis thaliana

2009 ◽  
Vol 26 (3) ◽  
pp. 345-349 ◽  
Author(s):  
Yoshihiro Narusaka ◽  
Mari Narusaka ◽  
Hiroshi Abe ◽  
Nami Hosaka ◽  
Masatomo Kobayashi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Defense ◽  
Reporter Gene ◽  
High Throughput ◽  
High Throughput Screening ◽  
Reporter Gene Assay ◽  
Gene Assay ◽  
Glucuronidase Reporter

A Short-Incubation Reporter-Gene Assay for High-Throughput Screening of Estrogen Receptor-α Antagonists

2005 ◽  
Vol 3 (4) ◽  
pp. 393-400 ◽  
Author(s):  
Xiaoqing Shi ◽  
Wei Zheng ◽  
Jonathan E. Schneeweis ◽  
Paul A. Fischer ◽  
Berta Strulovici ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Reporter Gene ◽  
High Throughput ◽  
High Throughput Screening ◽  
Estrogen Receptor Α ◽  
Reporter Gene Assay ◽  
Gene Assay

scholarly journals Validation for high-throughput screening of a VanRS-based reporter gene assay for bacterial cell wall inhibitors

2007 ◽  
Vol 103 (1) ◽  
pp. 133-140 ◽  
Author(s):  
G. De Pascale ◽  
C. Grigoriadou ◽  
D. Losi ◽  
I. Ciciliato ◽  
M. Sosio ◽  
...  
Keyword(s):  
Cell Wall ◽  
Reporter Gene ◽  
High Throughput ◽  
Bacterial Cell ◽  
High Throughput Screening ◽  
Reporter Gene Assay ◽  
Bacterial Cell Wall ◽  
Gene Assay

High throughput screening for thyroid hormone receptor mediated effects in a newly developed gh3.tre-luc reporter gene assay

2011 ◽  
Vol 205 ◽  
pp. S161-S162
Author(s):  
D. Hernández-Moreno ◽  
J. Freitas ◽  
J.H.J. van den Berg ◽  
C. Craig-Veit ◽  
M.L. Goodson ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Reporter Gene ◽  
High Throughput ◽  
Hormone Receptor ◽  
High Throughput Screening ◽  
Thyroid Hormone Receptor ◽  
Reporter Gene Assay ◽  
Mediated Effects ◽  
Gene Assay ◽  
Luc Reporter Gene

scholarly journals Development of a Generic Dual-Reporter Gene Assay for Screening G-Protein-Coupled Receptors

2005 ◽  
Vol 10 (5) ◽  
pp. 437-446 ◽  
Author(s):  
Toby C. Kent ◽  
Kevin S. J. Thompson ◽  
Louise H. Naylor
Keyword(s):  
G Protein ◽  
Reporter Gene ◽  
High Throughput Screening ◽  
Reporter Gene Assay ◽  
G Protein Coupled Receptors ◽  
Rapid Expansion ◽  
Compound Libraries ◽  
Dual Reporter ◽  
Gene Assay ◽  
G Protein Coupled

Multiple assay formats have been developed for the pharmacological characterization of G-protein-coupled receptors (GPCRs) and for screening orphan receptors. However, the increased pace of target identification and the rapid expansion of compound libraries present the need to develop novel assay formats capable of screeningmultipleGPCRs simultaneously. To address this need, the authors have developed a generic dual-reporter gene assay that can detect ligand activity at 2 GPCRs within the same assay. Two stableHEK293 cell lineswere generated expressing either a firefly ( Photinus) luciferase gene under the control ofmultiple cAMP-response elements (CREs) or a Renillaluciferase gene under the control ofmultiple 12-Otetradecanoylphorbol-13-acetate (TPA)-responsive elements (TREs). Coseeded reporter cells were used to assess ligandbinding activity at bothGβ s-and Gβ q-coupled receptors. By selectively coexpressing receptors with a chimeric G-protein, agonist activitywas assessed atGβ i/o-coupled receptors in combinationwith eitherGβ s-or Gβ q-coupled receptors. The dual-reporter gene assaywas shown to be capable of simultaneously performing duplexed screens for a variety of agonist and/or antagonist combinations. The data generated from the duplexed reporter assays were pharmacologically relevant, and Zβ factor analysis indicated the suitability of both agonist and antagonist screens for use in high-throughput screening.

Optimization and validation of a reporter gene assay for screening of phosphodiesterase inhibitors in a high throughput system

2008 ◽  
Vol 3 (9-10) ◽  
pp. 1276-1279 ◽  
Author(s):  
Kamna Nanda ◽  
Mou Chatterjee ◽  
Ranjana Arya ◽  
Shohini Mukherjee ◽  
Kulvinder Singh Saini ◽  
...  
Keyword(s):  
Reporter Gene ◽  
High Throughput ◽  
Phosphodiesterase Inhibitors ◽  
Reporter Gene Assay ◽  
Gene Assay
Sign in / Sign up

Export Citation Format

Share Document