Staphylococcus aureus Specific FRET Probe-Based Antibacterial Susceptibility Testing (SF-AST) by Detection of Micrococcal Nuclease Activity

2019 ◽  
Vol 6 (2) ◽  
pp. 215-223
Author(s):  
So Yeon Yi ◽  
Jinyoung Jeong ◽  
Kyoon Eon Kim ◽  
Kyoungsook Park ◽  
Yong Beom Shin
Keyword(s):  
Staphylococcus Aureus ◽  
Susceptibility Testing ◽  
Nuclease Activity ◽  
Micrococcal Nuclease ◽  
Antibacterial Susceptibility

Ultrasensitive detection of micrococcal nuclease activity and Staphylococcus aureus contamination using optical biosensor technology-A review

Talanta ◽  
2021 ◽  
Vol 226 ◽  
pp. 122168
Author(s):  
Somayeh Sahraneshin Samani ◽  
Amir Khojastehnezhad ◽  
Mohammad Ramezani ◽  
Mona Alibolandi ◽  
Farideh Tabatabaei Yazdi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Nuclease Activity ◽  
Optical Biosensor ◽  
Micrococcal Nuclease ◽  
Ultrasensitive Detection ◽  
Biosensor Technology ◽  
And Staphylococcus Aureus

scholarly journals The Frequency of Methicillin-Resistant Staphylococcus aureus and Coagulase Gene Polymorphism in Egypt

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hend M. Abdulghany ◽  
Rasha M. Khairy
Keyword(s):  
Staphylococcus Aureus ◽  
Gene Polymorphism ◽  
Antimicrobial Susceptibility ◽  
Antimicrobial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Methicillin Resistant ◽  
Microbiological Methods ◽  
Rflp Typing

The current study aimed to use Coagulase gene polymorphism to identify methicillin-resistant Staphylococcus aureus (MRSA) subtypes isolated from nasal carriers in Minia governorate, Egypt, evaluate the efficiency of these methods in discriminating variable strains, and compare these subtypes with antibiotypes. A total of 400 specimens were collected from nasal carriers in Minia governorate, Egypt, between March 2012 and April 2013. Fifty-eight strains (14.5%) were isolated and identified by standard microbiological methods as MRSA. The identified isolates were tested by Coagulase gene RFLP typing. Out of 58 MRSA isolates 15 coa types were classified, and the amplification products showed multiple bands (1, 2, 3, 4, 5, and 8 bands). Coagulase gene PCR-RFLPs exhibited 10 patterns that ranged from 1 to 8 fragments with AluI digestion. Antimicrobial susceptibility testing with a panel of 8 antimicrobial agents showed 6 different antibiotypes. Antibiotype 1 was the most common phenotype with 82.7%. The results have demonstrated that many new variants of the coa gene are present in Minia, Egypt, different from those reported in the previous studies. So surveillance of MRSA should be continued.

scholarly journals Evaluation of Staphylococcal Activity of Garcinia kola Almonds

2019 ◽  
pp. 1-7
Author(s):  
Ouattara Karamoko ◽  
Dibi Koffi Saint Didier ◽  
Kone Monon ◽  
Ouattara Abou ◽  
Bagre Issa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Reference Strain ◽  
Susceptibility Testing ◽  
Traditional Society ◽  
In Vitro Growth ◽  
Liquid Media ◽  
Cost Of Treatment ◽  
Garcinia Kola

The emergence of infectious diseases, particularly staphylococcal infections, treatment failures and the more high cost of treatment of infections caused by resistant staphylococci called to find other care alternatives. This study was initiated to evaluate the antibacterial activity of the aqueous extract from Garcinia kola almonds on the in vitro growth of Staphylococcus aureus strains. The methods of diffusion in agar and liquid media were used for susceptibility testing and MIC and MBC determination. The tests were performed on four strains of S. aureus and one reference strain. The minimum inhibitory concentrations of the extracts ranged from 3.12 mg/mL and 12.5 mg/mL and the minimum bactericidal concentrations between 6.25 mg/mL and 25 mg/mL. The lowest value of MIC and MBC was observed with S. aureus ATCC 29213 while the greatest value of these same parameters was obtained on S. aureus 993C/18 and S. aureus 1075C/18. The aqueous almonds extract of Garcinia kola had a bactericidal activity on all the strains of S. aureus studied. This could justify the use of Garcinia kola almonds in the treatment of various diseases in traditional society.

Detection of micrococcal nuclease for identifying Staphylococcus aureus based on DNA templated fluorescent copper nanoclusters

2019 ◽  
Vol 186 (4) ◽  
Author(s):  
Taiping Qing ◽  
Caicheng Long ◽  
Xuan Wang ◽  
Kaiwu Zhang ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Micrococcal Nuclease ◽  
Copper Nanoclusters

scholarly journals Summary of Linezolid Activity and Resistance Mechanisms Detected during the 2012 LEADER Surveillance Program for the United States

2013 ◽  
Vol 58 (2) ◽  
pp. 1243-1247 ◽  
Author(s):  
Rodrigo E. Mendes ◽  
Robert K. Flamm ◽  
Patricia A. Hogan ◽  
James E. Ross ◽  
Ronald N. Jones
Keyword(s):  
United States ◽  
Staphylococcus Aureus ◽  
Susceptibility Testing ◽  
The United States ◽  
Resistance Mechanisms ◽  
Regulatory Approval ◽  
Surveillance Program ◽  
Gram Positive ◽  
Content Type

ABSTRACTThis study summarizes the linezolid susceptibility testing results for 7,429 Gram-positive pathogens from 60 U.S. sites collected during the 2012 sampling year for the LEADER Program. Linezolid showed potent activity when tested against 2,980Staphylococcus aureusisolates, inhibiting all but 3 at ≤2 μg/ml. Similarly, linezolid showed coverage against 99.5% of enterococci, as well as for all streptococci tested. These results confirm a long record of linezolid activity against U.S. Gram-positive isolates since regulatory approval in 2000.

Quicolor: A novel system for rapid antibacterial susceptibility testing

2007 ◽  
Vol 57 (1) ◽  
pp. 131-135 ◽  
Author(s):  
Tanil Kocagöz ◽  
Serpil Ercis ◽  
Özge Darka ◽  
Siavosh Salmanzadeh-Ahrabi ◽  
Sesin Kocagöz ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antibacterial Susceptibility
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