Exogenous carbon source and phytohormone supplementation enhanced growth rate and metabolite production in freshwater microalgae Scenedesmus obtusus Meyen

2021 ◽  
pp. 100669
Author(s):  
Aravind K. Vijay ◽  
Said Ali M. Salim ◽  
Syama Prabha ◽  
Basil George
Keyword(s):  
Growth Rate ◽  
Carbon Source ◽  
Freshwater Microalgae ◽  
Metabolite Production ◽  
Exogenous Carbon Source

BIOSYNTHESIS OF THE POLYPHENOLIC ACID METABOLITES OF POLYPORUS TUMULOSUS COOKE

1967 ◽  
Vol 13 (2) ◽  
pp. 181-197 ◽  
Author(s):  
R. K. Crowden
Keyword(s):  
Growth Rate ◽  
Carbon Source ◽  
Shikimic Acid ◽  
High Growth ◽  
High Growth Rate ◽  
Fungal Metabolite ◽  
Gentisic Acid ◽  
Metabolite Production ◽  
Acid Metabolites ◽  
Acid Pathway

Growth and metabolite production by Polyporus tumulosus Cooke were shown to be markedly influenced by (a) the nature of the carbon source, (b) the carbon mitrogen ratio, and (c) the pH of the medium at the time of inoculation. Whereas in some instances growth and metabolite production could be altered independently, production of phenols was more usually correlated with a high growth rate. Further, variations in the conditions of culture were shown to alter the relative proportions of the several phenolic acids as well as the gross yield.The biochemical interrelationships of the metabolites were also investigated. Isotope incorporation experiments demonstrated that these compounds have their biosynthetic origin in the shikimic acid pathway rather than by that of acetate condensation. The primary shunt metabolites were located on two similar pathways and a probable relationship for the secondary shunt metabolites is suggested. The investigation has shown that the well-known fungal metabolite gentisic acid is produced in a biochemical sequence alternative to that usually described for this compound in microorganisms.

Biophotolysis-based hydrogen and lipid production by oleaginous microalgae using crude glycerol as exogenous carbon source

2017 ◽  
Vol 42 (4) ◽  
pp. 1970-1976 ◽  
Author(s):  
Dennapa Sengmee ◽  
Benjamas Cheirsilp ◽  
Thanwadee Tachapattaweawrakul Suksaroge ◽  
Poonsuk Prasertsan
Keyword(s):  
Carbon Source ◽  
Crude Glycerol ◽  
Lipid Production ◽  
Oleaginous Microalgae ◽  
Exogenous Carbon Source

Production of metabolites from chlorobiphenyls by resting cells ofPseudomonasstrain LB400 after growth on different carbon sources

1999 ◽  
Vol 45 (2) ◽  
pp. 178-184 ◽  
Author(s):  
K A Billingsley ◽  
S M Backus ◽  
O P Ward
Keyword(s):  
Carbon Source ◽  
Polychlorinated Biphenyl ◽  
Carbon Sources ◽  
Resting Cells ◽  
Metabolite Formation ◽  
Chlorobenzoic Acid ◽  
Metabolite Production ◽  
Pcb Congeners ◽  
Chlorobenzoic Acids ◽  
General Order

Cells of Pseudomonas strain LB400, grown on biphenyl, glucose, or glycerol, transformed polychlorinated biphenyl (PCB) congeners into chlorobenzoic acid (CBA) metabolites. Transformation of the PCB congeners, 2,3-chlorobiphenyl (CBP), 2,2'-CBP, 2,5,4'-CBP, and 2,4,2',4'-CBP, produced the metabolites, 2,3-CBA, 2-CBA, 4-CBA, and 2,4-CBA, respectively. Rates and extents of PCB transformation and metabolite formation were highest with biphenyl-grown cells. Intermediate rates of metabolite production were observed with glycerol-grown cells, and lowest rates of production were found with glucose-grown cells. Regardless of carbon source, the rate of degradation of congeners was faster than the rate of production of CBAs. Relative rates of PCB transformation and metabolite production from different congeners with cells grown on a particular substrate followed the same general order, 2,3-CBA (from 2,3-CBP) > 2-CBA (from 2,2'-CBP) > 4-CBA (from 2,5,4'-CBP) > 2,4-CBA (from 2,4,2',4'-CBP). Pseudomonas strain LB400 appeared unable to grow on any of the chlorobenzoic acids. However, Pseudomonas strain LB400 cells grown on biphenyl appeared capable of degrading 2-CBA and 2,3-CBA but not 4-CBA nor 2,4-CBA. Cells grown on glycerol appeared unable to metabolize any CBAs.Key words: polychlorinated biphenyls, metabolites, Pseudomonas LB400.

