A fluorescence biosensor for VEGF detection based on DNA assembly structure switching and isothermal amplification

2017 ◽  
Vol 89 ◽  
pp. 964-969 ◽  
Author(s):  
Junlong Li ◽  
Kexin Sun ◽  
Zhongping Chen ◽  
Jifei Shi ◽  
Dandan Zhou ◽  
...  
Keyword(s):  
Isothermal Amplification ◽  
Dna Assembly ◽  
Assembly Structure ◽  
Fluorescence Biosensor ◽  
Structure Switching

Selective and rapid detection of mercury ion based on DNA assembly and nicking endonuclease-assisted signal amplification

2019 ◽  
Vol 11 (24) ◽  
pp. 3073-3078 ◽  
Author(s):  
Junling Liu ◽  
Liping Liu ◽  
Jun Chen ◽  
Tianchen Wang ◽  
Yuzhi Xu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rapid Detection ◽  
Mercury Ion ◽  
Isothermal Amplification ◽  
Dna Assembly ◽  
Nicking Endonuclease ◽  
Fast Detection ◽  
Assembly Structure ◽  
Amplification Strategy

An I-shaped DNA assembly structure coupled with endonuclease-assisted isothermal amplification strategy was constructed for sensitive and fast detection of Hg(ii).

Target-induced structure switching of a hairpin aptamer for the fluorescence detection of zeatin

2016 ◽  
Vol 8 (30) ◽  
pp. 5957-5961 ◽  
Author(s):  
Chen Liu ◽  
Jinyang Chen ◽  
Guobin Mao ◽  
Chen Su ◽  
Xinghu Ji ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Fluorescence Biosensor ◽  
Structure Switching ◽  
Induced Structure

A versatile, simple and sensitive fluorescence biosensor is developed for zeatin detection based on target-induced structure switching of hairpin aptamer.

Biosensing by Tandem Reactions of Structure Switching, Nucleolytic Digestion, and DNA Amplification of a DNA Assembly

2015 ◽  
Vol 54 (33) ◽  
pp. 9637-9641 ◽  
Author(s):  
Meng Liu ◽  
Wenqing Zhang ◽  
Qiang Zhang ◽  
John D. Brennan ◽  
Yingfu Li
Keyword(s):  
Dna Amplification ◽  
Dna Assembly ◽  
Tandem Reactions ◽  
Structure Switching

scholarly journals Producing molecular biology reagents without purification

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252507
Author(s):  
Sanchita Bhadra ◽  
Vylan Nguyen ◽  
Jose-Angel Torres ◽  
Shaunak Kar ◽  
Stéphane Fadanka ◽  
...  
Keyword(s):  
United Kingdom ◽  
Synthetic Biology ◽  
Molecular Biology ◽  
Reverse Transcription ◽  
Isothermal Amplification ◽  
Dna Assembly ◽  
Golden Gate ◽  
Local Production ◽  
The United Kingdom ◽  
Simplified Method

We recently developed ‘cellular’ reagents–lyophilized bacteria overexpressing proteins of interest–that can replace commercial pure enzymes in typical diagnostic and molecular biology reactions. To make cellular reagent technology widely accessible and amenable to local production with minimal instrumentation, we now report a significantly simplified method for preparing cellular reagents that requires only a common bacterial incubator to grow and subsequently dry enzyme-expressing bacteria at 37°C with the aid of inexpensive chemical desiccants. We demonstrate application of such dried cellular reagents in common molecular and synthetic biology processes, such as PCR, qPCR, reverse transcription, isothermal amplification, and Golden Gate DNA assembly, in building easy-to-use testing kits, and in rapid reagent production for meeting extraordinary diagnostic demands such as those being faced in the ongoing SARS-CoV-2 pandemic. Furthermore, we demonstrate feasibility of local production by successfully implementing this minimized procedure and preparing cellular reagents in several countries, including the United Kingdom, Cameroon, and Ghana. Our results demonstrate possibilities for readily scalable local and distributed reagent production, and further instantiate the opportunities available via synthetic biology in general.

Biosensing by Tandem Reactions of Structure Switching, Nucleolytic Digestion, and DNA Amplification of a DNA Assembly

2015 ◽  
Vol 127 (33) ◽  
pp. 9773-9777 ◽  
Author(s):  
Meng Liu ◽  
Wenqing Zhang ◽  
Qiang Zhang ◽  
John D. Brennan ◽  
Yingfu Li
Keyword(s):  
Dna Amplification ◽  
Dna Assembly ◽  
Tandem Reactions ◽  
Structure Switching

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

The influence of SYBR Green I on isothermal amplification with Bst exo DNA polymerase.

Biomics ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 268-273
Author(s):  
A.R. Gilvanov ◽  
A.R. Sakhabutdinova ◽  
A.V. Chemeris ◽  
R.R. Garafutdinov
Keyword(s):  
Dna Polymerase ◽  
Sybr Green ◽  
Isothermal Amplification ◽  
Sybr Green I
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