Symmetric vs asymmetric PCR and molecular beacon probe in the detection of a target gene of adenovirus

2000 ◽  
Vol 14 (1) ◽  
pp. 25-32 ◽  
Author(s):  
SK Poddar
Keyword(s):  
Target Gene ◽  
Molecular Beacon ◽  
Asymmetric Pcr

scholarly journals A molecular‐beacon‐based asymmetric PCR assay for detecting polymorphisms related to folate metabolism

2020 ◽  
Vol 34 (8) ◽  
Author(s):  
Qi Peng ◽  
Shengbin Liao ◽  
Yuejing He ◽  
Liyun Liang ◽  
Xiaohua Su ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Folate Metabolism ◽  
Asymmetric Pcr ◽  
Pcr Assay

A molecular-beacon-based asymmetric PCR assay for easy visualization of amplicons in the diagnosis of trichomoniasis

2016 ◽  
Vol 86 ◽  
pp. 41-47 ◽  
Author(s):  
Subash C. Sonkar ◽  
Divya Sachdev ◽  
Prashant K. Mishra ◽  
Anita Kumar ◽  
Pratima Mittal ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Asymmetric Pcr ◽  
Pcr Assay

scholarly journals Rapid Detection ofFKS-Associated Echinocandin Resistance in Candida glabrata

2016 ◽  
Vol 60 (11) ◽  
pp. 6573-6577 ◽  
Author(s):  
Yanan Zhao ◽  
Yoji Nagasaki ◽  
Milena Kordalewska ◽  
Ellen G. Press ◽  
Ryan K. Shields ◽  
...  
Keyword(s):  
Candida Glabrata ◽  
Molecular Beacon ◽  
Rapid Identification ◽  
Molecular Diagnostic ◽  
Wild Type ◽  
Asymmetric Pcr ◽  
Content Type ◽  
Allele Specific ◽  
Echinocandin Resistance

ABSTRACTA novel and highly accurate diagnostic assay platform was established for rapid identification ofFKSmutations associated with echinocandin resistance inCandida glabrata. The assay platform uses allele-specific molecular beacon and DNA melt analysis following asymmetric PCR. A dual assay forFKS1andFKS2was developed to identify within 3 h the most common and clinically relevant resistance-associated mutations, including 8FKS1HS1 (wild type [WT], S629P, F625S, D632Y, D632E [T1896G], D632E [T1896A], I634V, and F625F) and 7FKS2HS1 (WT, F659del, F659S, F659V, F659L, S663P, and S663F) genotypes. A blinded panel of 188C. glabrataclinical isolates was tested by both assays. The molecular diagnostic results from the dual assay were 100% concordant with data obtained from DNA sequencing. This platform has the potential to overcome the deficiencies of existingin vitrosusceptibility-based assays to identify echinocandin-resistantC. glabrataand holds promise as a surrogate diagnostic method to better direct echinocandin therapy.

Investigating miR-125b target gene Arid3a in trisomy 21-associated leukemogenesis

2020 ◽  
Author(s):  
K Weigert ◽  
O Alejo-Valle ◽  
M Labuhn ◽  
V Amstislavskiy ◽  
S Emmrich ◽  
...  
Keyword(s):  
Trisomy 21 ◽  
Target Gene

Suppression of 11[beta]-hydroxysteroid dehydrogenase type 1 target gene regulation by hypoxia

2016 ◽  
Author(s):  
Bushra Shammout ◽  
Adewonuola Alase ◽  
Miriam Wittmann ◽  
Paul Stewart ◽  
Ana Tiganescu
Keyword(s):  
Gene Regulation ◽  
Target Gene ◽  
Hydroxysteroid Dehydrogenase

2049-P: The Chd4 Helicase of the Nucleosome Remodeling and Deacetylase Complex Modulates Pdx1 Target Gene Expression In Vitro

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 2049-P
Author(s):  
REBECCA K. DAVIDSON ◽  
NOLAN CASEY ◽  
JASON SPAETH
Keyword(s):  
Gene Expression ◽  
Target Gene ◽  
Nucleosome Remodeling ◽  
Target Gene Expression
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