A comparison between ethanol and methanol as carbon sources for denitrification

1994 ◽  
Vol 30 (6) ◽  
pp. 83-90 ◽  
Author(s):  
Magnus Christensson ◽  
Ewa Lie ◽  
Thomas Welander
Keyword(s):  
Growth Rate ◽  
Carbon Source ◽  
Pure Culture ◽  
Carbon Sources ◽  
Culture Studies ◽  
Adaptation Time ◽  
Synthetic Media ◽  
Short Time

Ethanol and methanol were compared for their performance as carbon sources for denitrification. The study was carried out in two chemostats, operated in parallel on synthetic media containing ethanol and methanol respectively as carbon sources. In addition, pure culture studies were performed on one ethanol- and one methanol-utilizing denitrifier. Ethanol was found to be considerably more readily available as a carbon source for denitrification than was methanol. An efficient denitrification with ethanol was established in a short time, while denitrification with methanol required a substantial adaptation time and never showed the same stability as denitrification with ethanol. The growth rate of denitrifiers with ethanol as carbon source was 2–3 times higher than with methanol. The amount of COD required to denitrify a certain amount of nitrate was somewhat lower for ethanol (3.85 g/gN) than for methanol (4.45 g/gN) in the continuous experiments, while it was considerably higher for ethanol (6.1 g/gN) than for methanol (4.1 g/gN) in pure culture batch cultivations.

Nutritional requirements for chloramphenicol biosynthesis in Streptomyces venezuelae

1983 ◽  
Vol 29 (2) ◽  
pp. 247-253 ◽  
Author(s):  
S. Chatterjee ◽  
L. C. Vining ◽  
D. W. S. Westlake
Keyword(s):  
Growth Rate ◽  
Carbon Source ◽  
Mineral Nutrition ◽  
Phase Variation ◽  
Nutritional Requirements ◽  
Carbon Source Utilization ◽  
Streptomyces Venezuelae ◽  
Lactate Medium ◽  
Inoculation Procedure ◽  
Medium Preparation

Cultures grown in a glycerol serine lactate medium were used to establish the inoculation procedure, aeration level, and trace-mineral nutrition optimizing chloramphenicol production in Streptomyces venezuelae. The stimulatory effect of lactate in this medium was concluded not to be an artifact of medium preparation but to reside in its influence on carbon-source utilization. In media with ammonium sulfate as a nonrestricting source of nitrogen, chloramphenicol production varied with the carbon source chosen. Production occurred during the growth phase and was highest on galactose, lactose, cellobiose, and starch. The rate of synthesis was related directed to the growth rate and decreased in the stationary phase. Variation of the nitrogen source with glucose as a nonrestricting source of carbon showed that the highest antibiotic titres were obtained with poorly utilized compounds such as isoleucine or phenylalanine. Proline gave yields comparable with those obtained in the more complex glycerol serine lactate medium in a shorter time. Although rate of growth is not the sole determining parameter, chloramphenicol synthesis is concluded to be a "growth-linked" process.

scholarly journals Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast

2011 ◽  
Vol 22 (12) ◽  
pp. 1997-2009 ◽  
Author(s):  
Nikolai Slavov ◽  
David Botstein
Keyword(s):  
Growth Rate ◽  
Oxygen Consumption ◽  
Cell Division ◽  
Carbon Source ◽  
Oxidative Metabolism ◽  
Sole Source ◽  
Cell Division Cycle ◽  
Steady State Responses ◽  
Yeast Metabolic Cycle ◽  
Metabolic Cycle

