scholarly journals Retraction notice to “A SERS-based lateral flow assay biosensor for quantitative and ultrasensitive detection of interleukin-6 in unprocessed whole blood” [Biosens. Bioelectron. 141 (2019) 111432]

2020 ◽  
Vol 168 ◽  
pp. 112415
Author(s):  
Ying Wang ◽  
Jingyi Sun ◽  
Yajun Hou ◽  
Cheng Zhang ◽  
Dawei Li ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Whole Blood ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Ultrasensitive Detection ◽  
Retraction Notice

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM).The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated at a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM). The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

A lateral flow assay for the determination of human tetanus antibody in whole blood by using gold nanoparticle labeled tetanus antigen

2018 ◽  
Vol 185 (2) ◽  
Author(s):  
Juanzu Liu ◽  
Junyan Wang ◽  
Zhaohui Li ◽  
Hongmin Meng ◽  
Lin Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Whole Blood ◽  
Lateral Flow ◽  
Lateral Flow Assay

scholarly journals Ultrasensitive Detection of Severe Fever with Thrombocytopenia Syndrome Virus Based on Immunofluorescent Carbon Dots/SiO2 Nanosphere-Based Lateral Flow Assay

ACS Omega ◽  
2019 ◽  
Vol 4 (25) ◽  
pp. 21431-21438 ◽  
Author(s):  
Lai-Di Xu ◽  
Qing Zhang ◽  
Shou-Nian Ding ◽  
Jing-Juan Xu ◽  
Hong-Yuan Chen
Keyword(s):  
Carbon Dots ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Ultrasensitive Detection ◽  
Severe Fever

Lateral Flow Assay for Interleukin 6: A Technological and Scientific Prospection of a 10-Year Survey

2018 ◽  
Vol 12 ◽  
Author(s):  
Ingridi Souza Sene ◽  
Vladimir Costa Silva ◽  
Gisele Elias Nunes Pauli ◽  
Ivan Helmuth Bechtold ◽  
Livio Cesar Cunha Nunes ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Lateral Flow ◽  
Lateral Flow Assay

scholarly journals 1791Evaluating Cryptococcal Antigen Lateral Flow Assay by Fingerstick Whole Blood in HIV-Infected Persons with Meningitis

2014 ◽  
Vol 1 (suppl_1) ◽  
pp. S60-S60 ◽  
Author(s):  
Darlisha Williams ◽  
Kiiza Tadeo ◽  
Sruti Velamakkani ◽  
Reuben Kiggundu ◽  
David Meya ◽  
...  
Keyword(s):  
Whole Blood ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Cryptococcal Antigen

Correcting the effect of hematocrit in whole blood coagulation analysis on paper-based lateral flow device

2018 ◽  
Vol 10 (24) ◽  
pp. 2869-2874 ◽  
Author(s):  
Hua Li ◽  
Daewoo Han ◽  
Giovanni M. Pauletti ◽  
Michael A. Hegener ◽  
Andrew J. Steckl
Keyword(s):  
Blood Coagulation ◽  
Whole Blood ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Simple Method ◽  
Lateral Flow Device ◽  
Flow Device

A simple method to correct the effect of hematocrit (Hct) on a paper-based lateral flow assay device that is designed to assess whole blood coagulation is reported.

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