Optimization and validation of a reporter gene assay for screening of phosphodiesterase inhibitors in a high throughput system

2008 ◽  
Vol 3 (9-10) ◽  
pp. 1276-1279 ◽  
Author(s):  
Kamna Nanda ◽  
Mou Chatterjee ◽  
Ranjana Arya ◽  
Shohini Mukherjee ◽  
Kulvinder Singh Saini ◽  
...  
Keyword(s):  
Reporter Gene ◽  
High Throughput ◽  
Phosphodiesterase Inhibitors ◽  
Reporter Gene Assay ◽  
Gene Assay

High-throughput screening of ecdysone agonists using a reporter gene assay followed by 3-D QSAR analysis of the molting hormonal activity

2006 ◽  
Vol 14 (4) ◽  
pp. 1143-1159 ◽  
Author(s):  
Craig E. Wheelock ◽  
Yoshiaki Nakagawa ◽  
Toshiyuki Harada ◽  
Nobuhiro Oikawa ◽  
Miki Akamatsu ◽  
...  
Keyword(s):  
Reporter Gene ◽  
High Throughput ◽  
High Throughput Screening ◽  
Reporter Gene Assay ◽  
Hormonal Activity ◽  
Qsar Analysis ◽  
Gene Assay

scholarly journals High-throughput screening for plant defense activators using a .BETA.-glucuronidase-reporter gene assay in Arabidopsis thaliana

2009 ◽  
Vol 26 (3) ◽  
pp. 345-349 ◽  
Author(s):  
Yoshihiro Narusaka ◽  
Mari Narusaka ◽  
Hiroshi Abe ◽  
Nami Hosaka ◽  
Masatomo Kobayashi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Defense ◽  
Reporter Gene ◽  
High Throughput ◽  
High Throughput Screening ◽  
Reporter Gene Assay ◽  
Gene Assay ◽  
Glucuronidase Reporter

A Short-Incubation Reporter-Gene Assay for High-Throughput Screening of Estrogen Receptor-α Antagonists

2005 ◽  
Vol 3 (4) ◽  
pp. 393-400 ◽  
Author(s):  
Xiaoqing Shi ◽  
Wei Zheng ◽  
Jonathan E. Schneeweis ◽  
Paul A. Fischer ◽  
Berta Strulovici ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Reporter Gene ◽  
High Throughput ◽  
High Throughput Screening ◽  
Estrogen Receptor Α ◽  
Reporter Gene Assay ◽  
Gene Assay

scholarly journals Induction of AhR transactivation by PBDD/Fs and PCDD/Fs using a novel human-relevant, high-throughput DRhuman CALUX reporter gene assay

Chemosphere ◽  
2021 ◽  
Vol 263 ◽  
pp. 128086
Author(s):  
Clémence Budin ◽  
Harrie Besselink ◽  
Barbara M.A. van Vugt-Lussenburg ◽  
Hai-Yen Man ◽  
Bart van der Burg ◽  
...  
Keyword(s):  
Reporter Gene ◽  
High Throughput ◽  
Reporter Gene Assay ◽  
Gene Assay

scholarly journals Validation for high-throughput screening of a VanRS-based reporter gene assay for bacterial cell wall inhibitors

2007 ◽  
Vol 103 (1) ◽  
pp. 133-140 ◽  
Author(s):  
G. De Pascale ◽  
C. Grigoriadou ◽  
D. Losi ◽  
I. Ciciliato ◽  
M. Sosio ◽  
...  
Keyword(s):  
Cell Wall ◽  
Reporter Gene ◽  
High Throughput ◽  
Bacterial Cell ◽  
High Throughput Screening ◽  
Reporter Gene Assay ◽  
Bacterial Cell Wall ◽  
Gene Assay

scholarly journals Development and Use of a Gene Promoter-Based Screen to Identify Novel Inhibitors of Cyclooxygenase-2 Transcription

2001 ◽  
Vol 6 (2) ◽  
pp. 101-110 ◽  
Author(s):  
Kotha Subbaramaiah ◽  
Predrag Bulic ◽  
Yuan Lin ◽  
Andrew J. Dannenberg ◽  
David S. Pasco
Keyword(s):  
Reporter Gene ◽  
High Throughput ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Reporter Gene Assay ◽  
Cyclooxygenase 2 ◽  
Prostaglandin E ◽  
Gene Assay ◽  
Cox 2 ◽  
Stimulation Of

Cyclooxygenase-2 (COX-2) is a recognized target for cancer prevention and possibly treatment. To identify novel inhibitors of COX-2, we developed a high throughput reporter gene assay that utilizes a region of the human COX-2 promoter to drive luciferase expression. A total of 968 extracts from 266 plants were screened. Extracts from 12 plants (4.5%), including Arnebia euchroma, a medicinal plant used in the Far East to treat inflammation, inhibited the stimulation of COX-2 promoter activity. The gene promoter assay then was used to identify shikonin, a compound with known anti-inflammatory and chemopreventive properties, as an active compound in A. euchroma. To complement the gene promoter studies, we determined the effects of a mixture of shikonins on phorbol 12-myristate 13-acetate (PMA)-mediated induction of COX-2 in transformed human mammary epithelial cells. Shikonins inhibited PMA-mediated induction of COX-2 mRNA, protein, and prostaglandin E2 synthesis. In transient transfections, PMA caused a severalfold increase in COX-2 promoter activity, an effect that was suppressed by shikonins. Shikonins also inhibited PMA-mediated stimulation of extracellular signal-regulated kinasel/2 mitogen-activated protein kinases and activator protein-1 activity. Collectively, these results demonstrate the successful development and use of a high throughput reporter gene assay for the identification of a novel inhibitor of COX-2 expression.

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