A molecular-beacon-based asymmetric PCR assay for easy visualization of amplicons in the diagnosis of trichomoniasis

2016 ◽  
Vol 86 ◽  
pp. 41-47 ◽  
Author(s):  
Subash C. Sonkar ◽  
Divya Sachdev ◽  
Prashant K. Mishra ◽  
Anita Kumar ◽  
Pratima Mittal ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Asymmetric Pcr ◽  
Pcr Assay

scholarly journals A molecular‐beacon‐based asymmetric PCR assay for detecting polymorphisms related to folate metabolism

2020 ◽  
Vol 34 (8) ◽  
Author(s):  
Qi Peng ◽  
Shengbin Liao ◽  
Yuejing He ◽  
Liyun Liang ◽  
Xiaohua Su ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Folate Metabolism ◽  
Asymmetric Pcr ◽  
Pcr Assay

Rapid real-time PCR assay for detecting Salmonella in raw and ready-to-eat meats

2008 ◽  
Vol 56 (4) ◽  
pp. 451-458 ◽  
Author(s):  
Jitu Patel ◽  
Arvind Bhagwat
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pathogen Detection ◽  
Molecular Beacon ◽  
False Negative ◽  
Detection Methods ◽  
Time Data ◽  
Pcr Assay ◽  
Serovar Typhimurium ◽  
Meat Samples

A real-time PCR assay was evaluated for the rapid detection (10 h) ofSalmonellain meats using molecular beacon probes available as a commercial kit (iQ-Check, Bio-Rad laboratories). Raw (chicken, pork) and ready-to-eat (RTE) meats were artificially contaminated withSalmonella entericaserovar Typhimurium at the estimated level of 2 to 4 cells per 25 g. After 8 h of pre-enrichment in buffered peptone water, a molecular beacon-based PCR assay was performed to detect contamination in raw and RTE meats. The sensitivity and accuracy of the assay were compared with the conventional USDA microbiological procedure. Comparative evaluation of the USDA procedure with the rapid PCR assay for meat samples (n = 63) revealed 1 false negative pork sample with the PCR assay. All uninoculated controls (n = 34) but one sample were negative by both the 10-h PCR assay and the USDA procedure. Developing rapid pathogen detection methods with shorter pre-enrichment times (8-h) and real-time data monitoring capabilities will benefit the industry in preventing recall of contaminated meats by stopping the contaminated products from being introduced into the marketplace.

scholarly journals Evaluation of a Multiplex PCR Assay To Rapidly Detect Enterobacteriaceae with a Broad Range of β-Lactamases Directly from Perianal Swabs

2016 ◽  
Vol 60 (11) ◽  
pp. 6957-6961 ◽  
Author(s):  
Kalyan D. Chavda ◽  
Michael J. Satlin ◽  
Liang Chen ◽  
Claudia Manca ◽  
Stephen G. Jenkins ◽  
...  
Keyword(s):  
Hematopoietic Stem Cell Transplant ◽  
Molecular Beacon ◽  
Cell Transplant ◽  
Transplant Recipients ◽  
Pcr Assay ◽  
Hematopoietic Stem ◽  
Content Type ◽  
Multiplex Pcr Assay ◽  
Highly Sensitive ◽  
Chromogenic Agar

ABSTRACTWe developed and evaluated multiplexed molecular beacon probes in a real-time PCR assay to identify prominent extended-spectrum-β-lactamase, plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemase genes directly from perianal swab specimens within 6 h. We evaluated this assay on 158 perianal swabs collected from hematopoietic stem cell transplant recipients and found that this assay was highly sensitive and specific for detection of CTX-M-, pAmpC-, and KPC-producingEnterobacteriaceaecompared to culture on chromogenic agar.

scholarly journals Molecular Beacon-Based Homogeneous Fluorescence PCR Assay for the Diagnosis of Infectious Diseases.

2000 ◽  
Vol 16 (2) ◽  
pp. 245-248 ◽  
Author(s):  
Qing-Ge LI ◽  
Ji-Xuan LIANG ◽  
Guo-Yan LUAN ◽  
Yang ZHANG ◽  
Kun WANG
Keyword(s):  
Infectious Diseases ◽  
Molecular Beacon ◽  
Pcr Assay
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