Metal-enhanced fluorescent dye-doped silica nanoparticles and magnetic separation: A sensitive platform for one-step fluorescence detection of prostate specific antigen

2017 ◽  
Vol 87 ◽  
pp. 881-887 ◽  
Author(s):  
Dang-Dang Xu ◽  
Yun-Liang Deng ◽  
Cheng-Yu Li ◽  
Yi Lin ◽  
Hong-Wu Tang
Keyword(s):  
Silica Nanoparticles ◽  
Prostate Specific Antigen ◽  
Magnetic Separation ◽  
Fluorescence Detection ◽  
Specific Antigen ◽  
Fluorescent Dye ◽  
One Step

Single chip SPR and fluorescent ELISA assay of prostate specific antigen

Lab on a Chip ◽  
2015 ◽  
Vol 15 (23) ◽  
pp. 4433-4440 ◽  
Author(s):  
J. Breault-Turcot ◽  
H.-P. Poirier-Richard ◽  
M. Couture ◽  
D. Pelechacz ◽  
J.-F. Masson
Keyword(s):  
Surface Plasmon Resonance ◽  
Prostate Specific Antigen ◽  
Surface Plasmon ◽  
Fluorescence Detection ◽  
Plasmon Resonance ◽  
Specific Antigen ◽  
Fluorescence Microscope ◽  
Elisa Assay ◽  
Single Chip ◽  
Channel System

A multi-channel system combining fluidics and micropatterned plasmonic materials with wavelength interrogation surface plasmon resonance (SPR) and fluorescence detection was integrated from the combination of a small and motorized fluorescence microscope mounted on a portable 4-channel SPR instrument.

One-step immunostrip test for the simultaneous detection of free and total prostate specific antigen in serum

2005 ◽  
Vol 307 (1-2) ◽  
pp. 1-12 ◽  
Author(s):  
César Fernández-Sánchez ◽  
Calum J. McNeil ◽  
Keith Rawson ◽  
Olle Nilsson ◽  
Hing Y. Leung ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Simultaneous Detection ◽  
Total Prostate Specific Antigen ◽  
One Step

Dual-label one-step immunoassay for simultaneous measurement of free and total prostate-specific antigen concentrations and ratios in serum

1995 ◽  
Vol 41 (8) ◽  
pp. 1115-1120 ◽  
Author(s):  
K Mitrunen ◽  
K Pettersson ◽  
T Piironen ◽  
T Björk ◽  
H Lilja ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Simultaneous Measurement ◽  
Solid Phase ◽  
Characteristic Curve ◽  
Specific Antigen ◽  
Sample Volume ◽  
Free Psa ◽  
One Step ◽  
Total Psa ◽  
Dual Label

Abstract We developed a simple one-step dual-label immunoassay for simultaneous measurement of the free, noncomplexed form of prostate-specific antigen (PSA) and total PSA. The assay is based on time-resolved fluorescence and includes a stable fluorescent chelate of Eu to label a monoclonal antibody (mAb) that detects only free PSA, whereas a second mAb labeled with a fluorescent chelate of Tb provides equimolar detection of both free PSA and PSA complexed to alpha 1-antichymotrypsin. A third mAb on a solid phase captures the free and complexed forms of PSA in an equimolar fashion. The simultaneous measurement of the free-to-total PSA ratio (F/T) with the one-step dual assay is not sensitive to variations in the sample volume. The discrimination between benign prostatic hyperplasia and prostate cancer patients, i.e., the area under the receiver-operating characteristic curve, increased from 0.64 (total PSA assay) to 0.78 and 0.81 when the F/T ratio was measured with single and dual assays, respectively.