We studied the steady-state responses to changes in growth rate of yeast when ethanol is the sole source of carbon and energy. Analysis of these data, together with data from studies where glucose was the carbon source, allowed us to distinguish a “universal” growth rate response (GRR) common to all media studied from a GRR specific to the carbon source. Genes with positive universal GRR include ribosomal, translation, and mitochondrial genes, and those with negative GRR include autophagy, vacuolar, and stress response genes. The carbon source–specific GRR genes control mitochondrial function, peroxisomes, and synthesis of vitamins and cofactors, suggesting this response may reflect the intensity of oxidative metabolism. All genes with universal GRR, which comprise 25% of the genome, are expressed periodically in the yeast metabolic cycle (YMC). We propose that the universal GRR may be accounted for by changes in the relative durations of the YMC phases. This idea is supported by oxygen consumption data from metabolically synchronized cultures with doubling times ranging from 5 to 14 h. We found that the high oxygen consumption phase of the YMC can coincide exactly with the S phase of the cell division cycle, suggesting that oxidative metabolism and DNA replication are not incompatible.

scholarly journals The Growth Comparison of Haematococcus Pluvialis in Two Different Medium

Biota ◽  
2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Dina Soes Putri ◽  
Siti Alaa
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Haematococcus Pluvialis ◽  
Marine Ecosystem ◽  
Culture Conditions ◽  
Vital Role ◽  
Freshwater Microalgae ◽  
Inoculum Concentration ◽  
Chemical Factors ◽  
Physical And Chemical

Microalgae is an aquatic microorganism that conducts photosynthesis. It plays a vital role as an oxygen producer in the marine ecosystem. A freshwater microalgae, Haematococcus pluvialis, has been utilized as a health supplement and industrial application which is beneficial for human. In addition to physical and chemical factors, nutrient composition is one crucial thing that contributes to the growth of microalgae. This present study aimed to determine and compare the growth rate of Haematococcus pluvialis cultivated in two culture medium, Walne’s and Guillard. The culture conditions observed were light intensity, photoperiod of light-dark hours, temperature, inoculum concentration of medium’s liquid, and cell density. This study confirmed that Walne’s media produced much higher biomass (247x104 cells/mL) than Guillard’s medium (209.6x104 cells/mL). The aspect to be further performed on  H. pluvialis biomass is exploring its high-value bio compound. 

scholarly journals Mapping the Fundamental Niches of Two Freshwater Microalgae,Chlorella vulgaris(Trebouxiophyceae) andPeridinium cinctum(Dinophyceae), in 5-Dimensional Ion Space

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Terence J. Evens ◽  
Randall P. Niedz
Keyword(s):  
Growth Rate ◽  
Experimental Design ◽  
Chlorella Vulgaris ◽  
Growth Rates ◽  
Ion Concentration ◽  
Freshwater Microalgae ◽  
Batch Cultures ◽  
Fundamental Niche ◽  
Maximum Cell ◽  
Cell Densities

The fundamental niche defined by five ions,NO3 −,PO4 3−, K+, Na+, andCl−, was mapped forChlorella vulgaris(Trebouxiophyceae) andPeridinium cinctum(Dinophyceae) growth rates and maximum cell densities in batch cultures. A five dimensional ion-mixture experimental design was projected across a total ion concentration gradient of 1 to 30 mM to delineate the ion-based, “potential” niche space, defined as the entiren-dimensional hypervolume demarcated by the feasible ranges of the independent factors under consideration. The growth rate-based, fundamental niche volumes overlapped for ca. 94% of the ion mixtures, although the regions of maximal growth rates and cell densities were different for each alga. BothC. vulgarisandP. cinctumexhibited similar positive responses to cations and negative responses to anions. It was determined that total ion concentration for these five ions, from 1 to 30 mM, did not directly affect either growth rate or maximal cell density for either alga, although it did play an interactive role with several ions. This study is the first that we are aware of to attempt the mapping of a multivariate, ion-based, fundamental niche volume. The implications of the experimental design utilized and the potential utility of this type of approach are discussed.

Assessment of different carbohydrates as exogenous carbon source in cultivation of cyanobacteria

2014 ◽  
Vol 37 (8) ◽  
pp. 1497-1505 ◽  
Author(s):  
Érika Cristina Francisco ◽  
Telma Teixeira Franco ◽  
Roger Wagner ◽  
Eduardo Jacob-Lopes
Keyword(s):  
Carbon Source ◽  
Exogenous Carbon Source
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