Real-time monitoring of 3D cell culture using a 3D capacitance biosensor

2016 ◽  
Vol 77 ◽  
pp. 56-61 ◽  
Author(s):  
Sun-Mi Lee ◽  
Nalae Han ◽  
Rimi Lee ◽  
In-Hong Choi ◽  
Yong-Beom Park ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
3D Cell Culture ◽  
Real Time Monitoring

scholarly journals A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 588 ◽  
Author(s):  
Jeong Hwa Kim ◽  
Ju Young Park ◽  
Songwan Jin ◽  
Sik Yoon ◽  
Jong-Young Kwak ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Microfluidic Chip ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Cell Culture ◽  
Culture Conditions ◽  
Real Time Monitoring ◽  
Nanofiber Scaffold

Recently, three-dimensional (3D) cell culture and tissue-on-a-chip application have attracted attention because of increasing demand from the industries and their potential to replace conventional two-dimensional culture and animal tests. As a result, numerous studies on 3D in-vitro cell culture and microfluidic chip have been conducted. In this study, a microfluidic chip embracing a nanofiber scaffold is presented. A electrospun nanofiber scaffold can provide 3D cell culture conditions to a microfluidic chip environment, and its perfusion method in the chip can allow real-time monitoring of cell status based on the conditioned culture medium. To justify the applicability of the developed chip to 3D cell culture and real-time monitoring, HepG2 cells were cultured in the chip for 14 days. Results demonstrated that the cells were successfully cultured with 3D culture-specific-morphology in the chip, and their albumin and alpha-fetoprotein production was monitored in real-time for 14 days.

scholarly journals Transistor in a tube: A route to three-dimensional bioelectronics

2018 ◽  
Vol 4 (10) ◽  
pp. eaat4253 ◽  
Author(s):  
C. Pitsalidis ◽  
M. P. Ferro ◽  
D. Iandolo ◽  
L. Tzounis ◽  
S. Inal ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Conducting Polymer ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Biological Tissues ◽  
Tissue Formation ◽  
Polymer Scaffold ◽  
Real Time Monitoring ◽  
Electrochemical Transistor

Advances in three-dimensional (3D) cell culture materials and techniques, which more accurately mimic in vivo systems to study biological phenomena, have fostered the development of organ and tissue models. While sophisticated 3D tissues can be generated, technology that can accurately assess the functionality of these complex models in a high-throughput and dynamic manner is not well adapted. Here, we present an organic bioelectronic device based on a conducting polymer scaffold integrated into an electrochemical transistor configuration. This platform supports the dual purpose of enabling 3D cell culture growth and real-time monitoring of the adhesion and growth of cells. We have adapted our system to a 3D tubular geometry facilitating free flow of nutrients, given its relevance in a variety of biological tissues (e.g., vascular, gastrointestinal, and kidney) and processes (e.g., blood flow). This biomimetic transistor in a tube does not require photolithography methods for preparation, allowing facile adaptation to the purpose. We demonstrate that epithelial and fibroblast cells grow readily and form tissue-like architectures within the conducting polymer scaffold that constitutes the channel of the transistor. The process of tissue formation inside the conducting polymer channel gradually modulates the transistor characteristics. Correlating the real-time changes in the steady-state characteristics of the transistor with the growth of the cultured tissue, we extract valuable insights regarding the transients of tissue formation. Our biomimetic platform enabling label-free, dynamic, and in situ measurements illustrates the potential for real-time monitoring of 3D cell culture and compatibility for use in long-term organ-on-chip platforms.

scholarly journals A Modular Toolkit to Fabricate Microfluidic Devices for 3D Cell Culture and Near Real-time Measurements

2020 ◽  
Author(s):  
Giraso Kabandana ◽  
Adam Michael Ratajczak ◽  
Chengpeng Chen
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Low Cost ◽  
New Technology ◽  
Microfluidic Channel ◽  
3D Cell Culture ◽  
Microfluidic Devices ◽  
In Vitro Cell Cultures ◽  
Scoring Tools

Microfluidic technology has tremendously facilitated the development of in vitro cell cultures and studies. Conventionally, microfluidic devices are fabricated with extensive facilities by well-trained researchers, which hinders the widespread adoption of the technology for broader applications. Enlightened by the fact that low-cost microbore tubing is a natural microfluidic channel, we developed a series of adaptors in a toolkit that can twine, connect, organize, and configure the tubing to produce functional microfluidic units. Three subsets of the toolkit were thoroughly developed: the tubing and scoring tools, the flow adaptors, and the 3D cell culture suite. To demonstrate the usefulness and versatility of the toolkit, we assembled a microfluidic device and successfully applied it for 3D macrophage cultures, flow-based stimulation, and automated near real-time quantitation with new knowledge generated. Overall, we present a new technology that allows simple, fast, and robust assembly of customizable and scalable microfluidic devices with minimal facilities, which is broadly applicable to research that needs or could be enhanced by microfluidics.

scholarly journals Real-time monitoring of specific oxygen uptake rates of embryonic stem cells in a microfluidic cell culture device

2016 ◽  
Vol 11 (9) ◽  
pp. 1179-1189 ◽  
Author(s):  
Alexandre Super ◽  
Nicolas Jaccard ◽  
Marco Paulo Cardoso Marques ◽  
Rhys Jarred Macown ◽  
Lewis Donald Griffin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Embryonic Stem Cells ◽  
Oxygen Uptake ◽  
Real Time ◽  
Embryonic Stem ◽  
Real Time Monitoring ◽  
Uptake Rates ◽  
Microfluidic Cell Culture

Generic Raman-based calibration models enabling real-time monitoring of cell culture bioreactors

2015 ◽  
Vol 31 (4) ◽  
pp. 1004-1013 ◽  
Author(s):  
Hamidreza Mehdizadeh ◽  
David Lauri ◽  
Krizia M. Karry ◽  
Mojgan Moshgbar ◽  
Renee Procopio-Melino ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Real Time Monitoring ◽  
Calibration Models
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