Water-soluble gold nanoclusters prepared by protein-ligand interaction as fluorescent probe for real-time assay of pyrophosphatase activity

2016 ◽  
Vol 83 ◽  
pp. 1-8 ◽  
Author(s):  
Hao-Hua Deng ◽  
Fei-Fei Wang ◽  
Xiao-Qiong Shi ◽  
Hua-Ping Peng ◽  
Ai-Lin Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Gold Nanoclusters ◽  
Water Soluble ◽  
Ligand Interaction ◽  
Protein Ligand Interaction ◽  
Pyrophosphatase Activity

Real-time imaging of alkaline phosphatase activity of diabetes in mice via a near-infrared fluorescent probe

2021 ◽  
Author(s):  
Wen-Xin Wang ◽  
Wen-Li Jiang ◽  
Hong Guo ◽  
Yongfei Li ◽  
Chun-Yan Li
Keyword(s):  
Alkaline Phosphatase ◽  
Real Time ◽  
Fluorescent Probe ◽  
Alkaline Phosphatase Activity ◽  
Near Infrared ◽  
Water Soluble ◽  
Alp Activity ◽  
Diabetes In Mice ◽  
Real Time Image ◽  
Time Image

A novel water-soluble near-infrared fluorescent probe named QX-P with simple synthesis is developed for detecting ALP. The probe can not only visualize ALP activity in four cell lines, but also real-time image ALP activity of diabetes in mice.

Nitrogen and Sulfur Co-doped Fluorescent Carbon Dots for the Detection of Morin and Cell Imaging

2018 ◽  
Vol 15 (1) ◽  
pp. 47-55
Author(s):  
Xuebing Li ◽  
Haifen Yang ◽  
Ning Wang ◽  
Tijian Sun ◽  
Wei Bian ◽  
...  
Keyword(s):  
Microwave Heating ◽  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Water Soluble ◽  
Heating Process ◽  
X Ray ◽  
Doped Carbon Dots ◽  
Co Doped

Background: Morin has many pharmacological functions including antioxidant, anticancer, anti-inflammatory, and antibacterial effects. It is commonly used in the treatment of antiviral infection, gastropathy, coronary heart disease and hepatitis B in clinic. However, researches have shown that morin is likely to show prooxidative effects on the cells when the amount of treatment is at high dose, leading to the decrease of intracellular ATP levels and the increase of necrosis process. Therefore, it is necessary to determine the concentration of morin in biologic samples. Method: Novel water-soluble and green nitrogen and sulfur co-doped carbon dots (NSCDs) were prepared by a microwave heating process with citric acid and L-cysteine. The fluorescence spectra were collected at an excitation wavelength of 350 nm when solutions of NSCDs were mixed with various concentrations of morin. Results: The as-prepared NSCDs were characterized by transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The fluorescence intensity of NSCDs decreased significantly with the increase of morin concentration. The fluorescence intensity of NSCDs displayed a linear response to morin in the concentration 0.10-30 μM with a low detection limit of 56 nM. The proposed fluorescent probe was applied to analysis of morin in human body fluids with recoveries of 98.0-102%. Conclusion: NSCDs were prepared by a microwave heating process. The present analytical method is sensitive to morin. The quenching process between NSCDs and morin is attributed to the static quenching. In addition, the cellular toxicity on HeLa cells indicated that the as-prepared NSCDs fluorescent probe does not show obvious cytotoxicity in cell imaging. Our proposed method possibly opens up a rapid and nontoxic way for preparing heteroatom doped carbon dots with a broad application prospect.

scholarly journals Two Rules on the Protein-Ligand Interaction

2008 ◽  
Author(s):  
Xiaodong Pang ◽  
Linxiang Zhou ◽  
Lily Zhang ◽  
Lina Xu ◽  
Xinyi Zhang
Keyword(s):  
Ligand Interaction ◽  
Protein Ligand Interaction

scholarly journals Protein-ligand free energies of binding from full-protein DFT calculations: convergence and choice of exchange-correlation functional

2021 ◽  
Author(s):  
Lennart Gundelach ◽  
Christofer S Tautermann ◽  
Thomas Fox ◽  
Chris-Kriton Skylaris
Keyword(s):  
Drug Discovery ◽  
Ligand Binding ◽  
Dft Calculations ◽  
Quantum Mechanical ◽  
Free Energies ◽  
Ligand Interaction ◽  
Exchange Correlation ◽  
Ligand Free ◽  
Protein Ligand Interaction ◽  
Binding Free Energies

The accurate prediction of protein-ligand binding free energies with tractable computational methods has the potential to revolutionize drug discovery. Modeling the protein-ligand interaction at a quantum mechanical level, instead of...

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