Specific antibody-induced fluorescence quenching for the development of a directly applicable and label-free immunoassay

2014 ◽  
Vol 6 (15) ◽  
pp. 5454-5458 ◽  
Author(s):  
Xin Li ◽  
Jiawen Lei ◽  
Peiwu Li ◽  
Qi Zhang ◽  
Liangxiao Zhang ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Specific Antibody ◽  
Label Free ◽  
Binding Reaction

A simple and label-free immunoassay was proposed based on the specific antibody-analyte immune binding reaction induced by fluorescence quenching of the analyte.

Label free Detection of Vitamin B12 Based on Fluorescence Quenching of Graphene Oxide Nanolayer

2015 ◽  
Vol 23 (10) ◽  
pp. 878-884 ◽  
Author(s):  
Javad Gholami ◽  
Mehrdad Manteghian ◽  
Alireza Badiei ◽  
Mehran Javanbakht ◽  
Hiroshi Ueda
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Vitamin B12 ◽  
Label Free ◽  
Label Free Detection

Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of DNA

The Analyst ◽  
2016 ◽  
Vol 141 (15) ◽  
pp. 4719-4724 ◽  
Author(s):  
Yaqing Chang ◽  
Zhe Zhang ◽  
Huiqing Liu ◽  
Nan Wang ◽  
Jilin Tang
Keyword(s):  
Fluorescence Quenching ◽  
Label Free ◽  
Quenching Mechanism ◽  
Fluorescence Sensing ◽  
Single Stranded Dna ◽  
Cobalt Oxyhydroxide ◽  
Sensing Platform ◽  
Label Free Detection ◽  
Fluorescence Quenching Mechanism ◽  
Coooh Nanoflakes

In this study, we investigated the interaction of cobalt oxyhydroxide (CoOOH) nanoflakes with DNA and their fluorescence quenching mechanism of a FAM-labeled single-stranded DNA (ssDNA) probe.

A label-free approach to kinetic analysis and high multiplex detection of targeted drugs with phase surface plasmon resonance imaging

2015 ◽  
Vol 7 (5) ◽  
pp. 1738-1744 ◽  
Author(s):  
Yijia Wang ◽  
Chonglei Zhang ◽  
Yuquan Zhang ◽  
Hui Fang ◽  
Changjun Min ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Targeted Drugs ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Multiplex Detection ◽  
Resonance Imaging ◽  
Binding Reaction ◽  
Phase Surface

Phase SPRi biosensor with ability of high multiplex detection was applied to monitor binding reaction on different regions of chip.

scholarly journals Label-free detection of anti-estrogen receptor alpha and its binding with estrogen receptor peptide alpha by terahertz spectroscopy

RSC Advances ◽  
2017 ◽  
Vol 7 (39) ◽  
pp. 24338-24344 ◽  
Author(s):  
Mingliang Li ◽  
Tianying Chang ◽  
Dongshan Wei ◽  
Mingjie Tang ◽  
Shihan Yan ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Alpha ◽  
Terahertz Spectroscopy ◽  
Hydration Shell ◽  
Spectroscopic Techniques ◽  
Shell Formation ◽  
Label Free ◽  
Binding Reaction ◽  
Receptor Alpha ◽  
Label Free Detection

Terahertz (THz) spectroscopic techniques were employed to study the hydration shell formation around anti-estrogen receptor alpha (AER-α) and to detect the binding reaction between AER-α and estrogen receptor peptide alpha (ERP-α).

Label-Free Aptamer Fluorescence Determination of Trace Pb2+ Using AuPd Nanoalloy Probe as Catalyst

2013 ◽  
Vol 631-632 ◽  
pp. 18-21 ◽  
Author(s):  
Zhi Liang Jiang ◽  
Mei Ling Tang ◽  
Qing Ye Liu ◽  
Ai Hui Liang
Keyword(s):  
Detection Limit ◽  
Fluorescence Quenching ◽  
Fluorescence Intensity ◽  
Water Samples ◽  
Catalytic Effect ◽  
Rhodamine 6G ◽  
Label Free ◽  
Double Stranded Dna ◽  
Fluorescence Determination

In the condition of 1.24 mmol/L EDTANa2, 16.7 mmol/L NaCl and 0.17 mmol/L Tris, the substrate chain of double-stranded DNA (dsDNA) could be cracked by Pb2+ to release single-stranded DNA (ssDNA) that adsorb onto AuPd nanoparticle (AuPdNP) and form stable AuPdNP-ssDNA, but the dsDNA can not protect AuPdNP that were aggregated to big AuPdNP aggregations (AuPdNPA) under the action of NaCl. The AuPdNP-ssDNA and AuPdNPA could be separated by centrifugation. With the concentration of Pb2+ increased, the released ssDNA increased, the AuPdNP-ssDNA in centrifugation solution increased and the catalytic effect enhanced on the fluorescence quenching reaction of Rhodamine 6G (Rh6G) and NaH2PO2, which led the fluorescence intensity at 552nm to decrease. The decreased fluorescence intensity (ΔF552nm) was linear to the concentration of Pb2+ in the range of 0.33-8.00 nmol/L, a detection limit of 0.21 nmol/L. The proposed method was applied to detect Pb2+ in water samples, with satisfactory results.

scholarly journals Label-Free Sensitive Detection of Steroid Hormone Cortisol Based on Target-Induced Fluorescence Quenching of Quantum Dots

Langmuir ◽  
2020 ◽  
Vol 36 (27) ◽  
pp. 7781-7788
Author(s):  
Ye Liu ◽  
Bo Wu ◽  
Ekembu K. Tanyi ◽  
Sanjida Yeasmin ◽  
Li-Jing Cheng
Keyword(s):  
Quantum Dots ◽  
Fluorescence Quenching ◽  
Steroid Hormone ◽  
Sensitive Detection ◽  
Label Free

scholarly journals Fluorescence Enhancement of Fluorescent Unnatural Streptavidin by Binding of a Biotin Analogue with Spacer Tail and Its Application to Biotin Sensing

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Xianwei Zhu ◽  
Hiroaki Shinohara
Keyword(s):  
Amino Acid ◽  
Fluorescence Quenching ◽  
Fluorescence Intensity ◽  
Fluorescence Enhancement ◽  
Binding Protein ◽  
Unnatural Amino Acid ◽  
Competitive Binding ◽  
Binding Reaction ◽  
Biosensing System

We designed a novel molecular biosensing system for the detection of biotin, an important vitamin by the combination of fluorescent unnatural streptavidin with a commercialized biotin-(AC5)2-hydrazide. A fluorescent unnatural amino acid, BODIPY-FL-aminophenylalanine (BFLAF), was position-specifically incorporated into Trp120 of streptavidin by four-base codon method. Fluorescence of the Trp120BFLAF mutant streptavidin was enhanced by the addition of biotin-(AC5)2-hydrazide with the concentration dependent, whereas fluorescence enhancement was not observed at all by the addition of natural biotin. It was considered that the spacer tail of biotin-(AC5)2-hydrazide may disturb the fluorescence quenching of the Trp120BFLAF by Trp79 and Trp108 of the neighbor subunit. Therefore, biotin sensing was carried out by the competitive binding reaction of biotin-(AC5)2-hydrazide and natural biotin to the fluorescent mutant streptavidin. The fluorescence intensity decreased by increasing free biotin concentration. The result suggested that molecular biosensor for small ligand could be successfully designed by the pair of fluorescent mutant binding protein and ligand analogue.

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