Peptide-induced fluorescence quenching of conjugated polyelectrolyte for label-free, ultrasensitive and selective assay of protease activity

2012 ◽  
Vol 34 (1) ◽  
pp. 221-226 ◽  
Author(s):  
Hongliang Fan ◽  
Xinhang Jiang ◽  
Tao Zhang ◽  
Qinhan Jin
Keyword(s):  
Fluorescence Quenching ◽  
Protease Activity ◽  
Label Free ◽  
Conjugated Polyelectrolyte

Label free Detection of Vitamin B12 Based on Fluorescence Quenching of Graphene Oxide Nanolayer

2015 ◽  
Vol 23 (10) ◽  
pp. 878-884 ◽  
Author(s):  
Javad Gholami ◽  
Mehrdad Manteghian ◽  
Alireza Badiei ◽  
Mehran Javanbakht ◽  
Hiroshi Ueda
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Vitamin B12 ◽  
Label Free ◽  
Label Free Detection

Amplified Fluorescence Quenching of a Conjugated Polyelectrolyte Mediated by Ca2+

Langmuir ◽  
2006 ◽  
Vol 22 (13) ◽  
pp. 5541-5543 ◽  
Author(s):  
Hui Jiang ◽  
Xiaoyong Zhao ◽  
Kirk S. Schanze
Keyword(s):  
Fluorescence Quenching ◽  
Conjugated Polyelectrolyte

Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of DNA

The Analyst ◽  
2016 ◽  
Vol 141 (15) ◽  
pp. 4719-4724 ◽  
Author(s):  
Yaqing Chang ◽  
Zhe Zhang ◽  
Huiqing Liu ◽  
Nan Wang ◽  
Jilin Tang
Keyword(s):  
Fluorescence Quenching ◽  
Label Free ◽  
Quenching Mechanism ◽  
Fluorescence Sensing ◽  
Single Stranded Dna ◽  
Cobalt Oxyhydroxide ◽  
Sensing Platform ◽  
Label Free Detection ◽  
Fluorescence Quenching Mechanism ◽  
Coooh Nanoflakes

In this study, we investigated the interaction of cobalt oxyhydroxide (CoOOH) nanoflakes with DNA and their fluorescence quenching mechanism of a FAM-labeled single-stranded DNA (ssDNA) probe.

A Label-Free Gold Nanocluster Fluorescent Probe for Protease Activity Monitoring

2014 ◽  
Vol 14 (6) ◽  
pp. 4029-4035 ◽  
Author(s):  
Jiajing Zhang ◽  
Zhen Zhang ◽  
Xin Nie ◽  
Zhenjiang Zhang ◽  
Xiaochun Wu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Protease Activity ◽  
Activity Monitoring ◽  
Label Free ◽  
Gold Nanocluster ◽  
Free Gold

Specific antibody-induced fluorescence quenching for the development of a directly applicable and label-free immunoassay

2014 ◽  
Vol 6 (15) ◽  
pp. 5454-5458 ◽  
Author(s):  
Xin Li ◽  
Jiawen Lei ◽  
Peiwu Li ◽  
Qi Zhang ◽  
Liangxiao Zhang ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Specific Antibody ◽  
Label Free ◽  
Binding Reaction

A simple and label-free immunoassay was proposed based on the specific antibody-analyte immune binding reaction induced by fluorescence quenching of the analyte.

Label-Free Aptamer Fluorescence Determination of Trace Pb2+ Using AuPd Nanoalloy Probe as Catalyst

2013 ◽  
Vol 631-632 ◽  
pp. 18-21 ◽  
Author(s):  
Zhi Liang Jiang ◽  
Mei Ling Tang ◽  
Qing Ye Liu ◽  
Ai Hui Liang
Keyword(s):  
Detection Limit ◽  
Fluorescence Quenching ◽  
Fluorescence Intensity ◽  
Water Samples ◽  
Catalytic Effect ◽  
Rhodamine 6G ◽  
Label Free ◽  
Double Stranded Dna ◽  
Fluorescence Determination

In the condition of 1.24 mmol/L EDTANa2, 16.7 mmol/L NaCl and 0.17 mmol/L Tris, the substrate chain of double-stranded DNA (dsDNA) could be cracked by Pb2+ to release single-stranded DNA (ssDNA) that adsorb onto AuPd nanoparticle (AuPdNP) and form stable AuPdNP-ssDNA, but the dsDNA can not protect AuPdNP that were aggregated to big AuPdNP aggregations (AuPdNPA) under the action of NaCl. The AuPdNP-ssDNA and AuPdNPA could be separated by centrifugation. With the concentration of Pb2+ increased, the released ssDNA increased, the AuPdNP-ssDNA in centrifugation solution increased and the catalytic effect enhanced on the fluorescence quenching reaction of Rhodamine 6G (Rh6G) and NaH2PO2, which led the fluorescence intensity at 552nm to decrease. The decreased fluorescence intensity (ΔF552nm) was linear to the concentration of Pb2+ in the range of 0.33-8.00 nmol/L, a detection limit of 0.21 nmol/L. The proposed method was applied to detect Pb2+ in water samples, with satisfactory results.

scholarly journals Quantitative and Label-Free Technique for Measuring Protease Activity and Inhibition using a Microfluidic Cantilever Array

Nano Letters ◽  
2008 ◽  
Vol 8 (9) ◽  
pp. 2968-2974 ◽  
Author(s):  
Digvijay A. Raorane ◽  
Mark D. Lim ◽  
Fanqing Frank Chen ◽  
Charles S. Craik ◽  
Arun Majumdar
Keyword(s):  
Protease Activity ◽  
Label Free ◽  
Cantilever Array

Detection of glutaraldehyde in aqueous environments based on fluorescence quenching of a conjugated polymer with pendant protonated primary amino groups

2017 ◽  
Vol 5 (20) ◽  
pp. 5010-5017 ◽  
Author(s):  
Kesong Miao ◽  
Heng Zhang ◽  
Lijuan Sun ◽  
Zhengtao Zhu ◽  
Li-Juan Fan
Keyword(s):  
Fluorescence Quenching ◽  
Conjugated Polymer ◽  
Selective Detection ◽  
Amino Groups ◽  
Conjugated Polyelectrolyte ◽  
Aqueous Environments ◽  
Primary Amino

A new conjugated polyelectrolyte was synthesized and demonstrated to be very effective in selective detection of glutaraldehyde in aqueous environments.

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