Alizarin red S/copper ion-based ensemble for fluorescence turn on detection of glutathione with tunable dynamic range

2012 ◽  
Vol 38 (1) ◽  
pp. 202-208 ◽  
Author(s):  
Zhanguang Chen ◽  
Zhen Wang ◽  
Junhui Chen ◽  
Xi Chen
Keyword(s):  
Dynamic Range ◽  
Copper Ion ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Turn On ◽  
Fluorescence Turn On

Assembly of alizarin red S/boric acid ultrathin films based on layered double hydroxide for fluorescence turn on detection of tiopronin

2016 ◽  
Vol 4 (16) ◽  
pp. 3415-3421 ◽  
Author(s):  
Haiqin Wu ◽  
Lan Jin
Keyword(s):  
Boric Acid ◽  
Layered Double Hydroxide ◽  
Ultrathin Films ◽  
High Selectivity ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Double Hydroxide

New facile, economical and fluorescent (ARS–H3BO3/LDH)n UTFs were developed, exhibiting response to tiopronin with high selectivity, precision and reversibility.

scholarly journals Ultralarge Modulation of Single Wall Carbon Nanotube Fluorescence Mediated by Neuromodulators Adsorbed on Arrays of Oligonucleotide Rings

2018 ◽  
Author(s):  
Abraham G. Beyene ◽  
Ali A. Alizadehmojarad ◽  
Gabriel Dorlhiac ◽  
Aaron M. Streets ◽  
Petr Král ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Single Wall Carbon Nanotubes ◽  
Single Wall Carbon ◽  
Turn On ◽  
Sensing Applications ◽  
Fluorescence Modulation ◽  
Dynamics Simulations ◽  
Experimental Findings ◽  
Fluorescence Turn On

AbstractNon-covalent interactions between single-stranded DNA (ssDNA) oligonucleotides and single wall carbon nanotubes (SWNTs) have provided a unique class of tunable chemistries for a variety of applications. However, mechanistic insight into both the photophysical and intermolecular phenomena underlying their utility is lacking, resulting in obligate heuristic approaches for producing ssDNA-SWNT based technologies. In this work, we present an ultrasensitive “turn-on” nanosensor for neuromodulators dopamine and norepinephrine with strong ΔF/F0 of up to 3500%, a signal appropriate for in vivo imaging, and uncover the photophysical principles and intermolecular interactions that govern the molecular recognition and fluorescence modulation of this nanosensor synthesized from the non-covalent conjugation of (GT)6 ssDNA strands on SWNTs. The fluorescence modulation of the ssDNA-SWNT conjugate is shown to exhibit remarkable sensitivity to the ssDNA sequence chemistry, length, and surface density, providing a wealth of parameters with which to tune nanosensor dynamic range and strength of fluorescence turn-on. We employ classical and quantum mechanical molecular dynamics simulations to rationalize our experimental findings. Calculations show that (GT)6 ssDNA form ordered loops around SWNT, inducing periodic surface potentials that modulate exciton recombination lifetimes. Further evidence is presented to elucidate how analyte binding modulates SWNT fluorescence. We discuss the implications of our findings for SWNT-based molecular sensing applications.

DNA/Ligand/Ion-Based Ensemble for Fluorescence Turn on Detection of Cysteine and Histidine with Tunable Dynamic Range

2010 ◽  
Vol 82 (19) ◽  
pp. 8211-8216 ◽  
Author(s):  
Fang Pu ◽  
Zhenzhen Huang ◽  
Jinsong Ren ◽  
Xiaogang Qu
Keyword(s):  
Dynamic Range ◽  
Turn On ◽  
Fluorescence Turn On

A Fluorescence Turn-On Probe for Thiols with a Tunable Dynamic Range

2016 ◽  
Vol 26 (3) ◽  
pp. 1077-1081 ◽  
Author(s):  
Qian Li ◽  
Rui Guo ◽  
Weiying Lin
Keyword(s):  
Dynamic Range ◽  
Turn On ◽  
Fluorescence Turn On

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

2018 ◽  
Author(s):  
Suying Xu ◽  
Adam Sedgwick ◽  
Souad Elfecky ◽  
Wenbo Chen ◽  
Ashley Jones ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Boronic Acid ◽  
Binding Constants ◽  
Strong Fluorescence ◽  
Fluorescence Response ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Hydrogel System

<p>A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection<i>. </i>In solution, the fluorescent probe<b> </b>displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M). <b><u></u></b></p>

Simple Fluorescence Turn-On Chemosensor for Selective Detection of Ba2+ Ion and Its Live Cell Imaging

2019 ◽  
Vol 91 (15) ◽  
pp. 10095-10101 ◽  
Author(s):  
Palanisamy Ravichandiran ◽  
Sivakumar Allur Subramaniyan ◽  
Antony Paulraj Bella ◽  
Princy Merlin Johnson ◽  
Ae Rhan Kim ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On

scholarly journals Globular adiponectin inhibits osteoblastic differentiation of vascular smooth muscle cells through the PI3K/AKT and Wnt/β-catenin pathway

2021 ◽  
Author(s):  
Yun Zhou ◽  
Li-Long Wei ◽  
Rui-Ping Zhang ◽  
Cheng-Wu Han ◽  
Yongtong Cao
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Osteoblastic Differentiation ◽  
Alizarin Red S ◽  
Regulation Mechanism ◽  
Alizarin Red ◽  
Globular Adiponectin

AbstractLipid metabolism is closely related to the improvement of vascular calcification (VC) in chronic kidney disease (CKD). Globular adiponectin (gAd) has been reported to be involved in the development of VC in CKD, but the detailed regulatory role remains unclear. The present study is aimed to investigate the biological function and the underlying regulation mechanism of gAd in the process of VC during CKD. Vascular smooth muscle cells (VSMCs) calcification was determined by Alizarin Red S staining. Protein signaling related with VC was tested by western blotting. The expression and intracellular localization of runt-related transcription factor 2 (Runx2) was detected by immunofluorescence and uraemic rat with VC was established by a two-step nephrectomy. Combined with the results of Alizarin Red S staining, we discovered that β-glycerophosphate (β-Gp)-induced the osteoblastic differentiation of VSMCs was significantly reversed by gAd treatment. Along with the VSMCs calcification and the increase of Runx2 in β-Gp-exposed VSMCs, the activities of protein kinase B (AKT) and Wnt/β-catenin pathway were enhanced, but that were counteracted by the exposure of gAd in rat and human VSMCs. After administration with agonists of the Wnt (SKL2001) and AKT (SC79), there appeared more osteoblastic differentiation and higher expression of Runx2 in gAd-treated VSMCs, but showing lower impact in the presence of SC79 than that in the presence of SKL2001. In the in vivo experiments, intravenous injection of gAd also significantly inhibited VC and Runx2 level in uraemic rat in a dose-dependent manner, possibly through regulating Wnt/β-catenin pathway. This study demonstrates that gAd ameliorates osteoblastic differentiation of VSMCs possibly by blocking PI3K/AKT and Wnt/β-catenin signaling transduction. The findings provide an important foundation for gAd in treating VC in kidney diseases.

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