Rapid one-step assay for prostate specific antigen in whole blood using an optical immunoassay technology

1995 ◽  
Author(s):  
Grenville Robinson ◽  
Timothy Bacarese-Hamilton ◽  
Paul M. O'Neill ◽  
Janys E. Fletcher ◽  
Phelim B. Daniels ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Whole Blood ◽  
Specific Antigen ◽  
One Step

scholarly journals Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 33
Author(s):  
Sungje Bock ◽  
Hyung-Mo Kim ◽  
Jaehi Kim ◽  
Jaehyun An ◽  
Yun-Sik Choi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Silica Nanoparticles ◽  
Prostate Specific Antigen ◽  
False Negative ◽  
Specific Antigen ◽  
Calf Serum ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay

Prostate cancer can be detected early by testing the presence of prostate-specific antigen (PSA) in the blood. Lateral flow immunoassay (LFIA) has been used because it is cost effective and easy to use and also has a rapid sample-to-answer process. Quantum dots (QDs) with very bright fluorescence have been previously used to improve the detection sensitivity of LFIAs. In the current study, a highly sensitive LFIA kit was devised using QD-embedded silica nanoparticles. In the present study, only a smartphone and a computer software program, ImageJ, were used, because the developed system had high sensitivity by using very bright nanoprobes. The limit of PSA detection of the developed LFIA system was 0.138 ng/mL. The area under the curve of this system was calculated as 0.852. The system did not show any false-negative result when 47 human serum samples were analyzed; it only detected PSA and did not detect alpha-fetoprotein and newborn calf serum in the samples. Additionally, fluorescence was maintained on the strip for 10 d after the test. With its high sensitivity and convenience, the devised LFIA kit can be used for the diagnosis of prostate cancer.

Extremely high values of prostate-specific antigen in patients with adenocarcinoma of the prostate; demonstration of the "hook effect".

1988 ◽  
Vol 34 (10) ◽  
pp. 2175-2177 ◽  
Author(s):  
H C Vaidya ◽  
B A Wolf ◽  
N Garrett ◽  
W J Catalona ◽  
R V Clayman ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Prostate Specific Antigen ◽  
Case Reports ◽  
Specific Antigen ◽  
Hook Effect ◽  
Step Procedure ◽  
Adenocarcinoma Of The Prostate ◽  
One Step ◽  
High Concentrations ◽  
The One

Abstract We reviewed 721 consecutive samples submitted for measurement of prostate-specific antigen (PSA) over five months. We identified three patients with extremely high PSA concentrations: 650, 1840, and 3280 micrograms/L (their acid phosphatase activities were 3.2, 1337, and 2.8 U/L, respectively), and present case reports for the latter two. Serial dilutions of samples obtained from the patient with the highest PSA concentration indicated that the one-step Tandem-PSA assay gave falsely low values for high concentrations of PSA, an observation consistent with the phenomenon of the "hook effect." This effect was not observed when the sample was reanalyzed for PSA by a two-step procedure.

scholarly journals Development of immunoradiometric assay for quantitative determination of free prostate-specific antigen

2005 ◽  
Vol 24 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Miroslava Jankovic ◽  
Maja Kosanovic ◽  
Ljiljana Hajdukovic-Dragojlovic ◽  
Snezana Golubovic
Keyword(s):  
Quantitative Determination ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Laboratory Diagnostics ◽  
Immunoradiometric Assay ◽  
Serum Samples ◽  
Coefficients Of Variation ◽  
One Step ◽  
Prostate Diseases

In this study we reported the development and analytical validation of new assay for quantitative determination of free prostate-specific antigen, fPSA. It is formulated as one step, two-site "sandwich" immunoradiometric assay. Specificity of this assay was achieved by using epitope-1-reactive anti-fPSA antibody as tracer antibody. Assay was calibrated against first international standard 96/668, and its detection limit was determined as 0.08 mg/L. Intra- and inter-assay coefficients of variation were 3.42-7.53% and 7.04-8.33%, respectively. Measured concentrations of serially diluted serum samples were close to the calculated concentrations, indicating good linearity with recovery percentage ranging from 98.7-107.4%. Analytical performance characteristics of fPSA assay speaks in favor of its use as a reliable tool in laboratory diagnostics relating to prostate diseases.

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