An ultrasensitive fluorescent aptasensor for adenosine detection based on exonuclease III assisted signal amplification

2012 ◽  
Vol 34 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Peng Hu ◽  
Chengzhou Zhu ◽  
Lihua Jin ◽  
Shaojun Dong
Keyword(s):  
Signal Amplification ◽  
Exonuclease Iii ◽  
Fluorescent Aptasensor

A fluorescent aptasensor with product-triggered amplification by exonuclease III digestion for highly sensitive ATP detection

2017 ◽  
Vol 9 (33) ◽  
pp. 4837-4842 ◽  
Author(s):  
Shengnan He ◽  
Long Qu ◽  
Ying Tan ◽  
Feng Liu ◽  
Yu Wang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Exonuclease Iii ◽  
Digestion Product ◽  
Detection Assay ◽  
Highly Sensitive ◽  
Atp Detection ◽  
Fluorescent Aptasensor

An improved sensitivity of an ATP detection assay was achieved by using an allosteric probe and exonuclease III digestion product-triggered signal amplification.

scholarly journals Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

Nanomaterials ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 104
Author(s):  
Fuyuan Zhang ◽  
Linyang Liu ◽  
Shengnan Ni ◽  
Jiankang Deng ◽  
Guo-Jun Liu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Aflatoxin M1 ◽  
Exonuclease Iii ◽  
Sensor Performance ◽  
Milk Samples ◽  
Increased Sensitivity ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Optimized Conditions ◽  
Magnetic Nanobeads

In order to satisfy the need for sensitive detection of Aflatoxin M1 (AFM1), we constructed a simple and signal-on fluorescence aptasensor based on an autocatalytic Exonuclease III (Exo III)-assisted signal amplification strategy. In this sensor, the DNA hybridization on magnetic nanobeads could be triggered by the target AFM1, resulting in the release of a single-stranded DNA to induce an Exo III-assisted signal amplification, in which numerous G-quadruplex structures would be produced and then associated with the fluorescent dye to generate significantly amplified fluorescence signals resulting in the increased sensitivity. Under the optimized conditions, this aptasensor was able to detect AFM1 with a practical detection limit of 9.73 ng kg−1 in milk samples. Furthermore, the prepared sensor was successfully used for detection of AFM1 in the commercially available milk samples with the recovery percentages ranging from 80.13% to 108.67%. Also, the sensor performance was evaluated by the commercial immunoassay kit with satisfactory results.

Multi-signal amplification electrochemical DNA biosensor based on exonuclease III and tetraferrocene

2020 ◽  
Vol 8 (18) ◽  
pp. 4143-4150
Author(s):  
Zhaojiang Yin ◽  
Hanfeng Cui ◽  
Qingxia Shu ◽  
Chen Jin ◽  
Yan Lin ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Electrochemical Sensor ◽  
Signal Amplification ◽  
Dna Biosensor ◽  
Exonuclease Iii ◽  
Electrochemical Dna Biosensor ◽  
Exo Iii

A new and efficient signal marker, 3,5-bis(3,5-bisferrocenethoxybenzyloxy)benzoic acid, was firstly synthesized and used for labeling. Construct a homogeneous electrochemical sensor with Exo III assisting the target cycle.

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