scholarly journals Pathogenicity of Escherichia coli O157 in commercial broiler chickens

2018 ◽  
Vol 7 (4) ◽  
pp. 620-625 ◽  
Author(s):  
Azza A. EL-Sawah ◽  
AL Hussien M. Dahshan ◽  
El-Shaymaa El-Nahass ◽  
A.I. Abd El-Mawgoud
2019 ◽  
Vol 26 (1) ◽  
pp. 1-8
Author(s):  
Asmaa Abd Elatiff ◽  
Azza A. El-Sawah ◽  
Mohamed M. Amer ◽  
Al-Hussein M. Dahshan ◽  
Hala Salam ◽  
...  

2011 ◽  
Vol 74 (9) ◽  
pp. 1558-1563 ◽  
Author(s):  
J. E. LINE ◽  
N. J. STERN ◽  
B. B. OAKLEY ◽  
B. S. SEAL

An instrument (TEMPO) has been developed to automate the most-probable-number (MPN) technique and reduce the effort required to estimate some bacterial populations. We compared the automated MPN technique with traditional microbiological plating methods and Petrifilm methods for estimating the total viable count of aerobic microorganisms (TVC), total coliforms (CC), and Escherichia coli populations (EC) on freshly processed broiler chicken carcasses (postchill whole carcass rinse [WCR] samples) and cumulative drip-line samples from a commercial broiler processing facility. Overall, 120 broiler carcasses, 36 prechill drip-line samples, and 40 postchill drip-line samples were collected over 5 days (representing five individual flocks) and analyzed by the automated MPN and direct agar plating and Petrifilm methods. The TVC correlation coefficient between the automated MPN and traditional methods was very high (0.972) for the prechill drip samples, which had mean log-transformed values of 3.09 and 3.02, respectively. The TVC correlation coefficient was lower (0.710) for the postchill WCR samples, which had lower mean log values of 1.53 and 1.31, respectively. Correlations between the methods for the prechill CC and EC samples were 0.812 and 0.880, respectively. The estimated number of total aerobes was generally greater than the total number of coliforms or E. coli recovered for all sample types (P < 2e−16). Significantly more bacteria were recovered from the prechill samples than from the postchill WCR or cumulative drip samples (P < 9.5e−12 and P < 2e−16, respectively). When samples below the limit of detection were excluded, 92.1% of the total responses were within a single log difference between the traditional plating or Petrifilm methods and the automated MPN method.


2021 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Norhanizam Nordin ◽  
Nani Sani ◽  
Arifah Kadir ◽  
Rumaizi Shaari ◽  
Maizan Mohamed ◽  
...  

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Reza Talebiyan ◽  
Mehdi Kheradmand ◽  
Faham Khamesipour ◽  
Mohammad Rabiee-Faradonbeh

Antimicrobial agents are used extremely in order to reduce the great losses caused byEscherichia coliinfections in poultry industry. In this study, 318 pathogenicEscherichia coli(APEC) strains isolated from commercial broiler flocks with coli-septicemia were examined for antimicrobials of both veterinary and human significance by disc diffusion method. Multiple resistances to antimicrobial agents were observed in all the isolates. Resistance to the antibiotics was as follows: Tylosin (88.68%), Erythromycin (71.70%), Oxytetracycline (43.40%), Sulfadimethoxine-Trimethoprim (39.62%), Enrofloxacin (37.74%), Florfenicol (35.85%), Chlortetracycline (33.96%), Doxycycline (16.98%), Difloxacin (32.08%), Danofloxacin (28.30%), Chloramphenicol (20.75%), Ciprofloxacin (7.55%), and Gentamicin (5.66%). This study showed resistance against the antimicrobial agents that are commonly applied in poultry, although resistance against the antibiotics that are only applied in humans or less frequently used in poultry was significantly low. This study emphasizes on the occurrence of multiple drug resistantE. coliamong diseased broiler chickens in Iran. The data revealed the relative risks of using antimicrobials in poultry industry. It also concluded that use of antibiotics must be limited in poultry farms in order to reduce the antibiotic resistances.


2020 ◽  
Author(s):  
N. Simon Ekesi ◽  
Beata Dolka ◽  
Adnan A.K. Alrubaye ◽  
Douglas D. Rhoads

AbstractWe investigated lameness outbreaks at commercial broiler farms in Arkansas. From Bacterial Chondronecrosis with Osteomyelitis (BCO) lesions, we obtained different isolates of distinct bacterial species. Genome assemblies forEscherichia coliandStaphylococcus aureusisolates show that BCO-lameness pathogens on farms can differ significantly. Genomes assembled fromEscherichia coliisolates from three different farms were quite different from each other, and more similar to isolates from different hosts and geographical locations. The S aureus genomes were closely related to chicken isolates from Europe, and appear to have been restricted to chicken hosts for more than 40 years. Detailed analyses of genomes from this clade of chicken isolates with a sister clade of human isolates, suggests the acquisition of a particular pathogenicity island in the transition from human to chicken pathogen and that pathogenesis in chickens may depend on this mobile element. Phylogenomics is consistent with more frequent host shifts forE. coli, whileS. aureusappears to be highly host restricted. Isolate-specific genome characterizations will help further our understanding of the disease mechanisms and spread of BCO-lameness, a significant animal welfare issue.ImportanceDetailed inspection of the genome sequences of different bacterial species associated with causing lameness in broiler chickens reveals that one species,E. coli, appears to easily switch hosts from humans to chickens and other host species. Conversely, isolates ofS. aureusappear to be restricted to specific hosts. One potential mobile DNA element has been identified that may be critical for causing disease in chickens forS. aureus.


2016 ◽  
Vol 95 (5) ◽  
pp. 1105-1115 ◽  
Author(s):  
H.N. Chinivasagam ◽  
W. Estella ◽  
H. Rodrigues ◽  
D.G. Mayer ◽  
C. Weyand ◽  
...  

2006 ◽  
Vol 148 (6) ◽  
pp. 289-295 ◽  
Author(s):  
C. Zweifel ◽  
M. Kaufmann ◽  
J. Blanco ◽  
R. Stephan

2018 ◽  
Vol 5 (4) ◽  
Author(s):  
Mohamed M Amer ◽  
Hagar M Ahmed ◽  
Khaled M Elbayoumi ◽  
Mohamed A Kutkat

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