FRET probe-based antibacterial susceptibility testing (F-AST) by detection of bacterial nucleases released by antibiotic-induced lysis

2019 ◽  
Vol 130 ◽  
pp. 225-229 ◽  
Author(s):  
Kyoungsook Park ◽  
Jinyoung Jeong ◽  
So Yeon Yi ◽  
Wang Sik Lee ◽  
Yong Beom Shin
Keyword(s):  
Susceptibility Testing ◽  
Antibacterial Susceptibility

Quicolor: A novel system for rapid antibacterial susceptibility testing

2007 ◽  
Vol 57 (1) ◽  
pp. 131-135 ◽  
Author(s):  
Tanil Kocagöz ◽  
Serpil Ercis ◽  
Özge Darka ◽  
Siavosh Salmanzadeh-Ahrabi ◽  
Sesin Kocagöz ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antibacterial Susceptibility

scholarly journals In Vitro Activity of Iclaprim against Isolates in Two Phase 3 Clinical Trials (REVIVE-1 and -2) for Acute Bacterial Skin and Skin Structure Infections

2019 ◽  
Vol 63 (4) ◽  
Author(s):  
Stephanie Noviello ◽  
Sophie Magnet ◽  
Stephen Hawser ◽  
David B. Huang
Keyword(s):  
Susceptibility Testing ◽  
In Vitro Activity ◽  
Phase 3 ◽  
Gram Positive ◽  
Two Phase ◽  
Content Type ◽  
Skin Structure ◽  
Streptococcus Anginosus ◽  
Antibacterial Susceptibility

ABSTRACT Iclaprim, a selective bacterial dihydrofolate reductase inhibitor, and other antibiotics were tested against Gram-positive isolates from two phase 3 studies of acute bacterial skin and skin structure infections (ABSSSIs) (REVIVE-1 and -2). Seven hundred ninety baseline isolates, including Staphylococcus aureus, β-hemolytic streptococci, and Streptococcus anginosus group, underwent antibacterial susceptibility testing. Iclaprim had an MIC90 of 0.12 μg/ml for S. aureus (0.12 μg/ml for methicillin susceptible, 0.25 μg/ml for methicillin resistant), 0.25 μg/ml for β-hemolytic streptococci, and 0.008 μg/ml for S. anginosus group. Iclaprim demonstrated potent activity against these Gram-positive ABSSSI isolates.

scholarly journals Antifungal Susceptibility Testing: Current Approaches

2020 ◽  
Vol 33 (3) ◽  
Author(s):  
Elizabeth L. Berkow ◽  
Shawn R. Lockhart ◽  
Luis Ostrosky-Zeichner
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Epidemiological Studies ◽  
Antifungal Susceptibility Testing ◽  
Antifungal Drug Resistance ◽  
Development Organizations ◽  
Gradient Diffusion ◽  
Antibacterial Susceptibility ◽  
Automated Instruments

SUMMARY Although not as ubiquitous as antibacterial susceptibility testing, antifungal susceptibility testing (AFST) is a tool of increasing importance in clinical microbiology laboratories. The goal of AFST is to reliably produce MIC values that may be used to guide patient therapy, inform epidemiological studies, and track rates of antifungal drug resistance. There are three methods that have been standardized by standards development organizations: broth dilution, disk diffusion, and azole agar screening for Aspergillus. Other commonly used methods include gradient diffusion and the use of rapid automated instruments. Novel methodologies for susceptibility testing are in development. It is important for laboratories to consider not only the method of testing but also the interpretation (or lack thereof) of in vitro data.

Antibacterial Susceptibility Patterns and Characterization of Clinical Isolates of Elizabethkingia meningoseptica in Henan Province, China

2013 ◽  
Vol 295-298 ◽  
pp. 560-563
Author(s):  
Tao Yu ◽  
Hao Ying ◽  
Zhen Bin Wu
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Hospital Environment ◽  
Practical Significance ◽  
Elizabethkingia Meningoseptica ◽  
Environmental Surfaces ◽  
Antibacterial Susceptibility ◽  
Insight Into

This study aimed to provide insight into antimicrobial susceptibility and homology of Elizabethkingia meningoseptica in a hospital environment. Samples from environmental surfaces and the hands of medical staff were screened for E. meningoseptica and antimicrobial susceptibility testing was performed; Pulsed-Field Gel Electrophoresis (PFGE) was employed to subtype E. meningoseptica strains; The resistant genes were detected by PCR. In total, six isolates of E. meningoseptica were collected from 280 samples. Antimicrobial susceptibility testing revealed that all of the six strains displayed multiresistance, showing resistance to more than three different classes of antibiotics. The strains were separated into five different PFGE patterns. The sulII gene was detected in four of the strains. Our data show that multiresistant E. meningoseptica strains exist in the hospital environment and susceptibility testing revealed that vancomycin was the most effective antibiotic. These results have practical significance for treatment of E. meningoseptica infection.

P1648 A new mass-spectrometric approach to antibacterial susceptibility testing

2007 ◽  
Vol 29 ◽  
pp. S465
Author(s):  
V. Vereshchagin ◽  
E. Ilina ◽  
M. Atroshkina ◽  
M. Serebryakova ◽  
T. Maier ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Mass Spectrometric ◽  
Mass Spectrometric Approach ◽  
Antibacterial Susceptibility

scholarly journals Antibacterial activity of aquatic extract of Myrtus communis leaves against Periodontitis isolated bacteria

2021 ◽  
Vol 880 (1) ◽  
pp. 012047
Author(s):  
Eman Mubdir Nayf ◽  
Hamzah Abdulrhaman Salman
Keyword(s):  
Susceptibility Testing ◽  
Morphological Characterization ◽  
Diffusion Method ◽  
Potential Zone ◽  
Myrtus Communis ◽  
Zone Of Inhibition ◽  
Biochemical Tests ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Antibacterial Susceptibility

Abstract Myrtus communis is an evergreen plant that can survive stressful environments and high-temperature seasons. Treatment using green plants was the most trended in recent years. The present study aimed to evaluate the antibacterial effects of Myrtus communis leaves against bacteria isolated from periodontitis. Fifty samples were collected from periodontitis subjects in both genders (female 32 % and male 68 %). The isolates were diagnosed by morphological characterization and biochemical tests. M. communis leaves were identified, collected, and prepared for extraction. The plant leaves were extracted using distilled water. The antibacterial susceptibility testing was performed by the well diffusion method. Antibiotics susceptibility patterns were executed using the disc diffusion method. All the isolates belonged to gram-positive bacteria. Among the isolated bacteria, 20, 18, and 12 were Lactobacillus spp., Streptococcus spp., and Staphylococcus aureus, respectively. The antibacterial susceptibility testing of M. communis extract showed a potential zone of inhibition against all the tested bacteria. Of the different concentrations, 30 mg/ml showed the highest zone of inhibition, 18.2 mm, 19.50 mm, and 30.66 mm against Streptococcus spp., Staphylococcus spp., and Lactobacillus spp. Among the tested antibiotics, ciprofloxacin and chloramphenicol exhibited the highest zone of inhibition against the tested bacteria. The aquatic extract of M. communis leaves was found to be effective against gram-positive bacteria. Further studies are warranted to investigate the active bio-compounds.

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