A universal and label-free aptasensor for fluorescent detection of ATP and thrombin based on SYBR Green Idye

2013 ◽  
Vol 42 ◽  
pp. 193-197 ◽  
Author(s):  
Ling Kong ◽  
Jin Xu ◽  
Yunying Xu ◽  
Yun Xiang ◽  
Ruo Yuan ◽  
...  
Keyword(s):  
Sybr Green ◽  
Fluorescent Detection ◽  
Label Free

scholarly journals Simple and Label-Free Fluorescent Detection of Melamine Based on Melamine–Thymine Recognition

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 2968 ◽  
Author(s):  
Hualin Yang ◽  
Jiujun Wang ◽  
Qinghua Wu ◽  
Yun Wang ◽  
Li Li ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Chain Structure ◽  
Sybr Green ◽  
Sybr Green I ◽  
Fluorescent Detection ◽  
Fluorescence Signal ◽  
Label Free ◽  
Protein Levels ◽  
Fluorescent Method ◽  
Milk Adulteration

In the past few years, melamine has been illegally added into dairy products to increase the apparent crude protein levels. If humans or animals drink the milk adulteration of melamine, it can form insoluble melamine–cyanurate crystals in their kidneys which causes kidney damage or even death. In the present work, we constructed a simple and label-free fluorescent method for melamine detection based on melamine-thymine recognition. SYBR Green I was utilized as a reporter for this method as it did not require any modification or expensive equipment. In the absence of melamine, polythymine DNA was digested by Exo I, which caused a decrease in the fluorescence signal. In the presence of melamine, the polythymine DNA was able to fold into a double chain structure, however this was done with the help of T-melamine-T mismatches to prevent degradation. Then, the SYBR Green I combined with the double-stranded DNA to result in an intense fluorescence signal. The limit of detection in this method was 1.58 μM, which satisfied the FDA standards. This method also had a good linear relationship within the range of 10–200 μM. In addition, this new method has a good selectivity to distinguish melamine from the component of milk. As a result, we developed a simple and highly selectivity method for melamine detection.

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

A label-free aptasensor for turn-on fluorescent detection of ochratoxin A based on aggregation-induced emission probe

2019 ◽  
Vol 8 (1) ◽  
pp. 015003 ◽  
Author(s):  
Lei Lv ◽  
Chengbi Cui ◽  
Wancui Xie ◽  
Wenyi Sun ◽  
Shuang Ji ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fluorescent Detection ◽  
Label Free ◽  
Aggregation Induced Emission ◽  
Turn On

Label-Free Fluorescent Detection of Hg2+ in Aqueous Media Based on N-Doped MoS2 Nanosheets

NANO ◽  
2018 ◽  
Vol 13 (05) ◽  
pp. 1850057 ◽  
Author(s):  
Jing Liu ◽  
Xiao Li ◽  
Xin Gong ◽  
Taiping Qing ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Lake Water ◽  
Water Samples ◽  
Low Cost ◽  
Aqueous Media ◽  
Fluorescent Detection ◽  
Label Free ◽  
Mos2 Nanosheets ◽  
Practical Applications ◽  
Dopant Atoms

The incorporation of dopant atoms alters the structure of MoS2, resulting in unique properties and practical applications. Herein, a facile and low-cost approach for producing N-doped molybdenum disulfide (N-MoS[Formula: see text] was carried out using melamine and MoS2 in a laboratory tube furnace at 400[Formula: see text]C for 2[Formula: see text]h. The N-MoS2 fluorescent probe showed sensitive response to Hg[Formula: see text] in a wide concentration range of 0.4–10[Formula: see text][Formula: see text]M with a low limit of 28[Formula: see text]nM. The practical applicability of detecting Hg[Formula: see text] in lake water samples may open a new way to evaluate Hg[Formula: see text] in wastewater.

An enzyme-free and label-free assay for copper(ii) ion detection based on self-assembled DNA concatamers and Sybr Green I

The Analyst ◽  
2013 ◽  
Vol 138 (17) ◽  
pp. 4737 ◽  
Author(s):  
Chenchen Ge ◽  
Junhua Chen ◽  
Wei Wu ◽  
Zhiyuan Fang ◽  
Lingbo Chen ◽  
...  
Keyword(s):  
Sybr Green ◽  
Sybr Green I ◽  
Label Free ◽  
Ion Detection ◽  
Self Assembled

Improved Detection of Hg2+ in Aqueous Solution with High Sensitivity and Selectivity by Using Mercury-Specific DNA, Sybr Green I and Gold Nanoparticles

2011 ◽  
Vol 239-242 ◽  
pp. 934-939
Author(s):  
Hui Xu ◽  
Shuli Gao ◽  
Jian Nong Chen ◽  
Quan Wen Liu
Keyword(s):  
Gold Nanoparticles ◽  
High Sensitivity ◽  
Sybr Green ◽  
Sybr Green I ◽  
Fluorescence Signal ◽  
Potassium Ions ◽  
Label Free ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Fluorescence Turn On

We report a label-free, fast, fluorescence turn on assay for Hg2+detecton by using mercury-specific DNA (MSD), Sybr Green I (SG) and gold nanoparticles (AuNPs). SG efficiently discriminates MSD and MSD/Hg2+complex. The addition of gold nanoparticle decreases the background fluorescence signal further for MSD. The fluorescence intensity of MSD/Hg2+complex keeps constant after addition of AuNPs. This property improves the signal-to-background ratio and decreases the detection limitation further. In addition, the method shows improved selectivity compared with that in the absence of AuNPs. This strategy could be applied to the detection of potassium ions and showed good generality.

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