Fluorescence detection of telomerase activity in cancer cell extracts based on autonomous exonuclease III-assisted isothermal cycling signal amplification

2016 ◽  
Vol 83 ◽  
pp. 102-105 ◽  
Author(s):  
Caifeng Ding ◽  
Xiaoqian Li ◽  
Wei Wang ◽  
Yaoyao Chen
Keyword(s):  
Cancer Cell ◽  
Fluorescence Detection ◽  
Signal Amplification ◽  
Telomerase Activity ◽  
Exonuclease Iii ◽  
Cell Extracts

Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6689-6697 ◽  
Author(s):  
Han Zhao ◽  
Ying Yan ◽  
Mingjian Chen ◽  
Tingting Hu ◽  
Kefeng Wu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Fluorescent Method ◽  
Polynucleotide Kinase ◽  
Amplification Strategy

A facile fluorescent method has been developed for polynucleotide kinase detection based on copper nanoparticles and exonuclease III-assisted signal amplification.

DNA-fueled molecular machine for label-free and non-enzymatic ultrasensitive detection of telomerase activity

The Analyst ◽  
2016 ◽  
Vol 141 (16) ◽  
pp. 4855-4858 ◽  
Author(s):  
Panpan Sun ◽  
Xiang Ran ◽  
Chaoqun Liu ◽  
Chaoying Liu ◽  
Fang Pu ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cell ◽  
Telomerase Activity ◽  
Molecular Machine ◽  
Ultrasensitive Detection ◽  
Single Cell Level ◽  
Label Free ◽  
Enzymatic Method ◽  
Cell Level ◽  
Cell Extracts

A label-free and non-enzymatic method based on DNA-fueled molecular machine has been introduced for ultrasensitive detection of telomerase activity in cancer cell extracts even at the single-cell level.

Label-free and highly sensitive fluorescence detection of lead(ii) based on DNAzyme and exonuclease III-assisted cascade signal amplification

2019 ◽  
Vol 43 (15) ◽  
pp. 5857-5862 ◽  
Author(s):  
Jiafeng Pan ◽  
Qiong Li ◽  
Danhua Zhou ◽  
Junhua Chen
Keyword(s):  
Fluorescence Detection ◽  
Signal Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Highly Sensitive ◽  
Exo Iii

A Pb2+ biosensor has been constructed based on Exo III-assisted cascade signal amplification using 2-amino-5,6,7-trimethyl-1,8-naphthyridine as the signal indicator.

Gastrin regulates growth of human pancreatic cancer in a tonic and autocrine fashion

1996 ◽  
Vol 270 (5) ◽  
pp. R1078-R1084 ◽  
Author(s):  
J. P. Smith ◽  
A. Shih ◽  
Y. Wu ◽  
P. J. McLaughlin ◽  
I. S. Zagon
Keyword(s):  
Gene Expression ◽  
Pancreatic Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Pancreatic Cancer Cell ◽  
Cancer Cell Lines ◽  
Human Pancreatic Cancer ◽  
Growth Media ◽  
Human Pancreatic Cancer Cell ◽  
Cell Extracts

The gastrointestinal peptides gastrin and cholecystokinin (CCK) stimulate growth of human pancreatic cancer through a CCK-B/gastrin- like receptor. In the present study we evaluated whether growth of human pancreatic cancer is endogenously regulated by gastrin. Immunohistomical examination of BxPC-3 cells and tumor xenografts revealed specifc gastrin immunoreactivity. Gastrin was detected by radioimmunoassay in pancreatic cancer cell extracts and in pancreatic cancer cell extracts and in the growth media. With use of reverse-transcriptase polymerase chain reaction gastrin gene expression was detected in both cultured BxPC-3 cancer cells and transplanted tumors, as well as seven addition human pancreatic cancer cell lines. Growth of BxPC-3 human pancreatic cancer cell in serum-free medium was inhibited by the addition of the CCK-B/gastrin receptor antagonist L-365,260, and gastrin treatment reversed the inhibitory effect of the antagonist. A selective gastrin antibody (Ab repressed growth of BxPC-3 cells. Gastrin immunoreactivity was detected in fresh human pancreatic cancer specimens but not in normal human pancreatic tissue. These data provide the first evidence that growth of a human pancreatic cancer is tonically stimulated by the autocrine production of gastrin. Evidence for the ubiquity of this system was provided by the detection of gastrin gene expression in multiple human pancreatic cancer cell lines and detection of gastrin in cell lines and fresh pancreatic tumors.

scholarly journals Raloxifene Inhibits Estrogen-induced Up-regulation of Telomerase Activity in a Human Breast Cancer Cell Line

2003 ◽  
Vol 278 (44) ◽  
pp. 43363-43372 ◽  
Author(s):  
Jun Kawagoe ◽  
Masahide Ohmichi ◽  
Toshifumi Takahashi ◽  
Chika Ohshima ◽  
Seiji Mabuchi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Telomerase Activity ◽  
Human Breast Cancer Cell ◽  
Human Breast
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